Infectious alphavirus production from a simple plasmid transfection+

We have developed a new method for producing infectious double subgenomic alphaviruses from plasmids transfected into mammalian cells. A double subgenomic Sindbis virus (TE3'2J) was transcribed from a cytomegalovirus PolII promoter, which results in the production of infectious virus. Transfect...

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Detalles Bibliográficos
Autores principales: Steel, J Jordan, Henderson, Brittney R, Lama, Siddhi BC, Olson, Ken E, Geiss, Brian J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3156776/
https://www.ncbi.nlm.nih.gov/pubmed/21771308
http://dx.doi.org/10.1186/1743-422X-8-356
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author Steel, J Jordan
Henderson, Brittney R
Lama, Siddhi BC
Olson, Ken E
Geiss, Brian J
author_facet Steel, J Jordan
Henderson, Brittney R
Lama, Siddhi BC
Olson, Ken E
Geiss, Brian J
author_sort Steel, J Jordan
collection PubMed
description We have developed a new method for producing infectious double subgenomic alphaviruses from plasmids transfected into mammalian cells. A double subgenomic Sindbis virus (TE3'2J) was transcribed from a cytomegalovirus PolII promoter, which results in the production of infectious virus. Transfection of as little as 125 ng of plasmid is able to produce 1 × 10(8 )plaque forming units/ml (PFU/ml) of infectious virus 48 hours post-transfection. This system represents a more efficient method for producing recombinant Sindbis viruses.
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spelling pubmed-31567762011-08-17 Infectious alphavirus production from a simple plasmid transfection+ Steel, J Jordan Henderson, Brittney R Lama, Siddhi BC Olson, Ken E Geiss, Brian J Virol J Methodology We have developed a new method for producing infectious double subgenomic alphaviruses from plasmids transfected into mammalian cells. A double subgenomic Sindbis virus (TE3'2J) was transcribed from a cytomegalovirus PolII promoter, which results in the production of infectious virus. Transfection of as little as 125 ng of plasmid is able to produce 1 × 10(8 )plaque forming units/ml (PFU/ml) of infectious virus 48 hours post-transfection. This system represents a more efficient method for producing recombinant Sindbis viruses. BioMed Central 2011-07-19 /pmc/articles/PMC3156776/ /pubmed/21771308 http://dx.doi.org/10.1186/1743-422X-8-356 Text en Copyright ©2011 Steel et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methodology
Steel, J Jordan
Henderson, Brittney R
Lama, Siddhi BC
Olson, Ken E
Geiss, Brian J
Infectious alphavirus production from a simple plasmid transfection+
title Infectious alphavirus production from a simple plasmid transfection+
title_full Infectious alphavirus production from a simple plasmid transfection+
title_fullStr Infectious alphavirus production from a simple plasmid transfection+
title_full_unstemmed Infectious alphavirus production from a simple plasmid transfection+
title_short Infectious alphavirus production from a simple plasmid transfection+
title_sort infectious alphavirus production from a simple plasmid transfection+
topic Methodology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3156776/
https://www.ncbi.nlm.nih.gov/pubmed/21771308
http://dx.doi.org/10.1186/1743-422X-8-356
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