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Effect of size and processing method on the cytotoxicity of realgar nanoparticles in cancer cell lines

In this study, the effects of the size and Chinese traditional processing (including elutriation, water cleaning, acid cleaning, alkali cleaning) on realgar nanoparticles (RN)-induced antitumor activity in human osteosarcoma cell lines (MG-63) and hepatoma carcinoma cell lines (HepG-2) were investig...

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Autores principales: Zhao, Weizhong, Lu, Xun, Yuan, Yuan, Liu, Changsheng, Yang, Baican, Hong, Hua, Wang, Guoying, Zeng, Fanyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3152475/
https://www.ncbi.nlm.nih.gov/pubmed/21845047
http://dx.doi.org/10.2147/IJN.S21373
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author Zhao, Weizhong
Lu, Xun
Yuan, Yuan
Liu, Changsheng
Yang, Baican
Hong, Hua
Wang, Guoying
Zeng, Fanyan
author_facet Zhao, Weizhong
Lu, Xun
Yuan, Yuan
Liu, Changsheng
Yang, Baican
Hong, Hua
Wang, Guoying
Zeng, Fanyan
author_sort Zhao, Weizhong
collection PubMed
description In this study, the effects of the size and Chinese traditional processing (including elutriation, water cleaning, acid cleaning, alkali cleaning) on realgar nanoparticles (RN)-induced antitumor activity in human osteosarcoma cell lines (MG-63) and hepatoma carcinoma cell lines (HepG-2) were investigated. The human normal liver cell line (L-02) was used as control. RN was prepared by high-energy ball milling technology. The results showed that with the assistance of sodium dodecyl sulfate, the size of realgar could be reduced to 127 nm after 12 hours’ ball milling. The surface charge was decreased from 0.83 eV to −17.85 eV and the content of As(2)O(3) clearly increased. Except for elutriation, the processing methods did not clearly change the size of the RN, but the content of As(2)O(3) was reduced dramatically. In vitro MTT tests indicated that in the two cancer cell lines, RN cytotoxicity was more intense than that of the coarse realgar nanoparticles, and cytotoxicity was typically time- and concentration-dependent. Also, RN cytotoxicities in the HepG-2 and L-02 cells all increased with increasing milling time. Due to the reduction of the As(2)O(3) content, water cleaning, acid cleaning, and alkali cleaning decreased RN cytotoxicity in HepG-2, but RN after elutriation, with the lowest As(2)O(3) (3.5 mg/g) and the smallest size (109.3 nm), showed comparable cytotoxicity in HepG-2 to RN without treatment. Meanwhile, RN-induced cytotoxicity in L-02 cells was clearly reduced. Therefore, it can be concluded that RN may provide a strong antiproliferation effect in the MG-63 and HepG-2 cells. Elutriation processing is a suitable approach to limit the dangerous side-effects of As(2)O(3), while maintaining the effectiveness of RN.
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spelling pubmed-31524752011-08-15 Effect of size and processing method on the cytotoxicity of realgar nanoparticles in cancer cell lines Zhao, Weizhong Lu, Xun Yuan, Yuan Liu, Changsheng Yang, Baican Hong, Hua Wang, Guoying Zeng, Fanyan Int J Nanomedicine Original Research In this study, the effects of the size and Chinese traditional processing (including elutriation, water cleaning, acid cleaning, alkali cleaning) on realgar nanoparticles (RN)-induced antitumor activity in human osteosarcoma cell lines (MG-63) and hepatoma carcinoma cell lines (HepG-2) were investigated. The human normal liver cell line (L-02) was used as control. RN was prepared by high-energy ball milling technology. The results showed that with the assistance of sodium dodecyl sulfate, the size of realgar could be reduced to 127 nm after 12 hours’ ball milling. The surface charge was decreased from 0.83 eV to −17.85 eV and the content of As(2)O(3) clearly increased. Except for elutriation, the processing methods did not clearly change the size of the RN, but the content of As(2)O(3) was reduced dramatically. In vitro MTT tests indicated that in the two cancer cell lines, RN cytotoxicity was more intense than that of the coarse realgar nanoparticles, and cytotoxicity was typically time- and concentration-dependent. Also, RN cytotoxicities in the HepG-2 and L-02 cells all increased with increasing milling time. Due to the reduction of the As(2)O(3) content, water cleaning, acid cleaning, and alkali cleaning decreased RN cytotoxicity in HepG-2, but RN after elutriation, with the lowest As(2)O(3) (3.5 mg/g) and the smallest size (109.3 nm), showed comparable cytotoxicity in HepG-2 to RN without treatment. Meanwhile, RN-induced cytotoxicity in L-02 cells was clearly reduced. Therefore, it can be concluded that RN may provide a strong antiproliferation effect in the MG-63 and HepG-2 cells. Elutriation processing is a suitable approach to limit the dangerous side-effects of As(2)O(3), while maintaining the effectiveness of RN. Dove Medical Press 2011 2011-08-02 /pmc/articles/PMC3152475/ /pubmed/21845047 http://dx.doi.org/10.2147/IJN.S21373 Text en © 2011 Zhao et al, publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited.
spellingShingle Original Research
Zhao, Weizhong
Lu, Xun
Yuan, Yuan
Liu, Changsheng
Yang, Baican
Hong, Hua
Wang, Guoying
Zeng, Fanyan
Effect of size and processing method on the cytotoxicity of realgar nanoparticles in cancer cell lines
title Effect of size and processing method on the cytotoxicity of realgar nanoparticles in cancer cell lines
title_full Effect of size and processing method on the cytotoxicity of realgar nanoparticles in cancer cell lines
title_fullStr Effect of size and processing method on the cytotoxicity of realgar nanoparticles in cancer cell lines
title_full_unstemmed Effect of size and processing method on the cytotoxicity of realgar nanoparticles in cancer cell lines
title_short Effect of size and processing method on the cytotoxicity of realgar nanoparticles in cancer cell lines
title_sort effect of size and processing method on the cytotoxicity of realgar nanoparticles in cancer cell lines
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3152475/
https://www.ncbi.nlm.nih.gov/pubmed/21845047
http://dx.doi.org/10.2147/IJN.S21373
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