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The role of renal hypoperfusion in development of renal microcirculatory dysfunction in endotoxemic rats

PURPOSE: To study the role of renal hypoperfusion in development of renal microcirculatory dysfunction in endotoxemic rats. METHODS: Rats were randomized into four groups: a sham group (n = 6), a lipopolysaccharide (LPS) group (n = 6), a group in which LPS administration was followed by immediate fl...

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Autores principales: Legrand, Matthieu, Bezemer, Rick, Kandil, Asli, Demirci, Cihan, Payen, Didier, Ince, Can
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3155675/
https://www.ncbi.nlm.nih.gov/pubmed/21695476
http://dx.doi.org/10.1007/s00134-011-2267-4
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author Legrand, Matthieu
Bezemer, Rick
Kandil, Asli
Demirci, Cihan
Payen, Didier
Ince, Can
author_facet Legrand, Matthieu
Bezemer, Rick
Kandil, Asli
Demirci, Cihan
Payen, Didier
Ince, Can
author_sort Legrand, Matthieu
collection PubMed
description PURPOSE: To study the role of renal hypoperfusion in development of renal microcirculatory dysfunction in endotoxemic rats. METHODS: Rats were randomized into four groups: a sham group (n = 6), a lipopolysaccharide (LPS) group (n = 6), a group in which LPS administration was followed by immediate fluid resuscitation which prevented the drop of renal blood flow (EARLY group) (n = 6), and a group in which LPS administration was followed by delayed (i.e., a 2-h delay) fluid resuscitation (LATE group) (n = 6). Renal blood flow was measured using a transit-time ultrasound flow probe. Microvascular perfusion and oxygenation distributions in the renal cortex were assessed using laser speckle imaging and phosphorimetry, respectively. Interleukin (IL)-6, IL-10, and tumor necrosis factor (TNF)-α were measured as markers of systemic inflammation. Furthermore, renal tissue samples were stained for leukocyte infiltration and inducible nitric oxide synthase (iNOS) expression in the kidney. RESULTS: LPS infusion worsened both microvascular perfusion and oxygenation distributions. Fluid resuscitation improved perfusion histograms but not oxygenation histograms. Improvement of microvascular perfusion was more pronounced in the EARLY group compared with the LATE group. Serum cytokine levels decreased in the resuscitated groups, with no difference between the EARLY and LATE groups. However, iNOS expression and leukocyte infiltration in glomeruli were lower in the EARLY group compared with the LATE group. CONCLUSIONS: In our model, prevention of endotoxemia-induced systemic hypotension by immediate fluid resuscitation (EARLY group) did not prevent systemic inflammatory activation (IL-6, IL-10, TNF-α) but did reduce renal inflammation (iNOS expression and glomerular leukocyte infiltration). However, it could not prevent reduced renal microvascular oxygenation. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00134-011-2267-4) contains supplementary material, which is available to authorized users.
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spelling pubmed-31556752011-09-21 The role of renal hypoperfusion in development of renal microcirculatory dysfunction in endotoxemic rats Legrand, Matthieu Bezemer, Rick Kandil, Asli Demirci, Cihan Payen, Didier Ince, Can Intensive Care Med Experimental PURPOSE: To study the role of renal hypoperfusion in development of renal microcirculatory dysfunction in endotoxemic rats. METHODS: Rats were randomized into four groups: a sham group (n = 6), a lipopolysaccharide (LPS) group (n = 6), a group in which LPS administration was followed by immediate fluid resuscitation which prevented the drop of renal blood flow (EARLY group) (n = 6), and a group in which LPS administration was followed by delayed (i.e., a 2-h delay) fluid resuscitation (LATE group) (n = 6). Renal blood flow was measured using a transit-time ultrasound flow probe. Microvascular perfusion and oxygenation distributions in the renal cortex were assessed using laser speckle imaging and phosphorimetry, respectively. Interleukin (IL)-6, IL-10, and tumor necrosis factor (TNF)-α were measured as markers of systemic inflammation. Furthermore, renal tissue samples were stained for leukocyte infiltration and inducible nitric oxide synthase (iNOS) expression in the kidney. RESULTS: LPS infusion worsened both microvascular perfusion and oxygenation distributions. Fluid resuscitation improved perfusion histograms but not oxygenation histograms. Improvement of microvascular perfusion was more pronounced in the EARLY group compared with the LATE group. Serum cytokine levels decreased in the resuscitated groups, with no difference between the EARLY and LATE groups. However, iNOS expression and leukocyte infiltration in glomeruli were lower in the EARLY group compared with the LATE group. CONCLUSIONS: In our model, prevention of endotoxemia-induced systemic hypotension by immediate fluid resuscitation (EARLY group) did not prevent systemic inflammatory activation (IL-6, IL-10, TNF-α) but did reduce renal inflammation (iNOS expression and glomerular leukocyte infiltration). However, it could not prevent reduced renal microvascular oxygenation. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00134-011-2267-4) contains supplementary material, which is available to authorized users. Springer-Verlag 2011-06-22 2011 /pmc/articles/PMC3155675/ /pubmed/21695476 http://dx.doi.org/10.1007/s00134-011-2267-4 Text en © The Author(s) 2011 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Experimental
Legrand, Matthieu
Bezemer, Rick
Kandil, Asli
Demirci, Cihan
Payen, Didier
Ince, Can
The role of renal hypoperfusion in development of renal microcirculatory dysfunction in endotoxemic rats
title The role of renal hypoperfusion in development of renal microcirculatory dysfunction in endotoxemic rats
title_full The role of renal hypoperfusion in development of renal microcirculatory dysfunction in endotoxemic rats
title_fullStr The role of renal hypoperfusion in development of renal microcirculatory dysfunction in endotoxemic rats
title_full_unstemmed The role of renal hypoperfusion in development of renal microcirculatory dysfunction in endotoxemic rats
title_short The role of renal hypoperfusion in development of renal microcirculatory dysfunction in endotoxemic rats
title_sort role of renal hypoperfusion in development of renal microcirculatory dysfunction in endotoxemic rats
topic Experimental
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3155675/
https://www.ncbi.nlm.nih.gov/pubmed/21695476
http://dx.doi.org/10.1007/s00134-011-2267-4
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