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Phosphatase-Coupled Universal Kinase Assay and Kinetics for First-Order-Rate Coupling Reaction
Kinases use adenosine-5′-triphosphate (ATP) as the donor substrate and generate adenosine-5′-diphosphate (ADP) as a product. An ADP-based phosphatase-coupled kinase assay is described here. In this assay, CD39L2, a nucleotidase, is added into a kinase reaction to hydrolyze ADP to AMP and phosphate....
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Public Library of Science
2011
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3154929/ https://www.ncbi.nlm.nih.gov/pubmed/21853082 http://dx.doi.org/10.1371/journal.pone.0023172 |