Cargando…
A Quorum Sensing Regulated Small Volatile Molecule Reduces Acute Virulence and Promotes Chronic Infection Phenotypes
A significant number of environmental microorganisms can cause serious, even fatal, acute and chronic infections in humans. The severity and outcome of each type of infection depends on the expression of specific bacterial phenotypes controlled by complex regulatory networks that sense and respond t...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3150319/ https://www.ncbi.nlm.nih.gov/pubmed/21829370 http://dx.doi.org/10.1371/journal.ppat.1002192 |
_version_ | 1782209531246280704 |
---|---|
author | Kesarwani, Meenu Hazan, Ronen He, Jianxin Que, YokAi Apidianakis, Yiorgos Lesic, Biliana Xiao, Gaoping Dekimpe, Valérie Milot, Sylvain Deziel, Eric Lépine, François Rahme, Laurence G. |
author_facet | Kesarwani, Meenu Hazan, Ronen He, Jianxin Que, YokAi Apidianakis, Yiorgos Lesic, Biliana Xiao, Gaoping Dekimpe, Valérie Milot, Sylvain Deziel, Eric Lépine, François Rahme, Laurence G. |
author_sort | Kesarwani, Meenu |
collection | PubMed |
description | A significant number of environmental microorganisms can cause serious, even fatal, acute and chronic infections in humans. The severity and outcome of each type of infection depends on the expression of specific bacterial phenotypes controlled by complex regulatory networks that sense and respond to the host environment. Although bacterial signals that contribute to a successful acute infection have been identified in a number of pathogens, the signals that mediate the onset and establishment of chronic infections have yet to be discovered. We identified a volatile, low molecular weight molecule, 2-amino acetophenone (2-AA), produced by the opportunistic human pathogen Pseudomonas aeruginosa that reduces bacterial virulence in vivo in flies and in an acute mouse infection model. 2-AA modulates the activity of the virulence regulator MvfR (multiple virulence factor regulator) via a negative feedback loop and it promotes the emergence of P. aeruginosa phenotypes that likely promote chronic lung infections, including accumulation of lasR mutants, long-term survival at stationary phase, and persistence in a Drosophila infection model. We report for the first time the existence of a quorum sensing (QS) regulated volatile molecule that induces bistability phenotype by stochastically silencing acute virulence functions in P. aeruginosa. We propose that 2-AA mediates changes in a subpopulation of cells that facilitate the exploitation of dynamic host environments and promote gene expression changes that favor chronic infections. |
format | Online Article Text |
id | pubmed-3150319 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-31503192011-08-09 A Quorum Sensing Regulated Small Volatile Molecule Reduces Acute Virulence and Promotes Chronic Infection Phenotypes Kesarwani, Meenu Hazan, Ronen He, Jianxin Que, YokAi Apidianakis, Yiorgos Lesic, Biliana Xiao, Gaoping Dekimpe, Valérie Milot, Sylvain Deziel, Eric Lépine, François Rahme, Laurence G. PLoS Pathog Research Article A significant number of environmental microorganisms can cause serious, even fatal, acute and chronic infections in humans. The severity and outcome of each type of infection depends on the expression of specific bacterial phenotypes controlled by complex regulatory networks that sense and respond to the host environment. Although bacterial signals that contribute to a successful acute infection have been identified in a number of pathogens, the signals that mediate the onset and establishment of chronic infections have yet to be discovered. We identified a volatile, low molecular weight molecule, 2-amino acetophenone (2-AA), produced by the opportunistic human pathogen Pseudomonas aeruginosa that reduces bacterial virulence in vivo in flies and in an acute mouse infection model. 2-AA modulates the activity of the virulence regulator MvfR (multiple virulence factor regulator) via a negative feedback loop and it promotes the emergence of P. aeruginosa phenotypes that likely promote chronic lung infections, including accumulation of lasR mutants, long-term survival at stationary phase, and persistence in a Drosophila infection model. We report for the first time the existence of a quorum sensing (QS) regulated volatile molecule that induces bistability phenotype by stochastically silencing acute virulence functions in P. aeruginosa. We propose that 2-AA mediates changes in a subpopulation of cells that facilitate the exploitation of dynamic host environments and promote gene expression changes that favor chronic infections. Public Library of Science 2011-08-04 /pmc/articles/PMC3150319/ /pubmed/21829370 http://dx.doi.org/10.1371/journal.ppat.1002192 Text en Kesarwani et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Kesarwani, Meenu Hazan, Ronen He, Jianxin Que, YokAi Apidianakis, Yiorgos Lesic, Biliana Xiao, Gaoping Dekimpe, Valérie Milot, Sylvain Deziel, Eric Lépine, François Rahme, Laurence G. A Quorum Sensing Regulated Small Volatile Molecule Reduces Acute Virulence and Promotes Chronic Infection Phenotypes |
