Shiga Toxin Is Transported into the Nucleoli of Intestinal Epithelial Cells via a Carrier-Dependent Process

Shiga toxin (Stx) produced by the invasive Shigella dysenteriae serotype 1 (S. dysenteriae1) causes gastrointestinal and kidney complications. It has been assumed that Stx is released intracellularly after enterocyte invasion by S. dysenteriae1. However, there is little information about Stx distrib...

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Detalles Bibliográficos
Autores principales: Baibakov, Boris, Murtazina, Rakhilya, Elowsky, Christian, Giardiello, Francis M., Kovbasnjuk, Olga
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3153243/
https://www.ncbi.nlm.nih.gov/pubmed/22069640
http://dx.doi.org/10.3390/toxins2061318
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author Baibakov, Boris
Murtazina, Rakhilya
Elowsky, Christian
Giardiello, Francis M.
Kovbasnjuk, Olga
author_facet Baibakov, Boris
Murtazina, Rakhilya
Elowsky, Christian
Giardiello, Francis M.
Kovbasnjuk, Olga
author_sort Baibakov, Boris
collection PubMed
description Shiga toxin (Stx) produced by the invasive Shigella dysenteriae serotype 1 (S. dysenteriae1) causes gastrointestinal and kidney complications. It has been assumed that Stx is released intracellularly after enterocyte invasion by S. dysenteriae1. However, there is little information about Stx distribution inside S. dysenteriae1-infected enterocytes. Here, we use intestinal epithelial T84 cells to characterize the trafficking of Stx delivered into the cytosol, in ways that mimic aspects of S. dysenteriae1 infection. We find that cytoplasmic Stx is transported into nucleoli. Stx nucleolar movement is carrier- and energy-dependent. Stx binding to the nucleoli of normal human enterocytes in vitro supports possible roles for nucleolar trafficking in toxin-induced intestinal pathology.
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spelling pubmed-31532432011-11-08 Shiga Toxin Is Transported into the Nucleoli of Intestinal Epithelial Cells via a Carrier-Dependent Process Baibakov, Boris Murtazina, Rakhilya Elowsky, Christian Giardiello, Francis M. Kovbasnjuk, Olga Toxins (Basel) Article Shiga toxin (Stx) produced by the invasive Shigella dysenteriae serotype 1 (S. dysenteriae1) causes gastrointestinal and kidney complications. It has been assumed that Stx is released intracellularly after enterocyte invasion by S. dysenteriae1. However, there is little information about Stx distribution inside S. dysenteriae1-infected enterocytes. Here, we use intestinal epithelial T84 cells to characterize the trafficking of Stx delivered into the cytosol, in ways that mimic aspects of S. dysenteriae1 infection. We find that cytoplasmic Stx is transported into nucleoli. Stx nucleolar movement is carrier- and energy-dependent. Stx binding to the nucleoli of normal human enterocytes in vitro supports possible roles for nucleolar trafficking in toxin-induced intestinal pathology. MDPI 2010-06-07 /pmc/articles/PMC3153243/ /pubmed/22069640 http://dx.doi.org/10.3390/toxins2061318 Text en © 2010 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Baibakov, Boris
Murtazina, Rakhilya
Elowsky, Christian
Giardiello, Francis M.
Kovbasnjuk, Olga
Shiga Toxin Is Transported into the Nucleoli of Intestinal Epithelial Cells via a Carrier-Dependent Process
title Shiga Toxin Is Transported into the Nucleoli of Intestinal Epithelial Cells via a Carrier-Dependent Process
title_full Shiga Toxin Is Transported into the Nucleoli of Intestinal Epithelial Cells via a Carrier-Dependent Process
title_fullStr Shiga Toxin Is Transported into the Nucleoli of Intestinal Epithelial Cells via a Carrier-Dependent Process
title_full_unstemmed Shiga Toxin Is Transported into the Nucleoli of Intestinal Epithelial Cells via a Carrier-Dependent Process
title_short Shiga Toxin Is Transported into the Nucleoli of Intestinal Epithelial Cells via a Carrier-Dependent Process
title_sort shiga toxin is transported into the nucleoli of intestinal epithelial cells via a carrier-dependent process
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3153243/
https://www.ncbi.nlm.nih.gov/pubmed/22069640
http://dx.doi.org/10.3390/toxins2061318
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