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Protein and Amino Acid Supplementation Does Not Alter Proteolytic Gene Expression following Immobilization
Objective. To determine if supplementation of protein and amino acids (PAA) decreases skeletal muscle expression of atrophy-related genes, muscle mass, and strength during immobilization in humans. Methods. Twenty males wore a lower-limb immobilization boot for 28 days and consumed either a PAA supp...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3153915/ https://www.ncbi.nlm.nih.gov/pubmed/21845220 http://dx.doi.org/10.1155/2011/539690 |
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author | Bunn, Jennifer A. Buford, Thomas W. Serra, Monica C. Kreider, Richard B. Willoughby, Darryn S. |
author_facet | Bunn, Jennifer A. Buford, Thomas W. Serra, Monica C. Kreider, Richard B. Willoughby, Darryn S. |
author_sort | Bunn, Jennifer A. |
collection | PubMed |
description | Objective. To determine if supplementation of protein and amino acids (PAA) decreases skeletal muscle expression of atrophy-related genes, muscle mass, and strength during immobilization in humans. Methods. Twenty males wore a lower-limb immobilization boot for 28 days and consumed either a PAA supplement (28 g protein) or carbohydrate placebo (28 g maltodextrose), while consuming their normal daily diet. Testing sessions included dietary analysis, lower-leg girth and body composition measurements, strength testing, and gastrocnemius muscle biopsies. Muscle was analyzed for mRNA expression of markers in the ubiquitin and calpain systems, myostatin, TNF-α, and NF-κB. Results. All genes of interest increased over time (P < .05), but there was no difference between groups. Lower-leg girth decreased over time (P = 0.02); however, there were no significant changes in body composition or strength. Conclusion. Short-term lower-limb disuse, despite the absence of significant muscle atrophy, is associated with increases in skeletal muscle gene expression of several proteolysis-related genes. These changes do not appear to be altered by oral PAA supplementation. |
format | Online Article Text |
id | pubmed-3153915 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-31539152011-08-15 Protein and Amino Acid Supplementation Does Not Alter Proteolytic Gene Expression following Immobilization Bunn, Jennifer A. Buford, Thomas W. Serra, Monica C. Kreider, Richard B. Willoughby, Darryn S. J Nutr Metab Research Article Objective. To determine if supplementation of protein and amino acids (PAA) decreases skeletal muscle expression of atrophy-related genes, muscle mass, and strength during immobilization in humans. Methods. Twenty males wore a lower-limb immobilization boot for 28 days and consumed either a PAA supplement (28 g protein) or carbohydrate placebo (28 g maltodextrose), while consuming their normal daily diet. Testing sessions included dietary analysis, lower-leg girth and body composition measurements, strength testing, and gastrocnemius muscle biopsies. Muscle was analyzed for mRNA expression of markers in the ubiquitin and calpain systems, myostatin, TNF-α, and NF-κB. Results. All genes of interest increased over time (P < .05), but there was no difference between groups. Lower-leg girth decreased over time (P = 0.02); however, there were no significant changes in body composition or strength. Conclusion. Short-term lower-limb disuse, despite the absence of significant muscle atrophy, is associated with increases in skeletal muscle gene expression of several proteolysis-related genes. These changes do not appear to be altered by oral PAA supplementation. Hindawi Publishing Corporation 2011 2011-08-09 /pmc/articles/PMC3153915/ /pubmed/21845220 http://dx.doi.org/10.1155/2011/539690 Text en Copyright © 2011 Jennifer A. Bunn et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Bunn, Jennifer A. Buford, Thomas W. Serra, Monica C. Kreider, Richard B. Willoughby, Darryn S. Protein and Amino Acid Supplementation Does Not Alter Proteolytic Gene Expression following Immobilization |
title | Protein and Amino Acid Supplementation Does Not Alter Proteolytic Gene Expression following Immobilization |
title_full | Protein and Amino Acid Supplementation Does Not Alter Proteolytic Gene Expression following Immobilization |
title_fullStr | Protein and Amino Acid Supplementation Does Not Alter Proteolytic Gene Expression following Immobilization |
title_full_unstemmed | Protein and Amino Acid Supplementation Does Not Alter Proteolytic Gene Expression following Immobilization |
title_short | Protein and Amino Acid Supplementation Does Not Alter Proteolytic Gene Expression following Immobilization |
title_sort | protein and amino acid supplementation does not alter proteolytic gene expression following immobilization |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3153915/ https://www.ncbi.nlm.nih.gov/pubmed/21845220 http://dx.doi.org/10.1155/2011/539690 |
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