Cargando…
Origin Matters: Differences in Embryonic Tissue Origin and Wnt Signaling Determine the Osteogenic Potential and Healing Capacity of Frontal and Parietal Calvarial Bones
Calvarial bones arise from two embryonic tissues, namely, the neural crest and the mesoderm. In this study we have addressed the important question of whether disparate embryonic tissue origins impart variable osteogenic potential and regenerative capacity to calvarial bones, as well as what the und...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wiley Subscription Services, Inc., A Wiley Company
2010
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3154006/ https://www.ncbi.nlm.nih.gov/pubmed/19929441 http://dx.doi.org/10.1359/jbmr.091116 |
_version_ | 1782209971324190720 |
---|---|
author | Quarto, Natalina Wan, Derrick C Kwan, Matt D Panetta, Nicholas J Li, Shuli Longaker, Michael T |
author_facet | Quarto, Natalina Wan, Derrick C Kwan, Matt D Panetta, Nicholas J Li, Shuli Longaker, Michael T |
author_sort | Quarto, Natalina |
collection | PubMed |
description | Calvarial bones arise from two embryonic tissues, namely, the neural crest and the mesoderm. In this study we have addressed the important question of whether disparate embryonic tissue origins impart variable osteogenic potential and regenerative capacity to calvarial bones, as well as what the underlying molecular mechanism(s). Thus, by performing in vitro and in vivo studies, we have investigated whether differences exist between neural crest–derived frontal and paraxial mesodermal–derived parietal bone. Of interest, our data indicate that calvarial bone osteoblasts of neural crest origin have superior potential for osteogenic differentiation. Furthermore, neural crest–derived frontal bone displays a superior capacity to undergo osseous healing compared with calvarial bone of paraxial mesoderm origin. Our study identified both in vitro and in vivo enhanced endogenous canonical Wnt signaling in frontal bone compared with parietal bone. In addition, we demonstrate that constitutive activation of canonical Wnt signaling in paraxial mesodermal–derived parietal osteoblasts mimics the osteogenic potential of frontal osteoblasts, whereas knockdown of canonical Wnt signaling dramatically impairs the greater osteogenic potential of neural crest–derived frontal osteoblasts. Moreover, fibroblast growth factor 2 (FGF-2) treatment induces phosphorylation of GSK-3β and increases the nuclear levels of β-catenin in osteoblasts, suggesting that enhanced activation of Wnt signaling might be mediated by FGF. Taken together, our data provide compelling evidence that indeed embryonic tissue origin makes a difference and that active canonical Wnt signaling plays a major role in contributing to the superior intrinsic osteogenic potential and tissue regeneration observed in neural crest–derived frontal bone. © 2010 American Society for Bone and Mineral Research. |
format | Online Article Text |
id | pubmed-3154006 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Wiley Subscription Services, Inc., A Wiley Company |
record_format | MEDLINE/PubMed |
spelling | pubmed-31540062011-08-19 Origin Matters: Differences in Embryonic Tissue Origin and Wnt Signaling Determine the Osteogenic Potential and Healing Capacity of Frontal and Parietal Calvarial Bones Quarto, Natalina Wan, Derrick C Kwan, Matt D Panetta, Nicholas J Li, Shuli Longaker, Michael T J Bone Miner Res Original Article Calvarial bones arise from two embryonic tissues, namely, the neural crest and the mesoderm. In this study we have addressed the important question of whether disparate embryonic tissue origins impart variable osteogenic potential and regenerative capacity to calvarial bones, as well as what the underlying molecular mechanism(s). Thus, by performing in vitro and in vivo studies, we have investigated whether differences exist between neural crest–derived frontal and paraxial mesodermal–derived parietal bone. Of interest, our data indicate that calvarial bone osteoblasts of neural crest origin have superior potential for