Presence of Caffeic Acid in Flaxseed Lignan Macromolecule

Phenolic compounds were extracted from defatted flaxseeds using ethanol-dioxane (1:1, v/v). The crude extract obtained was purified using Amberlite XAD-16 column chromatography with water and methanol as mobile phases. RP-HPLC and SE-HPLC showed a lignan macromolecule (LM) as a dominant phenolic com...

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Autores principales: Kosińska, Agnieszka, Penkacik, Kamila, Wiczkowski, Wiesław, Amarowicz, Ryszard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2011
Materias:
Original Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3156902/
https://www.ncbi.nlm.nih.gov/pubmed/21766213
http://dx.doi.org/10.1007/s11130-011-0245-1
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author Kosińska, Agnieszka
Penkacik, Kamila
Wiczkowski, Wiesław
Amarowicz, Ryszard
author_facet Kosińska, Agnieszka
Penkacik, Kamila
Wiczkowski, Wiesław
Amarowicz, Ryszard
author_sort Kosińska, Agnieszka
collection PubMed
description Phenolic compounds were extracted from defatted flaxseeds using ethanol-dioxane (1:1, v/v). The crude extract obtained was purified using Amberlite XAD-16 column chromatography with water and methanol as mobile phases. RP-HPLC and SE-HPLC showed a lignan macromolecule (LM) as a dominant phenolic compound in the purified extract. After the alkaline hydrolysis of LM caffeic acid glucoside (CaAG) was isolated using a semi-preparative HPLC and its structure was confirmed by LC-ESI-MS. In LM of the investigated flaxseed, one molecule of caffeic acid corresponded with five molecules of p-coumaric acid and two molecules of ferulic acid. The presence of caffeic acid in the lignan molecule might be very beneficial due to its high antioxidant activity.
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spelling pubmed-31569022011-09-21 Presence of Caffeic Acid in Flaxseed Lignan Macromolecule Kosińska, Agnieszka Penkacik, Kamila Wiczkowski, Wiesław Amarowicz, Ryszard Plant Foods Hum Nutr Original Paper Phenolic compounds were extracted from defatted flaxseeds using ethanol-dioxane (1:1, v/v). The crude extract obtained was purified using Amberlite XAD-16 column chromatography with water and methanol as mobile phases. RP-HPLC and SE-HPLC showed a lignan macromolecule (LM) as a dominant phenolic compound in the purified extract. After the alkaline hydrolysis of LM caffeic acid glucoside (CaAG) was isolated using a semi-preparative HPLC and its structure was confirmed by LC-ESI-MS. In LM of the investigated flaxseed, one molecule of caffeic acid corresponded with five molecules of p-coumaric acid and two molecules of ferulic acid. The presence of caffeic acid in the lignan molecule might be very beneficial due to its high antioxidant activity. Springer US 2011-07-16 2011 /pmc/articles/PMC3156902/ /pubmed/21766213 http://dx.doi.org/10.1007/s11130-011-0245-1 Text en © The Author(s) 2011 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Original Paper
Kosińska, Agnieszka
Penkacik, Kamila
Wiczkowski, Wiesław
Amarowicz, Ryszard
Presence of Caffeic Acid in Flaxseed Lignan Macromolecule
title Presence of Caffeic Acid in Flaxseed Lignan Macromolecule
title_full Presence of Caffeic Acid in Flaxseed Lignan Macromolecule
title_fullStr Presence of Caffeic Acid in Flaxseed Lignan Macromolecule
title_full_unstemmed Presence of Caffeic Acid in Flaxseed Lignan Macromolecule
title_short Presence of Caffeic Acid in Flaxseed Lignan Macromolecule
title_sort presence of caffeic acid in flaxseed lignan macromolecule
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3156902/
https://www.ncbi.nlm.nih.gov/pubmed/21766213
http://dx.doi.org/10.1007/s11130-011-0245-1
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AT wiczkowskiwiesław presenceofcaffeicacidinflaxseedlignanmacromolecule
AT amarowiczryszard presenceofcaffeicacidinflaxseedlignanmacromolecule

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