Alpha-Tocopherol Counteracts the Cytotoxicity Induced by Ochratoxin A in Primary Porcine Fibroblasts

The aims of the current study were to determine the half-lethal concentration of ochratoxin A (OTA) as well as the levels of lactate dehydrogenase release and DNA fragmentation induced by OTA in primary porcine fibroblasts, and to examine the role of α-tocopherol in counteracting its toxicity. Cells...

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Autores principales: Fusi, Eleonora, Rebucci, Raffaella, Pecorini, Chiara, Campagnoli, Anna, Pinotti, Luciano, Saccone, Francesca, Cheli, Federica, Purup, Stig, Sejrsen, Kristen, Baldi, Antonella
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2010
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3153250/
https://www.ncbi.nlm.nih.gov/pubmed/22069637
http://dx.doi.org/10.3390/toxins2061265
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author Fusi, Eleonora
Rebucci, Raffaella
Pecorini, Chiara
Campagnoli, Anna
Pinotti, Luciano
Saccone, Francesca
Cheli, Federica
Purup, Stig
Sejrsen, Kristen
Baldi, Antonella
author_facet Fusi, Eleonora
Rebucci, Raffaella
Pecorini, Chiara
Campagnoli, Anna
Pinotti, Luciano
Saccone, Francesca
Cheli, Federica
Purup, Stig
Sejrsen, Kristen
Baldi, Antonella
author_sort Fusi, Eleonora
collection PubMed
description The aims of the current study were to determine the half-lethal concentration of ochratoxin A (OTA) as well as the levels of lactate dehydrogenase release and DNA fragmentation induced by OTA in primary porcine fibroblasts, and to examine the role of α-tocopherol in counteracting its toxicity. Cells showed a dose-, time- and origin-dependent (ear vs. embryo) sensitivity to ochratoxin A. Pre-incubation for 3 h with 1 nM α-tocopherol significantly (P < 0.01) reduced OTA cytotoxicity, lactate dehydrogenase release and DNA damage in both fibroblast cultures. These findings indicate that α-tocopherol supplementation may counteract short-term OTA toxicity, supporting its defensive role in the cell membrane.
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spelling pubmed-31532502011-11-08 Alpha-Tocopherol Counteracts the Cytotoxicity Induced by Ochratoxin A in Primary Porcine Fibroblasts Fusi, Eleonora Rebucci, Raffaella Pecorini, Chiara Campagnoli, Anna Pinotti, Luciano Saccone, Francesca Cheli, Federica Purup, Stig Sejrsen, Kristen Baldi, Antonella Toxins (Basel) Article The aims of the current study were to determine the half-lethal concentration of ochratoxin A (OTA) as well as the levels of lactate dehydrogenase release and DNA fragmentation induced by OTA in primary porcine fibroblasts, and to examine the role of α-tocopherol in counteracting its toxicity. Cells showed a dose-, time- and origin-dependent (ear vs. embryo) sensitivity to ochratoxin A. Pre-incubation for 3 h with 1 nM α-tocopherol significantly (P < 0.01) reduced OTA cytotoxicity, lactate dehydrogenase release and DNA damage in both fibroblast cultures. These findings indicate that α-tocopherol supplementation may counteract short-term OTA toxicity, supporting its defensive role in the cell membrane. MDPI 2010-06-01 /pmc/articles/PMC3153250/ /pubmed/22069637 http://dx.doi.org/10.3390/toxins2061265 Text en © 2010 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Fusi, Eleonora
Rebucci, Raffaella
Pecorini, Chiara
Campagnoli, Anna
Pinotti, Luciano
Saccone, Francesca
Cheli, Federica
Purup, Stig
Sejrsen, Kristen
Baldi, Antonella
Alpha-Tocopherol Counteracts the Cytotoxicity Induced by Ochratoxin A in Primary Porcine Fibroblasts
title Alpha-Tocopherol Counteracts the Cytotoxicity Induced by Ochratoxin A in Primary Porcine Fibroblasts
title_full Alpha-Tocopherol Counteracts the Cytotoxicity Induced by Ochratoxin A in Primary Porcine Fibroblasts
title_fullStr Alpha-Tocopherol Counteracts the Cytotoxicity Induced by Ochratoxin A in Primary Porcine Fibroblasts
title_full_unstemmed Alpha-Tocopherol Counteracts the Cytotoxicity Induced by Ochratoxin A in Primary Porcine Fibroblasts
title_short Alpha-Tocopherol Counteracts the Cytotoxicity Induced by Ochratoxin A in Primary Porcine Fibroblasts
title_sort alpha-tocopherol counteracts the cytotoxicity induced by ochratoxin a in primary porcine fibroblasts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3153250/
https://www.ncbi.nlm.nih.gov/pubmed/22069637
http://dx.doi.org/10.3390/toxins2061265
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