Cargando…
Imaging Single Photons and Intrinsic Optical Signals for Studies of Vesicular and Non-Vesicular ATP Release from Axons
The temporal and spatial dynamics of neurotransmitter release are fundamental to understanding activity-dependent signaling between axons and other cells, including neurons, glia, and vascular cells. A microscopic imaging technique is described that enables studying release of the neurotransmitter A...
Autor principal: | |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Research Foundation
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3151621/ https://www.ncbi.nlm.nih.gov/pubmed/21852965 http://dx.doi.org/10.3389/fnana.2011.00032 |
_version_ | 1782209637925257216 |
---|---|
author | Fields, R. Douglas |
author_facet | Fields, R. Douglas |
author_sort | Fields, R. Douglas |
collection | PubMed |
description | The temporal and spatial dynamics of neurotransmitter release are fundamental to understanding activity-dependent signaling between axons and other cells, including neurons, glia, and vascular cells. A microscopic imaging technique is described that enables studying release of the neurotransmitter ATP from axons in response to action potentials. The method combines imaging single-photons, intrinsic optical signal imaging, and high magnification time-lapse microcopy to enable investigations of action potential-induced ATP release together with cell morphology and activity-dependent axon swelling. ATP released from axons catalyzes a chemiluminescent reaction between luciferin and luciferase that generates single photons that can be imaged individually. In addition to vesicular release, ATP release through membrane channels activated by axon swelling was monitored simultaneously with intrinsic optical signals. Repeated emissions of photons were observed from localized 15 μm regions of axons, with a frequency distribution that differed from a normal distribution and from the frequency of emissions outside these localized regions. |
format | Online Article Text |
id | pubmed-3151621 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Frontiers Research Foundation |
record_format | MEDLINE/PubMed |
spelling | pubmed-31516212011-08-18 Imaging Single Photons and Intrinsic Optical Signals for Studies of Vesicular and Non-Vesicular ATP Release from Axons Fields, R. Douglas Front Neuroanat Neuroscience The temporal and spatial dynamics of neurotransmitter release are fundamental to understanding activity-dependent signaling between axons and other cells, including neurons, glia, and vascular cells. A microscopic imaging technique is described that enables studying release of the neurotransmitter ATP from axons in response to action potentials. The method combines imaging single-photons, intrinsic optical signal imaging, and high magnification time-lapse microcopy to enable investigations of action potential-induced ATP release together with cell morphology and activity-dependent axon swelling. ATP released from axons catalyzes a chemiluminescent reaction between luciferin and luciferase that generates single photons that can be imaged individually. In addition to vesicular release, ATP release through membrane channels activated by axon swelling was monitored simultaneously with intrinsic optical signals. Repeated emissions of photons were observed from localized 15 μm regions of axons, with a frequency distribution that differed from a normal distribution and from the frequency of emissions outside these localized regions. Frontiers Research Foundation 2011-06-06 /pmc/articles/PMC3151621/ /pubmed/21852965 http://dx.doi.org/10.3389/fnana.2011.00032 Text en Copyright © 2011 Fields. http://www.frontiersin.org/licenseagreement This is an open-access article subject to a non-exclusive license between the authors and Frontiers Media SA, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and other Frontiers conditions are complied with. |
spellingShingle | Neuroscience Fields, R. Douglas Imaging Single Photons and Intrinsic Optical Signals for Studies of Vesicular and Non-Vesicular ATP Release from Axons |
title | Imaging Single Photons and Intrinsic Optical Signals for Studies of Vesicular and Non-Vesicular ATP Release from Axons |
title_full | Imaging Single Photons and Intrinsic Optical Signals for Studies of Vesicular and Non-Vesicular ATP Release from Axons |
title_fullStr | Imaging Single Photons and Intrinsic Optical Signals for Studies of Vesicular and Non-Vesicular ATP Release from Axons |
title_full_unstemmed | Imaging Single Photons and Intrinsic Optical Signals for Studies of Vesicular and Non-Vesicular ATP Release from Axons |
title_short | Imaging Single Photons and Intrinsic Optical Signals for Studies of Vesicular and Non-Vesicular ATP Release from Axons |
title_sort | imaging single photons and intrinsic optical signals for studies of vesicular and non-vesicular atp release from axons |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3151621/ https://www.ncbi.nlm.nih.gov/pubmed/21852965 http://dx.doi.org/10.3389/fnana.2011.00032 |
work_keys_str_mv | AT fieldsrdouglas imagingsinglephotonsandintrinsicopticalsignalsforstudiesofvesicularandnonvesicularatpreleasefromaxons |