Simple, Time-Saving Dye Staining of Proteins for Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis Using Coomassie Blue

A fixation-free and fast protein-staining method for sodium dodecyl sulfate–polyacrylamide gel electrophoresis using Coomassie blue is described. The protocol comprises staining and quick washing steps, which can be completed in 0.5 h. It has a sensitivity of 10 ng, comparable with that of conventio...

Descripción completa

Detalles Bibliográficos
Autores principales: Dong, Wei-Hua, Wang, Tian-Yun, Wang, Fang, Zhang, Jun-He
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3151240/
https://www.ncbi.nlm.nih.gov/pubmed/21850222
http://dx.doi.org/10.1371/journal.pone.0022394
_version_ 1782209593585172480
author Dong, Wei-Hua
Wang, Tian-Yun
Wang, Fang
Zhang, Jun-He
author_facet Dong, Wei-Hua
Wang, Tian-Yun
Wang, Fang
Zhang, Jun-He
author_sort Dong, Wei-Hua
collection PubMed
description A fixation-free and fast protein-staining method for sodium dodecyl sulfate–polyacrylamide gel electrophoresis using Coomassie blue is described. The protocol comprises staining and quick washing steps, which can be completed in 0.5 h. It has a sensitivity of 10 ng, comparable with that of conventional Coomassie Brilliant Blue G staining with phosphoric acid in the staining solution. In addition, the dye stain does not contain any amount of acid and methanol, such as phosphoric acid. Considering the speed, simplicity, and low cost, the dye stain may be of more practical value than other dye-based protein stains in routine proteomic research.
format Online
Article
Text
id pubmed-3151240
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-31512402011-08-17 Simple, Time-Saving Dye Staining of Proteins for Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis Using Coomassie Blue Dong, Wei-Hua Wang, Tian-Yun Wang, Fang Zhang, Jun-He PLoS One Research Article A fixation-free and fast protein-staining method for sodium dodecyl sulfate–polyacrylamide gel electrophoresis using Coomassie blue is described. The protocol comprises staining and quick washing steps, which can be completed in 0.5 h. It has a sensitivity of 10 ng, comparable with that of conventional Coomassie Brilliant Blue G staining with phosphoric acid in the staining solution. In addition, the dye stain does not contain any amount of acid and methanol, such as phosphoric acid. Considering the speed, simplicity, and low cost, the dye stain may be of more practical value than other dye-based protein stains in routine proteomic research. Public Library of Science 2011-08-05 /pmc/articles/PMC3151240/ /pubmed/21850222 http://dx.doi.org/10.1371/journal.pone.0022394 Text en This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication. https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
spellingShingle Research Article
Dong, Wei-Hua
Wang, Tian-Yun
Wang, Fang
Zhang, Jun-He
Simple, Time-Saving Dye Staining of Proteins for Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis Using Coomassie Blue
title Simple, Time-Saving Dye Staining of Proteins for Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis Using Coomassie Blue
title_full Simple, Time-Saving Dye Staining of Proteins for Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis Using Coomassie Blue
title_fullStr Simple, Time-Saving Dye Staining of Proteins for Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis Using Coomassie Blue
title_full_unstemmed Simple, Time-Saving Dye Staining of Proteins for Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis Using Coomassie Blue
title_short Simple, Time-Saving Dye Staining of Proteins for Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis Using Coomassie Blue
title_sort simple, time-saving dye staining of proteins for sodium dodecyl sulfate–polyacrylamide gel electrophoresis using coomassie blue
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3151240/
https://www.ncbi.nlm.nih.gov/pubmed/21850222
http://dx.doi.org/10.1371/journal.pone.0022394
work_keys_str_mv AT dongweihua simpletimesavingdyestainingofproteinsforsodiumdodecylsulfatepolyacrylamidegelelectrophoresisusingcoomassieblue
AT wangtianyun simpletimesavingdyestainingofproteinsforsodiumdodecylsulfatepolyacrylamidegelelectrophoresisusingcoomassieblue
AT wangfang simpletimesavingdyestainingofproteinsforsodiumdodecylsulfatepolyacrylamidegelelectrophoresisusingcoomassieblue
AT zhangjunhe simpletimesavingdyestainingofproteinsforsodiumdodecylsulfatepolyacrylamidegelelectrophoresisusingcoomassieblue

Ejemplares similares