Cargando…

A rapid, inexpensive yeast-based dual-fluorescence assay of programmed—1 ribosomal frameshifting for high-throughput screening

Programmed −1 ribosomal frameshifting (−1 PRF) is a mechanism that directs elongating ribosomes to shift-reading frame by 1 base in the 5′ direction that is utilized by many RNA viruses. Importantly, rates of −1 PRF are fine-tuned by viruses, including Retroviruses, Coronaviruses, Flavivriuses and i...

Descripción completa

Detalles Bibliográficos
Autores principales:	Rakauskaitė, Rasa, Liao, Pei-Yu, Rhodin, Michael H. J., Lee, Kelvin, Dinman, Jonathan D.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2011
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3152369/ https://www.ncbi.nlm.nih.gov/pubmed/21602263 http://dx.doi.org/10.1093/nar/gkr382

Ejemplares similares

Identification of functional, endogenous programmed −1 ribosomal frameshift signals in the genome of Saccharomyces cerevisiae
por: Jacobs, Jonathan L., et al.
Publicado: (2007)

Fluorescent T7 display phages obtained by translational frameshift
por: Slootweg, Erik J., et al.
Publicado: (2006)

Characterization of L1 retrotransposition with high-throughput dual-luciferase assays
por: Xie, Yi, et al.
Publicado: (2011)

The many paths to frameshifting: kinetic modelling and analysis of the effects of different elongation steps on programmed –1 ribosomal frameshifting
por: Liao, Pei-Yu, et al.
Publicado: (2011)

rRNA mutants in the yeast peptidyltransferase center reveal allosteric information networks and mechanisms of drug resistance
por: Rakauskaitė, Rasa, et al.
Publicado: (2008)

A dual-fluorescence reporter system for high-throughput clone characterization and selection by cell sorting
por: Choe, Juno, et al.
Publicado: (2005)

Fluorescence monitoring of riboswitch transcription regulation using a dual molecular beacon assay
por: Chinnappan, Raja, et al.
Publicado: (2013)

An ultrasensitive site-specific DNA recombination assay based on dual-color fluorescence cross-correlation spectroscopy
por: Jahnz, Michael, et al.
Publicado: (2005)

Fluorescence strategies for high-throughput quantification of protein interactions
por: Hieb, Aaron R., et al.
Publicado: (2012)

Programmed –1 Ribosomal Frameshifting in SARS Coronavirus
por: Dinman, Jonathan D.
Publicado: (2009)

High-throughput assays for DNA gyrase and other topoisomerases
por: Maxwell, Anthony, et al.
Publicado: (2006)

Systematic analysis of bicistronic reporter assay data
por: Jacobs, Jonathan L., et al.
Publicado: (2004)

High-throughput assay and engineering of self-cleaving ribozymes by sequencing
por: Kobori, Shungo, et al.
Publicado: (2015)

RNAsnap™: a rapid, quantitative and inexpensive, method for isolating total RNA from bacteria
por: Stead, Mark B., et al.
Publicado: (2012)

Validation of a fluorescence-based screening concept to identify ribosome assembly defects in Escherichia coli
por: Nikolay, Rainer, et al.
Publicado: (2014)

A high-throughput assay for the comprehensive profiling of DNA ligase fidelity
por: Lohman, Gregory J. S., et al.
Publicado: (2016)

Novel cell surface luciferase reporter for high-throughput yeast one-hybrid screens
por: Bonaldi, Katia, et al.
Publicado: (2017)

Sensitive and inexpensive digital DNA analysis by microfluidic enrichment of rolling circle amplified single-molecules
por: Kühnemund, Malte, et al.
Publicado: (2017)

A new kinetic model reveals the synergistic effect of E-, P- and A-sites on +1 ribosomal frameshifting
por: Liao, Pei-Yu, et al.
Publicado: (2008)

Identifying ribosome heterogeneity using ribosome profiling
por: Alkan, Ferhat, et al.
Publicado: (2022)

An Extensive Network of Information Flow through the B1b/c Intersubunit Bridge of the Yeast Ribosome
por: Rhodin, Michael H. J., et al.
Publicado: (2011)

3′LIFE: a functional assay to detect miRNA targets in high-throughput
por: Wolter, Justin M., et al.
Publicado: (2014)

Dual FRET assay for detecting receptor protein interaction with DNA
por: Krusiński, Tomasz, et al.
Publicado: (2010)

Fluorescence protection assay: a novel homogeneous assay platform toward development of aptamer sensors for protein detection
por: Wang, Hong-Qi, et al.
Publicado: (2011)

Yeast telomere maintenance is globally controlled by programmed ribosomal frameshifting and the nonsense-mediated mRNA decay pathway
por: Advani, Vivek M., et al.
Publicado: (2013)

A flexible loop in yeast ribosomal protein L11 coordinates P-site tRNA binding
por: Rhodin, Michael H. J., et al.
Publicado: (2010)

pBT, a novel vector for tetracycline-regulated yeast three-hybrid assay
por: Moriyoshi, Koki
Publicado: (2009)

A fluorescence-based helicase assay: application to the screening of G-quadruplex ligands
por: Mendoza, Oscar, et al.
Publicado: (2015)

Engineering a ribozyme cleavage-induced split fluorescent aptamer complementation assay
por: Ausländer, Simon, et al.
Publicado: (2016)

A user-friendly, high-throughput tool for the precise fluorescent quantification of deoxyribonucleoside triphosphates from biological samples
por: Szabó, Judit Eszter, et al.
Publicado: (2020)

Endogenous ribosomal frameshift signals operate as mRNA destabilizing elements through at least two molecular pathways in yeast
por: Belew, Ashton T., et al.
Publicado: (2011)

A novel fluorescence-based assay for the rapid detection and quantification of cellular deoxyribonucleoside triphosphates
por: Wilson, Peter M., et al.
Publicado: (2011)

Diff-seq: A high throughput sequencing-based mismatch detection assay for DNA variant enrichment and discovery
por: Aggeli, Dimitra, et al.
Publicado: (2018)

Two-photon fluorescence cross-correlation spectroscopy as a potential tool for high-throughput screening of DNA repair activity
por: Collini, Maddalena, et al.
Publicado: (2005)

Magnetically assisted DNA assays: high selectivity using conjugated polymers for amplified fluorescent transduction
por: Xu, Hui, et al.
Publicado: (2005)

A real-time fluorescence polarization activity assay to screen for inhibitors of bacterial ribonuclease P
por: Liu, Xin, et al.
Publicado: (2014)

Programmed −1 Ribosomal Frameshifting in coronaviruses: A therapeutic target
por: Kelly, Jamie A., et al.
Publicado: (2021)

Ribonuclease selection for ribosome profiling
por: Gerashchenko, Maxim V., et al.
Publicado: (2017)

A novel high-throughput activity assay for the Trypanosoma brucei editosome enzyme REL1 and other RNA ligases
por: Zimmermann, Stephan, et al.
Publicado: (2016)

Development and application of a recombination-based library versus library high- throughput yeast two-hybrid (RLL-Y2H) screening system
por: Yang, Fang, et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_26ulqu9sisd2o7f5nqbc0mg0mb