title | A Quorum Sensing Regulated Small Volatile Molecule Reduces Acute Virulence and Promotes Chronic Infection Phenotypes |
title_full | A Quorum Sensing Regulated Small Volatile Molecule Reduces Acute Virulence and Promotes Chronic Infection Phenotypes |
title_fullStr | A Quorum Sensing Regulated Small Volatile Molecule Reduces Acute Virulence and Promotes Chronic Infection Phenotypes |
title_full_unstemmed | A Quorum Sensing Regulated Small Volatile Molecule Reduces Acute Virulence and Promotes Chronic Infection Phenotypes |
title_short | A Quorum Sensing Regulated Small Volatile Molecule Reduces Acute Virulence and Promotes Chronic Infection Phenotypes |
title_sort | quorum sensing regulated small volatile molecule reduces acute virulence and promotes chronic infection phenotypes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3150319/ https://www.ncbi.nlm.nih.gov/pubmed/21829370 http://dx.doi.org/10.1371/journal.ppat.1002192 |
work_keys_str_mv | AT kesarwanimeenu aquorumsensingregulatedsmallvolatilemoleculereducesacutevirulenceandpromoteschronicinfectionphenotypes AT hazanronen aquorumsensingregulatedsmallvolatilemoleculereducesacutevirulenceandpromoteschronicinfectionphenotypes AT hejianxin aquorumsensingregulatedsmallvolatilemoleculereducesacutevirulenceandpromoteschronicinfectionphenotypes AT queyokai aquorumsensingregulatedsmallvolatilemoleculereducesacutevirulenceandpromoteschronicinfectionphenotypes AT apidianakisyiorgos aquorumsensingregulatedsmallvolatilemoleculereducesacutevirulenceandpromoteschronicinfectionphenotypes AT lesicbiliana aquorumsensingregulatedsmallvolatilemoleculereducesacutevirulenceandpromoteschronicinfectionphenotypes AT xiaogaoping aquorumsensingregulatedsmallvolatilemoleculereducesacutevirulenceandpromoteschronicinfectionphenotypes AT dekimpevalerie aquorumsensingregulatedsmallvolatilemoleculereducesacutevirulenceandpromoteschronicinfectionphenotypes AT milotsylvain aquorumsensingregulatedsmallvolatilemoleculereducesacutevirulenceandpromoteschronicinfectionphenotypes AT dezieleric aquorumsensingregulatedsmallvolatilemoleculereducesacutevirulenceandpromoteschronicinfectionphenotypes AT lepinefrancois aquorumsensingregulatedsmallvolatilemoleculereducesacutevirulenceandpromoteschronicinfectionphenotypes AT rahmelaurenceg aquorumsensingregulatedsmallvolatilemoleculereducesacutevirulenceandpromoteschronicinfectionphenotypes AT kesarwanimeenu quorumsensingregulatedsmallvolatilemoleculereducesacutevirulenceandpromoteschronicinfectionphenotypes AT hazanronen quorumsensingregulatedsmallvolatilemoleculereducesacutevirulenceandpromoteschronicinfectionphenotypes AT hejianxin quorumsensingregulatedsmallvolatilemoleculereducesacutevirulenceandpromoteschronicinfectionphenotypes AT queyokai quorumsensingregulatedsmallvolatilemoleculereducesacutevirulenceandpromoteschronicinfectionphenotypes AT apidianakisyiorgos quorumsensingregulatedsmallvolatilemoleculereducesacutevirulenceandpromoteschronicinfectionphenotypes AT lesicbiliana quorumsensingregulatedsmallvolatilemoleculereducesacutevirulenceandpromoteschronicinfectionphenotypes AT xiaogaoping quorumsensingregulatedsmallvolatilemoleculereducesacutevirulenceandpromoteschronicinfectionphenotypes AT dekimpevalerie quorumsensingregulatedsmallvolatilemoleculereducesacutevirulenceandpromoteschronicinfectionphenotypes AT milotsylvain quorumsensingregulatedsmallvolatilemoleculereducesacutevirulenceandpromoteschronicinfectionphenotypes AT dezieleric quorumsensingregulatedsmallvolatilemoleculereducesacutevirulenceandpromoteschronicinfectionphenotypes AT lepinefrancois quorumsensingregulatedsmallvolatilemoleculereducesacutevirulenceandpromoteschronicinfectionphenotypes AT rahmelaurenceg quorumsensingregulatedsmallvolatilemoleculereducesacutevirulenceandpromoteschronicinfectionphenotypes |