Development and Characterization of a Monoclonal Antibody against Ochratoxin B and Its Application in ELISA

A monoclonal antibody specific to ochratoxin B (OTB) was employed for the development of an indirect competitive OTB-ELISA. The optimized OTB-ELISA resulted in a limit of detection (LOD) for OTB of 3 µg/L (8 nM), a limit of quantification (LOQ) of 3.7 µg/L (10 nM), and a 50% inhibitory concentration...

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Detalles Bibliográficos
Autores principales: Heussner, Alexandra H., Ausländer, Simon, Dietrich, Daniel R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3153249/
https://www.ncbi.nlm.nih.gov/pubmed/22069651
http://dx.doi.org/10.3390/toxins2061582
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author Heussner, Alexandra H.
Ausländer, Simon
Dietrich, Daniel R.
author_facet Heussner, Alexandra H.
Ausländer, Simon
Dietrich, Daniel R.
author_sort Heussner, Alexandra H.
collection PubMed
description A monoclonal antibody specific to ochratoxin B (OTB) was employed for the development of an indirect competitive OTB-ELISA. The optimized OTB-ELISA resulted in a limit of detection (LOD) for OTB of 3 µg/L (8 nM), a limit of quantification (LOQ) of 3.7 µg/L (10 nM), and a 50% inhibitory concentration (IC(50)) of 150 nM. Due to very low cross-reactivity to OTA (2.7%) and structurally related molecules (0%), this OTB-ELISA was found to be suitable to detect OTB with excellent precision in different matrices, i.e., beer, coffee and wine. Therefore, this OTB-ELISA will allow screening of OTB in food and feed products.
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spelling pubmed-31532492011-11-08 Development and Characterization of a Monoclonal Antibody against Ochratoxin B and Its Application in ELISA Heussner, Alexandra H. Ausländer, Simon Dietrich, Daniel R. Toxins (Basel) Article A monoclonal antibody specific to ochratoxin B (OTB) was employed for the development of an indirect competitive OTB-ELISA. The optimized OTB-ELISA resulted in a limit of detection (LOD) for OTB of 3 µg/L (8 nM), a limit of quantification (LOQ) of 3.7 µg/L (10 nM), and a 50% inhibitory concentration (IC(50)) of 150 nM. Due to very low cross-reactivity to OTA (2.7%) and structurally related molecules (0%), this OTB-ELISA was found to be suitable to detect OTB with excellent precision in different matrices, i.e., beer, coffee and wine. Therefore, this OTB-ELISA will allow screening of OTB in food and feed products. MDPI 2010-06-21 /pmc/articles/PMC3153249/ /pubmed/22069651 http://dx.doi.org/10.3390/toxins2061582 Text en © 2010 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Heussner, Alexandra H.
Ausländer, Simon
Dietrich, Daniel R.
Development and Characterization of a Monoclonal Antibody against Ochratoxin B and Its Application in ELISA
title Development and Characterization of a Monoclonal Antibody against Ochratoxin B and Its Application in ELISA
title_full Development and Characterization of a Monoclonal Antibody against Ochratoxin B and Its Application in ELISA
title_fullStr Development and Characterization of a Monoclonal Antibody against Ochratoxin B and Its Application in ELISA
title_full_unstemmed Development and Characterization of a Monoclonal Antibody against Ochratoxin B and Its Application in ELISA
title_short Development and Characterization of a Monoclonal Antibody against Ochratoxin B and Its Application in ELISA
title_sort development and characterization of a monoclonal antibody against ochratoxin b and its application in elisa
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3153249/
https://www.ncbi.nlm.nih.gov/pubmed/22069651
http://dx.doi.org/10.3390/toxins2061582
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