Cargando…
Effect of size and processing method on the cytotoxicity of realgar nanoparticles in cancer cell lines
In this study, the effects of the size and Chinese traditional processing (including elutriation, water cleaning, acid cleaning, alkali cleaning) on realgar nanoparticles (RN)-induced antitumor activity in human osteosarcoma cell lines (MG-63) and hepatoma carcinoma cell lines (HepG-2) were investig...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3152475/ https://www.ncbi.nlm.nih.gov/pubmed/21845047 http://dx.doi.org/10.2147/IJN.S21373 |
_version_ | 1782209768777056256 |
---|---|
author | Zhao, Weizhong Lu, Xun Yuan, Yuan Liu, Changsheng Yang, Baican Hong, Hua Wang, Guoying Zeng, Fanyan |
author_facet | Zhao, Weizhong Lu, Xun Yuan, Yuan Liu, Changsheng Yang, Baican Hong, Hua Wang, Guoying Zeng, Fanyan |
author_sort | Zhao, Weizhong |
collection | PubMed |
description | In this study, the effects of the size and Chinese traditional processing (including elutriation, water cleaning, acid cleaning, alkali cleaning) on realgar nanoparticles (RN)-induced antitumor activity in human osteosarcoma cell lines (MG-63) and hepatoma carcinoma cell lines (HepG-2) were investigated. The human normal liver cell line (L-02) was used as control. RN was prepared by high-energy ball milling technology. The results showed that with the assistance of sodium dodecyl sulfate, the size of realgar could be reduced to 127 nm after 12 hours’ ball milling. The surface charge was decreased from 0.83 eV to −17.85 eV and the content of As(2)O(3) clearly increased. Except for elutriation, the processing methods did not clearly change the size of the RN, but the content of As(2)O(3) was reduced dramatically. In vitro MTT tests indicated that in the two cancer cell lines, RN cytotoxicity was more intense than that of the coarse realgar nanoparticles, and cytotoxicity was typically time- and concentration-dependent. Also, RN cytotoxicities in the HepG-2 and L-02 cells all increased with increasing milling time. Due to the reduction of the As(2)O(3) content, water cleaning, acid cleaning, and alkali cleaning decreased RN cytotoxicity in HepG-2, but RN after elutriation, with the lowest As(2)O(3) (3.5 mg/g) and the smallest size (109.3 nm), showed comparable cytotoxicity in HepG-2 to RN without treatment. Meanwhile, RN-induced cytotoxicity in L-02 cells was clearly reduced. Therefore, it can be concluded that RN may provide a strong antiproliferation effect in the MG-63 and HepG-2 cells. Elutriation processing is a suitable approach to limit the dangerous side-effects of As(2)O(3), while maintaining the effectiveness of RN. |
format | Online Article Text |
id | pubmed-3152475 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-31524752011-08-15 Effect of size and processing method on the cytotoxicity of realgar nanoparticles in cancer cell lines Zhao, Weizhong Lu, Xun Yuan, Yuan Liu, Changsheng Yang, Baican Hong, Hua Wang, Guoying Zeng, Fanyan Int J Nanomedicine Original Research In this study, the effects of the size and Chinese traditional processing (including elutriation, water cleaning, acid cleaning, alkali cleaning) on realgar nanoparticles (RN)-induced antitumor activity in human osteosarcoma cell lines (MG-63) and hepatoma carcinoma cell lines (HepG-2) were investigated. The human normal liver cell line (L-02) was used as control. RN was prepared by high-energy ball milling technology. The results showed that with the assistance of sodium dodecyl sulfate, the size of realgar could be reduced to 127 nm after 12 hours’ ball milling. The surface charge was decreased from 0.83 eV to −17.85 eV and the content of As(2)O(3) clearly increased. Except for elutriation, the processing methods did not clearly change the size of the RN, but the content of As(2)O(3) was reduced dramatically. In vitro MTT tests indicated that in the two cancer cell lines, RN cytotoxicity was more intense than that of the coarse realgar nanoparticles, and cytotoxicity was typically time- and concentration-dependent. Also, RN cytotoxicities in the HepG-2 and L-02 cells all increased with increasing milling time. Due to the reduction of the As(2)O(3) content, water cleaning, acid cleaning, and alkali cleaning decreased RN cytotoxicity in HepG-2, but RN after elutriation, with the lowest As(2)O(3) (3.5 mg/g) and the smallest size (109.3 nm), showed comparable cytotoxicity in HepG-2 to RN without treatment. Meanwhile, RN-induced cytotoxicity in L-02 cells was clearly reduced. Therefore, it can be concluded that RN may provide a strong antiproliferation effect in the MG-63 and HepG-2 cells. Elutriation processing is a suitable approach to limit the dangerous side-effects of As(2)O(3), while maintaining the effectiveness of RN. Dove Medical Press 2011 2011-08-02 /pmc/articles/PMC3152475/ /pubmed/21845047 http://dx.doi.org/10.2147/IJN.S21373 Text en © 2011 Zhao et al, publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited. |
spellingShingle | Original Research Zhao, Weizhong Lu, Xun Yuan, Yuan Liu, Changsheng Yang, Baican Hong, Hua Wang, Guoying Zeng, Fanyan Effect of size and processing method on the cytotoxicity of realgar nanoparticles in cancer cell lines |
title | Effect of size and processing method on the cytotoxicity of realgar nanoparticles in cancer cell lines |
title_full | Effect of size and processing method on the cytotoxicity of realgar nanoparticles in cancer cell lines |
title_fullStr | Effect of size and processing method on the cytotoxicity of realgar nanoparticles in cancer cell lines |
title_full_unstemmed | Effect of size and processing method on the cytotoxicity of realgar nanoparticles in cancer cell lines |
title_short | Effect of size and processing method on the cytotoxicity of realgar nanoparticles in cancer cell lines |
title_sort | effect of size and processing method on the cytotoxicity of realgar nanoparticles in cancer cell lines |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3152475/ https://www.ncbi.nlm.nih.gov/pubmed/21845047 http://dx.doi.org/10.2147/IJN.S21373 |
work_keys_str_mv | AT zhaoweizhong effectofsizeandprocessingmethodonthecytotoxicityofrealgarnanoparticlesincancercelllines AT luxun effectofsizeandprocessingmethodonthecytotoxicityofrealgarnanoparticlesincancercelllines AT yuanyuan effectofsizeandprocessingmethodonthecytotoxicityofrealgarnanoparticlesincancercelllines AT liuchangsheng effectofsizeandprocessingmethodonthecytotoxicityofrealgarnanoparticlesincancercelllines AT yangbaican effectofsizeandprocessingmethodonthecytotoxicityofrealgarnanoparticlesincancercelllines AT honghua effectofsizeandprocessingmethodonthecytotoxicityofrealgarnanoparticlesincancercelllines AT wangguoying effectofsizeandprocessingmethodonthecytotoxicityofrealgarnanoparticlesincancercelllines AT zengfanyan effectofsizeandprocessingmethodonthecytotoxicityofrealgarnanoparticlesincancercelllines |