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Biodegradable Polymers Induce CD54 on THP-1 Cells in Skin Sensitization Test
Currently, nonanimal methods of skin sensitization testing for various chemicals, biodegradable polymers, and biomaterials are being developed in the hope of eliminating the use of animals. The human cell line activation test (h-CLAT) is a skin sensitization assessment that mimics the functions of d...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3150779/ https://www.ncbi.nlm.nih.gov/pubmed/21826143 http://dx.doi.org/10.1155/2011/424571 |
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author | Jung, Yeon Suk Kato, Reiko Tsuchiya, Toshie |
author_facet | Jung, Yeon Suk Kato, Reiko Tsuchiya, Toshie |
author_sort | Jung, Yeon Suk |
collection | PubMed |
description | Currently, nonanimal methods of skin sensitization testing for various chemicals, biodegradable polymers, and biomaterials are being developed in the hope of eliminating the use of animals. The human cell line activation test (h-CLAT) is a skin sensitization assessment that mimics the functions of dendritic cells (DCs). DCs are specialized antigen-presenting cells, and they interact with T cells and B cells to initiate immune responses. Phenotypic changes in DCs, such as the production of CD86 and CD54 and internalization of MHC class II molecules, have become focal points of the skin sensitization test. In this study, we used h-CLAT to assess the effects of biodegradable polymers. The results showed that several biodegradable polymers increased the expression of CD54, and the relative skin sensitizing abilities of biodegradable polymers were PLLG (75 : 25) < PLLC (40 : 60) < PLGA (50 : 50) < PCG (50 : 50). These results may contribute to the creation of new guidelines for the use of biodegradable polymers in scaffolds or allergenic hazards. |
format | Online Article Text |
id | pubmed-3150779 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-31507792011-08-08 Biodegradable Polymers Induce CD54 on THP-1 Cells in Skin Sensitization Test Jung, Yeon Suk Kato, Reiko Tsuchiya, Toshie Int J Biomater Research Article Currently, nonanimal methods of skin sensitization testing for various chemicals, biodegradable polymers, and biomaterials are being developed in the hope of eliminating the use of animals. The human cell line activation test (h-CLAT) is a skin sensitization assessment that mimics the functions of dendritic cells (DCs). DCs are specialized antigen-presenting cells, and they interact with T cells and B cells to initiate immune responses. Phenotypic changes in DCs, such as the production of CD86 and CD54 and internalization of MHC class II molecules, have become focal points of the skin sensitization test. In this study, we used h-CLAT to assess the effects of biodegradable polymers. The results showed that several biodegradable polymers increased the expression of CD54, and the relative skin sensitizing abilities of biodegradable polymers were PLLG (75 : 25) < PLLC (40 : 60) < PLGA (50 : 50) < PCG (50 : 50). These results may contribute to the creation of new guidelines for the use of biodegradable polymers in scaffolds or allergenic hazards. Hindawi Publishing Corporation 2011 2011-08-02 /pmc/articles/PMC3150779/ /pubmed/21826143 http://dx.doi.org/10.1155/2011/424571 Text en Copyright © 2011 Yeon Suk Jung et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Jung, Yeon Suk Kato, Reiko Tsuchiya, Toshie Biodegradable Polymers Induce CD54 on THP-1 Cells in Skin Sensitization Test |
title | Biodegradable Polymers Induce CD54 on THP-1 Cells in Skin Sensitization Test |
title_full | Biodegradable Polymers Induce CD54 on THP-1 Cells in Skin Sensitization Test |
title_fullStr | Biodegradable Polymers Induce CD54 on THP-1 Cells in Skin Sensitization Test |
title_full_unstemmed | Biodegradable Polymers Induce CD54 on THP-1 Cells in Skin Sensitization Test |
title_short | Biodegradable Polymers Induce CD54 on THP-1 Cells in Skin Sensitization Test |
title_sort | biodegradable polymers induce cd54 on thp-1 cells in skin sensitization test |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3150779/ https://www.ncbi.nlm.nih.gov/pubmed/21826143 http://dx.doi.org/10.1155/2011/424571 |
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