osteogenic differentiation. Furthermore, neural crest–derived frontal bone displays a superior capacity to undergo osseous healing compared with calvarial bone of paraxial mesoderm origin. Our study identified both in vitro and in vivo enhanced endogenous canonical Wnt signaling in frontal bone compared with parietal bone. In addition, we demonstrate that constitutive activation of canonical Wnt signaling in paraxial mesodermal–derived parietal osteoblasts mimics the osteogenic potential of frontal osteoblasts, whereas knockdown of canonical Wnt signaling dramatically impairs the greater osteogenic potential of neural crest–derived frontal osteoblasts. Moreover, fibroblast growth factor 2 (FGF-2) treatment induces phosphorylation of GSK-3β and increases the nuclear levels of β-catenin in osteoblasts, suggesting that enhanced activation of Wnt signaling might be mediated by FGF. Taken together, our data provide compelling evidence that indeed embryonic tissue origin makes a difference and that active canonical Wnt signaling plays a major role in contributing to the superior intrinsic osteogenic potential and tissue regeneration observed in neural crest–derived frontal bone. © 2010 American Society for Bone and Mineral Research. Wiley Subscription Services, Inc., A Wiley Company 2010-07 2009-11-23 /pmc/articles/PMC3154006/ /pubmed/19929441 http://dx.doi.org/10.1359/jbmr.091116 Text en Copyright © 2010 American Society for Bone and Mineral Research http://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation. |
spellingShingle | Original Article Quarto, Natalina Wan, Derrick C Kwan, Matt D Panetta, Nicholas J Li, Shuli Longaker, Michael T Origin Matters: Differences in Embryonic Tissue Origin and Wnt Signaling Determine the Osteogenic Potential and Healing Capacity of Frontal and Parietal Calvarial Bones |
title | Origin Matters: Differences in Embryonic Tissue Origin and Wnt Signaling Determine the Osteogenic Potential and Healing Capacity of Frontal and Parietal Calvarial Bones |
title_full | Origin Matters: Differences in Embryonic Tissue Origin and Wnt Signaling Determine the Osteogenic Potential and Healing Capacity of Frontal and Parietal Calvarial Bones |
title_fullStr | Origin Matters: Differences in Embryonic Tissue Origin and Wnt Signaling Determine the Osteogenic Potential and Healing Capacity of Frontal and Parietal Calvarial Bones |
title_full_unstemmed | Origin Matters: Differences in Embryonic Tissue Origin and Wnt Signaling Determine the Osteogenic Potential and Healing Capacity of Frontal and Parietal Calvarial Bones |
title_short | Origin Matters: Differences in Embryonic Tissue Origin and Wnt Signaling Determine the Osteogenic Potential and Healing Capacity of Frontal and Parietal Calvarial Bones |
title_sort | origin matters: differences in embryonic tissue origin and wnt signaling determine the osteogenic potential and healing capacity of frontal and parietal calvarial bones |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3154006/ https://www.ncbi.nlm.nih.gov/pubmed/19929441 http://dx.doi.org/10.1359/jbmr.091116 |
work_keys_str_mv | AT quartonatalina originmattersdifferencesinembryonictissueoriginandwntsignalingdeterminetheosteogenicpotentialandhealingcapacityoffrontalandparietalcalvarialbones AT wanderrickc originmattersdifferencesinembryonictissueoriginandwntsignalingdeterminetheosteogenicpotentialandhealingcapacityoffrontalandparietalcalvarialbones AT kwanmattd originmattersdifferencesinembryonictissueoriginandwntsignalingdeterminetheosteogenicpotentialandhealingcapacityoffrontalandparietalcalvarialbones AT panettanicholasj originmattersdifferencesinembryonictissueoriginandwntsignalingdeterminetheosteogenicpotentialandhealingcapacityoffrontalandparietalcalvarialbones AT lishuli originmattersdifferencesinembryonictissueoriginandwntsignalingdeterminetheosteogenicpotentialandhealingcapacityoffrontalandparietalcalvarialbones AT longakermichaelt originmattersdifferencesinembryonictissueoriginandwntsignalingdeterminetheosteogenicpotentialandhealingcapacityoffrontalandparietalcalvarialbones |