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Biodegradable Polymers Induce CD54 on THP-1 Cells in Skin Sensitization Test

Currently, nonanimal methods of skin sensitization testing for various chemicals, biodegradable polymers, and biomaterials are being developed in the hope of eliminating the use of animals. The human cell line activation test (h-CLAT) is a skin sensitization assessment that mimics the functions of d...

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Detalles Bibliográficos
Autores principales: Jung, Yeon Suk, Kato, Reiko, Tsuchiya, Toshie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3150779/
https://www.ncbi.nlm.nih.gov/pubmed/21826143
http://dx.doi.org/10.1155/2011/424571
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author Jung, Yeon Suk
Kato, Reiko
Tsuchiya, Toshie
author_facet Jung, Yeon Suk
Kato, Reiko
Tsuchiya, Toshie
author_sort Jung, Yeon Suk
collection PubMed
description Currently, nonanimal methods of skin sensitization testing for various chemicals, biodegradable polymers, and biomaterials are being developed in the hope of eliminating the use of animals. The human cell line activation test (h-CLAT) is a skin sensitization assessment that mimics the functions of dendritic cells (DCs). DCs are specialized antigen-presenting cells, and they interact with T cells and B cells to initiate immune responses. Phenotypic changes in DCs, such as the production of CD86 and CD54 and internalization of MHC class II molecules, have become focal points of the skin sensitization test. In this study, we used h-CLAT to assess the effects of biodegradable polymers. The results showed that several biodegradable polymers increased the expression of CD54, and the relative skin sensitizing abilities of biodegradable polymers were PLLG (75 : 25) < PLLC (40 : 60) < PLGA (50 : 50) < PCG (50 : 50). These results may contribute to the creation of new guidelines for the use of biodegradable polymers in scaffolds or allergenic hazards.
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spelling pubmed-31507792011-08-08 Biodegradable Polymers Induce CD54 on THP-1 Cells in Skin Sensitization Test Jung, Yeon Suk Kato, Reiko Tsuchiya, Toshie Int J Biomater Research Article Currently, nonanimal methods of skin sensitization testing for various chemicals, biodegradable polymers, and biomaterials are being developed in the hope of eliminating the use of animals. The human cell line activation test (h-CLAT) is a skin sensitization assessment that mimics the functions of dendritic cells (DCs). DCs are specialized antigen-presenting cells, and they interact with T cells and B cells to initiate immune responses. Phenotypic changes in DCs, such as the production of CD86 and CD54 and internalization of MHC class II molecules, have become focal points of the skin sensitization test. In this study, we used h-CLAT to assess the effects of biodegradable polymers. The results showed that several biodegradable polymers increased the expression of CD54, and the relative skin sensitizing abilities of biodegradable polymers were PLLG (75 : 25) < PLLC (40 : 60) < PLGA (50 : 50) < PCG (50 : 50). These results may contribute to the creation of new guidelines for the use of biodegradable polymers in scaffolds or allergenic hazards. Hindawi Publishing Corporation 2011 2011-08-02 /pmc/articles/PMC3150779/ /pubmed/21826143 http://dx.doi.org/10.1155/2011/424571 Text en Copyright © 2011 Yeon Suk Jung et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Jung, Yeon Suk
Kato, Reiko
Tsuchiya, Toshie
Biodegradable Polymers Induce CD54 on THP-1 Cells in Skin Sensitization Test
title Biodegradable Polymers Induce CD54 on THP-1 Cells in Skin Sensitization Test
title_full Biodegradable Polymers Induce CD54 on THP-1 Cells in Skin Sensitization Test
title_fullStr Biodegradable Polymers Induce CD54 on THP-1 Cells in Skin Sensitization Test
title_full_unstemmed Biodegradable Polymers Induce CD54 on THP-1 Cells in Skin Sensitization Test
title_short Biodegradable Polymers Induce CD54 on THP-1 Cells in Skin Sensitization Test
title_sort biodegradable polymers induce cd54 on thp-1 cells in skin sensitization test
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3150779/
https://www.ncbi.nlm.nih.gov/pubmed/21826143
http://dx.doi.org/10.1155/2011/424571
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