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Protein and Amino Acid Supplementation Does Not Alter Proteolytic Gene Expression following Immobilization

Objective. To determine if supplementation of protein and amino acids (PAA) decreases skeletal muscle expression of atrophy-related genes, muscle mass, and strength during immobilization in humans. Methods. Twenty males wore a lower-limb immobilization boot for 28 days and consumed either a PAA supp...

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Autores principales: Bunn, Jennifer A., Buford, Thomas W., Serra, Monica C., Kreider, Richard B., Willoughby, Darryn S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3153915/
https://www.ncbi.nlm.nih.gov/pubmed/21845220
http://dx.doi.org/10.1155/2011/539690
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author Bunn, Jennifer A.
Buford, Thomas W.
Serra, Monica C.
Kreider, Richard B.
Willoughby, Darryn S.
author_facet Bunn, Jennifer A.
Buford, Thomas W.
Serra, Monica C.
Kreider, Richard B.
Willoughby, Darryn S.
author_sort Bunn, Jennifer A.
collection PubMed
description Objective. To determine if supplementation of protein and amino acids (PAA) decreases skeletal muscle expression of atrophy-related genes, muscle mass, and strength during immobilization in humans. Methods. Twenty males wore a lower-limb immobilization boot for 28 days and consumed either a PAA supplement (28 g protein) or carbohydrate placebo (28 g maltodextrose), while consuming their normal daily diet. Testing sessions included dietary analysis, lower-leg girth and body composition measurements, strength testing, and gastrocnemius muscle biopsies. Muscle was analyzed for mRNA expression of markers in the ubiquitin and calpain systems, myostatin, TNF-α, and NF-κB. Results. All genes of interest increased over time (P < .05), but there was no difference between groups. Lower-leg girth decreased over time (P = 0.02); however, there were no significant changes in body composition or strength. Conclusion. Short-term lower-limb disuse, despite the absence of significant muscle atrophy, is associated with increases in skeletal muscle gene expression of several proteolysis-related genes. These changes do not appear to be altered by oral PAA supplementation.
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spelling pubmed-31539152011-08-15 Protein and Amino Acid Supplementation Does Not Alter Proteolytic Gene Expression following Immobilization Bunn, Jennifer A. Buford, Thomas W. Serra, Monica C. Kreider, Richard B. Willoughby, Darryn S. J Nutr Metab Research Article Objective. To determine if supplementation of protein and amino acids (PAA) decreases skeletal muscle expression of atrophy-related genes, muscle mass, and strength during immobilization in humans. Methods. Twenty males wore a lower-limb immobilization boot for 28 days and consumed either a PAA supplement (28 g protein) or carbohydrate placebo (28 g maltodextrose), while consuming their normal daily diet. Testing sessions included dietary analysis, lower-leg girth and body composition measurements, strength testing, and gastrocnemius muscle biopsies. Muscle was analyzed for mRNA expression of markers in the ubiquitin and calpain systems, myostatin, TNF-α, and NF-κB. Results. All genes of interest increased over time (P < .05), but there was no difference between groups. Lower-leg girth decreased over time (P = 0.02); however, there were no significant changes in body composition or strength. Conclusion. Short-term lower-limb disuse, despite the absence of significant muscle atrophy, is associated with increases in skeletal muscle gene expression of several proteolysis-related genes. These changes do not appear to be altered by oral PAA supplementation. Hindawi Publishing Corporation 2011 2011-08-09 /pmc/articles/PMC3153915/ /pubmed/21845220 http://dx.doi.org/10.1155/2011/539690 Text en Copyright © 2011 Jennifer A. Bunn et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Bunn, Jennifer A.
Buford, Thomas W.
Serra, Monica C.
Kreider, Richard B.
Willoughby, Darryn S.
Protein and Amino Acid Supplementation Does Not Alter Proteolytic Gene Expression following Immobilization
title Protein and Amino Acid Supplementation Does Not Alter Proteolytic Gene Expression following Immobilization
title_full Protein and Amino Acid Supplementation Does Not Alter Proteolytic Gene Expression following Immobilization
title_fullStr Protein and Amino Acid Supplementation Does Not Alter Proteolytic Gene Expression following Immobilization
title_full_unstemmed Protein and Amino Acid Supplementation Does Not Alter Proteolytic Gene Expression following Immobilization
title_short Protein and Amino Acid Supplementation Does Not Alter Proteolytic Gene Expression following Immobilization
title_sort protein and amino acid supplementation does not alter proteolytic gene expression following immobilization
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3153915/
https://www.ncbi.nlm.nih.gov/pubmed/21845220
http://dx.doi.org/10.1155/2011/539690
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