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Involvement of the Rho–mDia1 pathway in the regulation of Golgi complex architecture and dynamics
In mammalian cells, the Golgi apparatus is a ribbon-like, compact structure composed of multiple membrane stacks connected by tubular bridges. Microtubules are known to be important to Golgi integrity, but the role of the actin cytoskeleton in the maintenance of Golgi architecture remains unclear. H...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3154885/ https://www.ncbi.nlm.nih.gov/pubmed/21680709 http://dx.doi.org/10.1091/mbc.E11-01-0007 |
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author | Zilberman, Yuliya Alieva, Naila O. Miserey-Lenkei, Stéphanie Lichtenstein, Alexandra Kam, Zvi Sabanay, Helena Bershadsky, Alexander |
author_facet | Zilberman, Yuliya Alieva, Naila O. Miserey-Lenkei, Stéphanie Lichtenstein, Alexandra Kam, Zvi Sabanay, Helena Bershadsky, Alexander |
author_sort | Zilberman, Yuliya |
collection | PubMed |
description | In mammalian cells, the Golgi apparatus is a ribbon-like, compact structure composed of multiple membrane stacks connected by tubular bridges. Microtubules are known to be important to Golgi integrity, but the role of the actin cytoskeleton in the maintenance of Golgi architecture remains unclear. Here we show that an increase in Rho activity, either by treatment of cells with lysophosphatidic acid or by expression of constitutively active mutants, resulted in pronounced fragmentation of the Golgi complex into ministacks. Golgi dispersion required the involvement of mDia1 formin, a downstream target of Rho and a potent activator of actin polymerization; moreover, constitutively active mDia1, in and of itself, was sufficient for Golgi dispersion. The dispersion process was accompanied by formation of dynamic F-actin patches in the Golgi area. Experiments with cytoskeletal inhibitors (e.g., latrunculin B, blebbistatin, and Taxol) revealed that actin polymerization, myosin-II–driven contractility, and microtubule-based intracellular movement were all involved in the process of Golgi dispersion induced by Rho–mDia1 activation. Live imaging of Golgi recovery revealed that fusion of the small Golgi stacks into larger compartments was repressed in cells with active mDia1. Furthermore, the formation of Rab6-positive transport vesicles derived from the Golgi complex was enhanced upon activation of the Rho–mDia1 pathway. Transient localization of mDia1 to Rab6-positive vesicles was detected in cells expressing active RhoA. Thus, the Rho–mDia1 pathway is involved in regulation of the Golgi structure, affecting remodeling of Golgi membranes. |
format | Online Article Text |
id | pubmed-3154885 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-31548852011-10-30 Involvement of the Rho–mDia1 pathway in the regulation of Golgi complex architecture and dynamics Zilberman, Yuliya Alieva, Naila O. Miserey-Lenkei, Stéphanie Lichtenstein, Alexandra Kam, Zvi Sabanay, Helena Bershadsky, Alexander Mol Biol Cell Articles In mammalian cells, the Golgi apparatus is a ribbon-like, compact structure composed of multiple membrane stacks connected by tubular bridges. Microtubules are known to be important to Golgi integrity, but the role of the actin cytoskeleton in the maintenance of Golgi architecture remains unclear. Here we show that an increase in Rho activity, either by treatment of cells with lysophosphatidic acid or by expression of constitutively active mutants, resulted in pronounced fragmentation of the Golgi complex into ministacks. Golgi dispersion required the involvement of mDia1 formin, a downstream target of Rho and a potent activator of actin polymerization; moreover, constitutively active mDia1, in and of itself, was sufficient for Golgi dispersion. The dispersion process was accompanied by formation of dynamic F-actin patches in the Golgi area. Experiments with cytoskeletal inhibitors (e.g., latrunculin B, blebbistatin, and Taxol) revealed that actin polymerization, myosin-II–driven contractility, and microtubule-based intracellular movement were all involved in the process of Golgi dispersion induced by Rho–mDia1 activation. Live imaging of Golgi recovery revealed that fusion of the small Golgi stacks into larger compartments was repressed in cells with active mDia1. Furthermore, the formation of Rab6-positive transport vesicles derived from the Golgi complex was enhanced upon activation of the Rho–mDia1 pathway. Transient localization of mDia1 to Rab6-positive vesicles was detected in cells expressing active RhoA. Thus, the Rho–mDia1 pathway is involved in regulation of the Golgi structure, affecting remodeling of Golgi membranes. The American Society for Cell Biology 2011-08-15 /pmc/articles/PMC3154885/ /pubmed/21680709 http://dx.doi.org/10.1091/mbc.E11-01-0007 Text en © 2011 Zilberman et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology. |
spellingShingle | Articles Zilberman, Yuliya Alieva, Naila O. Miserey-Lenkei, Stéphanie Lichtenstein, Alexandra Kam, Zvi Sabanay, Helena Bershadsky, Alexander Involvement of the Rho–mDia1 pathway in the regulation of Golgi complex architecture and dynamics |
title | Involvement of the Rho–mDia1 pathway in the regulation of Golgi complex architecture and dynamics |
title_full | Involvement of the Rho–mDia1 pathway in the regulation of Golgi complex architecture and dynamics |
title_fullStr | Involvement of the Rho–mDia1 pathway in the regulation of Golgi complex architecture and dynamics |
title_full_unstemmed | Involvement of the Rho–mDia1 pathway in the regulation of Golgi complex architecture and dynamics |
title_short | Involvement of the Rho–mDia1 pathway in the regulation of Golgi complex architecture and dynamics |
title_sort | involvement of the rho–mdia1 pathway in the regulation of golgi complex architecture and dynamics |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3154885/ https://www.ncbi.nlm.nih.gov/pubmed/21680709 http://dx.doi.org/10.1091/mbc.E11-01-0007 |
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