Cargando…
Infectious alphavirus production from a simple plasmid transfection+
We have developed a new method for producing infectious double subgenomic alphaviruses from plasmids transfected into mammalian cells. A double subgenomic Sindbis virus (TE3'2J) was transcribed from a cytomegalovirus PolII promoter, which results in the production of infectious virus. Transfect...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3156776/ https://www.ncbi.nlm.nih.gov/pubmed/21771308 http://dx.doi.org/10.1186/1743-422X-8-356 |
_version_ | 1782210236312977408 |
---|---|
author | Steel, J Jordan Henderson, Brittney R Lama, Siddhi BC Olson, Ken E Geiss, Brian J |
author_facet | Steel, J Jordan Henderson, Brittney R Lama, Siddhi BC Olson, Ken E Geiss, Brian J |
author_sort | Steel, J Jordan |
collection | PubMed |
description | We have developed a new method for producing infectious double subgenomic alphaviruses from plasmids transfected into mammalian cells. A double subgenomic Sindbis virus (TE3'2J) was transcribed from a cytomegalovirus PolII promoter, which results in the production of infectious virus. Transfection of as little as 125 ng of plasmid is able to produce 1 × 10(8 )plaque forming units/ml (PFU/ml) of infectious virus 48 hours post-transfection. This system represents a more efficient method for producing recombinant Sindbis viruses. |
format | Online Article Text |
id | pubmed-3156776 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-31567762011-08-17 Infectious alphavirus production from a simple plasmid transfection+ Steel, J Jordan Henderson, Brittney R Lama, Siddhi BC Olson, Ken E Geiss, Brian J Virol J Methodology We have developed a new method for producing infectious double subgenomic alphaviruses from plasmids transfected into mammalian cells. A double subgenomic Sindbis virus (TE3'2J) was transcribed from a cytomegalovirus PolII promoter, which results in the production of infectious virus. Transfection of as little as 125 ng of plasmid is able to produce 1 × 10(8 )plaque forming units/ml (PFU/ml) of infectious virus 48 hours post-transfection. This system represents a more efficient method for producing recombinant Sindbis viruses. BioMed Central 2011-07-19 /pmc/articles/PMC3156776/ /pubmed/21771308 http://dx.doi.org/10.1186/1743-422X-8-356 Text en Copyright ©2011 Steel et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Methodology Steel, J Jordan Henderson, Brittney R Lama, Siddhi BC Olson, Ken E Geiss, Brian J Infectious alphavirus production from a simple plasmid transfection+ |
title | Infectious alphavirus production from a simple plasmid transfection+ |
title_full | Infectious alphavirus production from a simple plasmid transfection+ |
title_fullStr | Infectious alphavirus production from a simple plasmid transfection+ |
title_full_unstemmed | Infectious alphavirus production from a simple plasmid transfection+ |
title_short | Infectious alphavirus production from a simple plasmid transfection+ |
title_sort | infectious alphavirus production from a simple plasmid transfection+ |
topic | Methodology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3156776/ https://www.ncbi.nlm.nih.gov/pubmed/21771308 http://dx.doi.org/10.1186/1743-422X-8-356 |
work_keys_str_mv | AT steeljjordan infectiousalphavirusproductionfromasimpleplasmidtransfection AT hendersonbrittneyr infectiousalphavirusproductionfromasimpleplasmidtransfection AT lamasiddhibc infectiousalphavirusproductionfromasimpleplasmidtransfection AT olsonkene infectiousalphavirusproductionfromasimpleplasmidtransfection AT geissbrianj infectiousalphavirusproductionfromasimpleplasmidtransfection |