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Gene Expression Profiling in Monocytes and SNP Association Suggest the Importance of the Gene for Osteoporosis in Both Chinese and Caucasians
Osteoporosis is characterized mainly by low bone mineral density (BMD). Many cytokines and chemokines have been related with bone metabolism. Monocytes in the immune system are important sources of cytokines and chemokines for bone metabolism. However, no study has investigated in vivo expression of...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wiley Subscription Services, Inc., A Wiley Company
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3153389/ https://www.ncbi.nlm.nih.gov/pubmed/19594299 http://dx.doi.org/10.1359/jbmr.090724 |
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author | Chen, Xiang-Ding Xiao, Peng Lei, Shu-Feng Liu, Yao-Zhong Guo, Yan-Fang Deng, Fei-Yan Tan, Li-Jun Zhu, Xue-Zhen Chen, Fu-Rong Recker, Robert R Deng, Hong-Wen |
author_facet | Chen, Xiang-Ding Xiao, Peng Lei, Shu-Feng Liu, Yao-Zhong Guo, Yan-Fang Deng, Fei-Yan Tan, Li-Jun Zhu, Xue-Zhen Chen, Fu-Rong Recker, Robert R Deng, Hong-Wen |
author_sort | Chen, Xiang-Ding |
collection | PubMed |
description | Osteoporosis is characterized mainly by low bone mineral density (BMD). Many cytokines and chemokines have been related with bone metabolism. Monocytes in the immune system are important sources of cytokines and chemokines for bone metabolism. However, no study has investigated in vivo expression of a large number of various factors simultaneously in human monocytes underlying osteoporosis. This study explored the in vivo expression pattern of general cytokines, chemokines, and their receptor genes in human monocytes and validated the significant genes by qRT-PCR and genetic association analyses. Expression profilings were performed in monocyte samples from 26 Chinese and 20 Caucasian premenopausal women with discordant BMD. Genome-wide association analysis with BMD variation was conducted in 1000 unrelated Caucasians. We selected 168 cytokines, chemokines, osteoclast-related factors, and their receptor genes for analyses. Significantly, the signal transducer and activator of transcription 1 (STAT1) gene was upregulated in the low versus the high BMD groups in both Chinese and Caucasians. We also revealed a significant association of the STAT1 gene with BMD variation in the 1000 Caucasians. Thus we conclude that the STAT1 gene is important in human circulating monocytes in the etiology of osteoporosis. © 2010 American Society for Bone and Mineral Research. |
format | Online Article Text |
id | pubmed-3153389 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Wiley Subscription Services, Inc., A Wiley Company |
record_format | MEDLINE/PubMed |
spelling | pubmed-31533892011-08-19 Gene Expression Profiling in Monocytes and SNP Association Suggest the Importance of the Gene for Osteoporosis in Both Chinese and Caucasians Chen, Xiang-Ding Xiao, Peng Lei, Shu-Feng Liu, Yao-Zhong Guo, Yan-Fang Deng, Fei-Yan Tan, Li-Jun Zhu, Xue-Zhen Chen, Fu-Rong Recker, Robert R Deng, Hong-Wen J Bone Miner Res Original Article Osteoporosis is characterized mainly by low bone mineral density (BMD). Many cytokines and chemokines have been related with bone metabolism. Monocytes in the immune system are important sources of cytokines and chemokines for bone metabolism. However, no study has investigated in vivo expression of a large number of various factors simultaneously in human monocytes underlying osteoporosis. This study explored the in vivo expression pattern of general cytokines, chemokines, and their receptor genes in human monocytes and validated the significant genes by qRT-PCR and genetic association analyses. Expression profilings were performed in monocyte samples from 26 Chinese and 20 Caucasian premenopausal women with discordant BMD. Genome-wide association analysis with BMD variation was conducted in 1000 unrelated Caucasians. We selected 168 cytokines, chemokines, osteoclast-related factors, and their receptor genes for analyses. Significantly, the signal transducer and activator of transcription 1 (STAT1) gene was upregulated in the low versus the high BMD groups in both Chinese and Caucasians. We also revealed a significant association of the STAT1 gene with BMD variation in the 1000 Caucasians. Thus we conclude that the STAT1 gene is important in human circulating monocytes in the etiology of osteoporosis. © 2010 American Society for Bone and Mineral Research. Wiley Subscription Services, Inc., A Wiley Company 2010-02 2009-07-13 /pmc/articles/PMC3153389/ /pubmed/19594299 http://dx.doi.org/10.1359/jbmr.090724 Text en Copyright © 2010 American Society for Bone and Mineral Research http://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation. |
spellingShingle | Original Article Chen, Xiang-Ding Xiao, Peng Lei, Shu-Feng Liu, Yao-Zhong Guo, Yan-Fang Deng, Fei-Yan Tan, Li-Jun Zhu, Xue-Zhen Chen, Fu-Rong Recker, Robert R Deng, Hong-Wen Gene Expression Profiling in Monocytes and SNP Association Suggest the Importance of the Gene for Osteoporosis in Both Chinese and Caucasians |
title | Gene Expression Profiling in Monocytes and SNP Association Suggest the Importance of the Gene for Osteoporosis in Both Chinese and Caucasians |
title_full | Gene Expression Profiling in Monocytes and SNP Association Suggest the Importance of the Gene for Osteoporosis in Both Chinese and Caucasians |
title_fullStr | Gene Expression Profiling in Monocytes and SNP Association Suggest the Importance of the Gene for Osteoporosis in Both Chinese and Caucasians |
title_full_unstemmed | Gene Expression Profiling in Monocytes and SNP Association Suggest the Importance of the Gene for Osteoporosis in Both Chinese and Caucasians |
title_short | Gene Expression Profiling in Monocytes and SNP Association Suggest the Importance of the Gene for Osteoporosis in Both Chinese and Caucasians |
title_sort | gene expression profiling in monocytes and snp association suggest the importance of the gene for osteoporosis in both chinese and caucasians |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3153389/ https://www.ncbi.nlm.nih.gov/pubmed/19594299 http://dx.doi.org/10.1359/jbmr.090724 |
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