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A Molecular Assay for Sensitive Detection of Pathogen-Specific T-Cells

Here we describe the development and validation of a highly sensitive assay of antigen-specific IFN-γ production using real time quantitative PCR (qPCR) for two reporters - monokine-induced by IFN-γ (MIG) and the IFN-γ inducible protein-10 (IP10). We developed and validated the assay and applied it...

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Detalles Bibliográficos
Autores principales:	Kasprowicz, Victoria O., Mitchell, Jessica E., Chetty, Shivan, Govender, Pamla, Huang, Kuan-Hsiang Gary, Fletcher, Helen A., Webster, Daniel P., Brown, Sebastian, Kasmar, Anne, Millington, Kerry, Day, Cheryl L., Mkhwanazi, Nompumelelo, McClurg, Cheryl, Chonco, Fundisiwe, Lalvani, Ajit, Walker, Bruce D., Ndung'u, Thumbi, Klenerman, Paul
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2011
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3154901/ https://www.ncbi.nlm.nih.gov/pubmed/21853018 http://dx.doi.org/10.1371/journal.pone.0020606

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