An Updated Loop-Mediated Isothermal Amplification Method for Rapid Diagnosis of H5N1 Avian Influenza Viruses

We designed a new set of primers for reverse transcriptase loop-mediated isothermal amplification (RTLAMP) to specifically amplify the HA gene of avian influenza viruses subtype H5N1. By testing nine H5N1 virus strains and 41 clinical samples collected in Northern Vietnam, we found that the new prim...

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Autores principales: Dinh, Duc Tuan, Le, Mai Thi Quynh, Vuong, Cuong Duc, Hasebe, Futoshi, Morita, Kouichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Tropical Medicine 2011
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3153145/
https://www.ncbi.nlm.nih.gov/pubmed/22028606
http://dx.doi.org/10.2149/tmh.2010-21
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author Dinh, Duc Tuan
Le, Mai Thi Quynh
Vuong, Cuong Duc
Hasebe, Futoshi
Morita, Kouichi
author_facet Dinh, Duc Tuan
Le, Mai Thi Quynh
Vuong, Cuong Duc
Hasebe, Futoshi
Morita, Kouichi
author_sort Dinh, Duc Tuan
collection PubMed
description We designed a new set of primers for reverse transcriptase loop-mediated isothermal amplification (RTLAMP) to specifically amplify the HA gene of avian influenza viruses subtype H5N1. By testing nine H5N1 virus strains and 41 clinical samples collected in Northern Vietnam, we found that the new primers showed higher sensitivity and specificity than the previously published RT-LAMP primers and were comparable to the RT-PCR method currently recommended by WHO. These results suggest that our RT-LAMP assay may be a better choice as a diagnostic tool for current H5N1 influenza virus infection.
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spelling pubmed-31531452011-10-25 An Updated Loop-Mediated Isothermal Amplification Method for Rapid Diagnosis of H5N1 Avian Influenza Viruses Dinh, Duc Tuan Le, Mai Thi Quynh Vuong, Cuong Duc Hasebe, Futoshi Morita, Kouichi Trop Med Health Original Article We designed a new set of primers for reverse transcriptase loop-mediated isothermal amplification (RTLAMP) to specifically amplify the HA gene of avian influenza viruses subtype H5N1. By testing nine H5N1 virus strains and 41 clinical samples collected in Northern Vietnam, we found that the new primers showed higher sensitivity and specificity than the previously published RT-LAMP primers and were comparable to the RT-PCR method currently recommended by WHO. These results suggest that our RT-LAMP assay may be a better choice as a diagnostic tool for current H5N1 influenza virus infection. The Japanese Society of Tropical Medicine 2011-03 2011-03-24 /pmc/articles/PMC3153145/ /pubmed/22028606 http://dx.doi.org/10.2149/tmh.2010-21 Text en © 2011 Japanese Society of Tropical Medicine This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Dinh, Duc Tuan
Le, Mai Thi Quynh
Vuong, Cuong Duc
Hasebe, Futoshi
Morita, Kouichi
An Updated Loop-Mediated Isothermal Amplification Method for Rapid Diagnosis of H5N1 Avian Influenza Viruses
title An Updated Loop-Mediated Isothermal Amplification Method for Rapid Diagnosis of H5N1 Avian Influenza Viruses
title_full An Updated Loop-Mediated Isothermal Amplification Method for Rapid Diagnosis of H5N1 Avian Influenza Viruses
title_fullStr An Updated Loop-Mediated Isothermal Amplification Method for Rapid Diagnosis of H5N1 Avian Influenza Viruses
title_full_unstemmed An Updated Loop-Mediated Isothermal Amplification Method for Rapid Diagnosis of H5N1 Avian Influenza Viruses
title_short An Updated Loop-Mediated Isothermal Amplification Method for Rapid Diagnosis of H5N1 Avian Influenza Viruses
title_sort updated loop-mediated isothermal amplification method for rapid diagnosis of h5n1 avian influenza viruses
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3153145/
https://www.ncbi.nlm.nih.gov/pubmed/22028606
http://dx.doi.org/10.2149/tmh.2010-21
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