Simple, Time-Saving Dye Staining of Proteins for Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis Using Coomassie Blue

A fixation-free and fast protein-staining method for sodium dodecyl sulfate–polyacrylamide gel electrophoresis using Coomassie blue is described. The protocol comprises staining and quick washing steps, which can be completed in 0.5 h. It has a sensitivity of 10 ng, comparable with that of conventio...

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Detalles Bibliográficos
Autores principales: Dong, Wei-Hua, Wang, Tian-Yun, Wang, Fang, Zhang, Jun-He
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3151240/
https://www.ncbi.nlm.nih.gov/pubmed/21850222
http://dx.doi.org/10.1371/journal.pone.0022394

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3151240/
https://www.ncbi.nlm.nih.gov/pubmed/21850222
http://dx.doi.org/10.1371/journal.pone.0022394

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