Biomarkers of exposure and effect—interpretation in human risk assessment

The effect of exposure to carcinogenic polycyclic aromatic hydrocarbons adsorbed onto respirable air particles (PM2.5, diameter < 2.5 μm) on DNA adducts and chromosomal aberrations was repeatedly studied in Prague, Czech Republic, in groups of policemen working in the downtown area and in bus dri...

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Autores principales: Sram, Radim J., Binkova, Blanka, Beskid, Olena, Milcova, Alena, Rossner, Pavel, Rossnerova, Andrea, Solansky, Ivo, Topinka, Jan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3156305/
https://www.ncbi.nlm.nih.gov/pubmed/21949552
http://dx.doi.org/10.1007/s11869-011-0133-8
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author Sram, Radim J.
Binkova, Blanka
Beskid, Olena
Milcova, Alena
Rossner, Pavel
Rossner, Pavel
Rossnerova, Andrea
Solansky, Ivo
Topinka, Jan
author_facet Sram, Radim J.
Binkova, Blanka
Beskid, Olena
Milcova, Alena
Rossner, Pavel
Rossner, Pavel
Rossnerova, Andrea
Solansky, Ivo
Topinka, Jan
author_sort Sram, Radim J.
collection PubMed
description The effect of exposure to carcinogenic polycyclic aromatic hydrocarbons adsorbed onto respirable air particles (PM2.5, diameter < 2.5 μm) on DNA adducts and chromosomal aberrations was repeatedly studied in Prague, Czech Republic, in groups of policemen working in the downtown area and in bus drivers. Personal exposure was evaluated using personal samplers during working shifts. DNA adducts were analyzed in lymphocytes by the (32)P-postlabeling assay and chromosomal aberrations were analyzed by conventional cytogenetic analysis and fluorescent in situ hybridization (FISH). The impact of environmental pollution on DNA adducts and chromosomal aberrations was studied in a total of 950 subjects. Our results suggest that the environmental exposure of nonsmokers to concentrations higher than 1 ng benzo[a]pyrene/m(3) represents a risk of DNA damage, as indicated by an increase in DNA adducts and the genomic frequency of translocations determined by FISH.
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spelling pubmed-31563052011-09-21 Biomarkers of exposure and effect—interpretation in human risk assessment Sram, Radim J. Binkova, Blanka Beskid, Olena Milcova, Alena Rossner, Pavel Rossner, Pavel Rossnerova, Andrea Solansky, Ivo Topinka, Jan Air Qual Atmos Health Article The effect of exposure to carcinogenic polycyclic aromatic hydrocarbons adsorbed onto respirable air particles (PM2.5, diameter < 2.5 μm) on DNA adducts and chromosomal aberrations was repeatedly studied in Prague, Czech Republic, in groups of policemen working in the downtown area and in bus drivers. Personal exposure was evaluated using personal samplers during working shifts. DNA adducts were analyzed in lymphocytes by the (32)P-postlabeling assay and chromosomal aberrations were analyzed by conventional cytogenetic analysis and fluorescent in situ hybridization (FISH). The impact of environmental pollution on DNA adducts and chromosomal aberrations was studied in a total of 950 subjects. Our results suggest that the environmental exposure of nonsmokers to concentrations higher than 1 ng benzo[a]pyrene/m(3) represents a risk of DNA damage, as indicated by an increase in DNA adducts and the genomic frequency of translocations determined by FISH. Springer Netherlands 2011-01-29 2011 /pmc/articles/PMC3156305/ /pubmed/21949552 http://dx.doi.org/10.1007/s11869-011-0133-8 Text en © The Author(s) 2011 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Article
Sram, Radim J.
Binkova, Blanka
Beskid, Olena
Milcova, Alena
Rossner, Pavel
Rossner, Pavel
Rossnerova, Andrea
Solansky, Ivo
Topinka, Jan
Biomarkers of exposure and effect—interpretation in human risk assessment
title Biomarkers of exposure and effect—interpretation in human risk assessment
title_full Biomarkers of exposure and effect—interpretation in human risk assessment
title_fullStr Biomarkers of exposure and effect—interpretation in human risk assessment
title_full_unstemmed Biomarkers of exposure and effect—interpretation in human risk assessment
title_short Biomarkers of exposure and effect—interpretation in human risk assessment
title_sort biomarkers of exposure and effect—interpretation in human risk assessment
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3156305/
https://www.ncbi.nlm.nih.gov/pubmed/21949552
http://dx.doi.org/10.1007/s11869-011-0133-8
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