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Transcriptome Sequencing Identifies PCAT-1, a Novel lincRNA Implicated in Prostate Cancer Progression
High-throughput sequencing of polyA+ RNA (RNA-Seq) in human cancer shows remarkable potential to identify both novel markers of disease and uncharacterized aspects of tumor biology, particularly non-coding RNA (ncRNA) species. We employed RNA-Seq on a cohort of 102 prostate tissues and cells lines a...
Autores principales: | , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3152676/ https://www.ncbi.nlm.nih.gov/pubmed/21804560 http://dx.doi.org/10.1038/nbt.1914 |
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author | Prensner, John R. Iyer, Matthew K. Balbin, O. Alejandro Dhanasekaran, Saravana M. Cao, Qi Brenner, J. Chad Laxman, Bharathi Asangani, Irfan Grasso, Catherine Kominsky, Hal D. Cao, Xuhong Jing, Xiaojun Wang, Xiaoju Siddiqui, Javed Wei, John T. Robinson, Daniel Iyer, Hari K. Palanisamy, Nallasivam Maher, Christopher A. Chinnaiyan, Arul M. |
author_facet | Prensner, John R. Iyer, Matthew K. Balbin, O. Alejandro Dhanasekaran, Saravana M. Cao, Qi Brenner, J. Chad Laxman, Bharathi Asangani, Irfan Grasso, Catherine Kominsky, Hal D. Cao, Xuhong Jing, Xiaojun Wang, Xiaoju Siddiqui, Javed Wei, John T. Robinson, Daniel Iyer, Hari K. Palanisamy, Nallasivam Maher, Christopher A. Chinnaiyan, Arul M. |
author_sort | Prensner, John R. |
collection | PubMed |
description | High-throughput sequencing of polyA+ RNA (RNA-Seq) in human cancer shows remarkable potential to identify both novel markers of disease and uncharacterized aspects of tumor biology, particularly non-coding RNA (ncRNA) species. We employed RNA-Seq on a cohort of 102 prostate tissues and cells lines and performed ab initio transcriptome assembly to discover unannotated ncRNAs. We nominated 121 such Prostate Cancer Associated Transcripts (PCATs) with cancer-specific expression patterns. Among these, we characterized PCAT-1 as a novel prostate-specific regulator of cell proliferation and target of the Polycomb Repressive Complex 2 (PRC2). We further found that high PCAT-1 and PRC2 expression stratified patient tissues into molecular subtypes distinguished by expression signatures of PCAT-1-repressed target genes. Taken together, the findings presented herein identify PCAT-1 as a novel transcriptional repressor implicated in subset of prostate cancer patients. These findings establish the utility of RNA-Seq to identify disease-associated ncRNAs that may improve the stratification of cancer subtypes. |
format | Online Article Text |
id | pubmed-3152676 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
record_format | MEDLINE/PubMed |
spelling | pubmed-31526762012-02-01 Transcriptome Sequencing Identifies PCAT-1, a Novel lincRNA Implicated in Prostate Cancer Progression Prensner, John R. Iyer, Matthew K. Balbin, O. Alejandro Dhanasekaran, Saravana M. Cao, Qi Brenner, J. Chad Laxman, Bharathi Asangani, Irfan Grasso, Catherine Kominsky, Hal D. Cao, Xuhong Jing, Xiaojun Wang, Xiaoju Siddiqui, Javed Wei, John T. Robinson, Daniel Iyer, Hari K. Palanisamy, Nallasivam Maher, Christopher A. Chinnaiyan, Arul M. Nat Biotechnol Article High-throughput sequencing of polyA+ RNA (RNA-Seq) in human cancer shows remarkable potential to identify both novel markers of disease and uncharacterized aspects of tumor biology, particularly non-coding RNA (ncRNA) species. We employed RNA-Seq on a cohort of 102 prostate tissues and cells lines and performed ab initio transcriptome assembly to discover unannotated ncRNAs. We nominated 121 such Prostate Cancer Associated Transcripts (PCATs) with cancer-specific expression patterns. Among these, we characterized PCAT-1 as a novel prostate-specific regulator of cell proliferation and target of the Polycomb Repressive Complex 2 (PRC2). We further found that high PCAT-1 and PRC2 expression stratified patient tissues into molecular subtypes distinguished by expression signatures of PCAT-1-repressed target genes. Taken together, the findings presented herein identify PCAT-1 as a novel transcriptional repressor implicated in subset of prostate cancer patients. These findings establish the utility of RNA-Seq to identify disease-associated ncRNAs that may improve the stratification of cancer subtypes. 2011-07-31 /pmc/articles/PMC3152676/ /pubmed/21804560 http://dx.doi.org/10.1038/nbt.1914 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Prensner, John R. Iyer, Matthew K. Balbin, O. Alejandro Dhanasekaran, Saravana M. Cao, Qi Brenner, J. Chad Laxman, Bharathi Asangani, Irfan Grasso, Catherine Kominsky, Hal D. Cao, Xuhong Jing, Xiaojun Wang, Xiaoju Siddiqui, Javed Wei, John T. Robinson, Daniel Iyer, Hari K. Palanisamy, Nallasivam Maher, Christopher A. Chinnaiyan, Arul M. Transcriptome Sequencing Identifies PCAT-1, a Novel lincRNA Implicated in Prostate Cancer Progression |
title | Transcriptome Sequencing Identifies PCAT-1, a Novel lincRNA Implicated in Prostate Cancer Progression |
title_full | Transcriptome Sequencing Identifies PCAT-1, a Novel lincRNA Implicated in Prostate Cancer Progression |
title_fullStr | Transcriptome Sequencing Identifies PCAT-1, a Novel lincRNA Implicated in Prostate Cancer Progression |
title_full_unstemmed | Transcriptome Sequencing Identifies PCAT-1, a Novel lincRNA Implicated in Prostate Cancer Progression |
title_short | Transcriptome Sequencing Identifies PCAT-1, a Novel lincRNA Implicated in Prostate Cancer Progression |
title_sort | transcriptome sequencing identifies pcat-1, a novel lincrna implicated in prostate cancer progression |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3152676/ https://www.ncbi.nlm.nih.gov/pubmed/21804560 http://dx.doi.org/10.1038/nbt.1914 |
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