Cargando…

Development of one-step SYBR Green real-time RT-PCR for quantifying bovine viral diarrhea virus type-1 and its comparison with conventional RT-PCR

BACKGROUND: Bovine viral diarrhea virus (BVDV) is a worldwide pathogen in cattle and acts as a surrogate model for hepatitis C virus (HCV). One-step real-time fluorogenic quantitative reverse transcription polymerase chain reaction (RT-PCR) assay based on SYBR Green I dye has not been established fo...

Descripción completa

Detalles Bibliográficos
Autores principales:	Zhang, Ni, Liu, Zhengwen, Han, Qunying, Qiu, Jianming, Chen, Jinghong, Zhang, Guoyu, Li, Zhu, Lou, Sai, Li, Na
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2011
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3157457/ https://www.ncbi.nlm.nih.gov/pubmed/21798067 http://dx.doi.org/10.1186/1743-422X-8-374

Ejemplares similares

A SYBR Green RT-PCR assay in single tube to detect human and bovine noroviruses and control for inhibition
por: Scipioni, Alexandra, et al.
Publicado: (2008)

Lower cost alternatives for molecular diagnosis of COVID-19: conventional RT-PCR and SYBR Green-based RT-qPCR
por: Dorlass, Erick Gustavo, et al.
Publicado: (2020)

Development and evaluation of a SYBR Green real-time RT-PCR assay for detection of avian hepatitis E virus
por: Zhao, Qin, et al.
Publicado: (2015)

Detection of bovine viral diarrhea virus genotype 1 in aerosol by a real time RT-PCR assay
por: Hou, Peili, et al.
Publicado: (2020)

Selection of internal reference genes for SYBR green qRT-PCR studies of rhesus monkey (Macaca mulatta) tissues
por: Ahn, Kung, et al.
Publicado: (2008)

Comparison of SYBR green I real-time RT-PCR with conventional agarose gel-based RT-PCR for the diagnosis of infectious bronchitis virus infection in chickens in Morocco
por: Fellahi, Siham, et al.
Publicado: (2016)

Development of a Conventional RT-PCR Assay for Rapid Detection of Porcine Deltacoronavirus with the Same Detection Limit as a SYBR Green-Based Real-Time RT-PCR Assay
por: Ma, Lei, et al.
Publicado: (2018)

Quantification of Hantaan Virus with a SYBR Green Ⅰ-Based One-Step qRT-PCR Assay
por: Jiang, Wei, et al.
Publicado: (2013)

Development of universal SYBR Green real-time RT-PCR for the rapid detection and quantitation of bovine and porcine toroviruses
por: Hosmillo, Myra D.T., et al.
Publicado: (2010)

One-step multiplex real time RT-PCR for the detection of bovine respiratory syncytial virus, bovine herpesvirus 1 and bovine parainfluenza virus 3
por: Thonur, Leenadevi, et al.
Publicado: (2012)

Design and evaluation of a unique SYBR Green real-time RT-PCR assay for quantification of five major cytokines in cattle, sheep and goats
por: Puech, Carinne, et al.
Publicado: (2015)

A one step real-time RT-PCR assay for the quantitation of Wheat yellow mosaic virus (WYMV)
por: Liu, Wenwen, et al.
Publicado: (2013)

Correction: Quantification of Hantaan Virus with a SYBR Green Ⅰ-Based One-Step qRT-PCR Assay
por: Jiang, Wei, et al.
Publicado: (2013)

Development of SYBR Green real-time RT-PCR for rapid detection, quantitation and diagnosis of unclassified bovine enteric calicivirus
por: Park, Sang-Ik, et al.
Publicado: (2009)

Development of a SYBR Green I based real-time RT-PCR assay for detection and quantification of bovine coronavirus
por: Amer, Haitham M., et al.
Publicado: (2011)

Performance of Simplexa Dengue Molecular Assay Compared to Conventional and SYBR Green RT-PCR for Detection of Dengue Infection in Indonesia
por: Sasmono, R. Tedjo, et al.
Publicado: (2014)

Development and evaluation of one step single tube multiplex RT-PCR for rapid detection and typing of dengue viruses
por: Saxena, Parag, et al.
Publicado: (2008)

Detection of 22 common leukemic fusion genes using a single-step multiplex qRT-PCR-based assay
por: Lyu, Xiaodong, et al.
Publicado: (2017)

Development of a real-time RT-PCR assay for the detection of pan-human parechoviruses
por: Lu, Huanhuan, et al.
Publicado: (2021)

The development of a rapid SYBR one step real-time RT-PCR for detection of porcine reproductive and respiratory syndrome virus
por: Tian, Hong, et al.
Publicado: (2010)

Selection of optimal reference genes for normalization in quantitative RT-PCR
por: Chervoneva, Inna, et al.
Publicado: (2010)

SYBR Green based real-time RT-PCR assay for detection and genotype prediction of bovine noroviruses and assessment of clinical significance in Norway
por: Jor, Evert, et al.
Publicado: (2010)

Fluorogenic RT–PCR assay (TaqMan) for detection and classification of bovine viral diarrhea virus
por: Bhudevi, Bodreddigari, et al.
Publicado: (2001)

Development of a SYBR green-based real-time RT-PCR assay for rapid detection of the emerging swine acute diarrhea syndrome coronavirus
por: Ma, Lei, et al.
Publicado: (2019)

Combination of conventional immunohistochemistry and qRT-PCR to detect ALK rearrangement
por: Shan, Ling, et al.
Publicado: (2014)

Real-time RT-PCR high-resolution melting curve analysis and multiplex RT-PCR to detect and differentiate grapevine leafroll-associated virus 3 variant groups I, II, III and VI
por: Bester, Rachelle, et al.
Publicado: (2012)

A Broadly Reactive One-Step SYBR Green I Real-Time RT-PCR Assay for Rapid Detection of Murine Norovirus
por: Hanaki, Ken-Ichi, et al.
Publicado: (2014)

Quantifying 35 transcripts in a single tube: model-based calibration of the GeXP multiplex RT-PCR assay
por: Marquardt, Pauline, et al.
Publicado: (2021)

Protocol: a highly sensitive RT-PCR method for detection and quantification of microRNAs
por: Varkonyi-Gasic, Erika, et al.
Publicado: (2007)

Preamplification techniques for real-time RT-PCR analyses of endomyocardial biopsies
por: Noutsias, Michel, et al.
Publicado: (2008)

Specific and sensitive quantitative RT-PCR of miRNAs with DNA primers
por: Balcells, Ingrid, et al.
Publicado: (2011)

Data-driven normalization strategies for high-throughput quantitative RT-PCR
por: Mar, Jessica C, et al.
Publicado: (2009)

Applicability of RNA standards for evaluating RT-qPCR assays and platforms
por: Devonshire, Alison S, et al.
Publicado: (2011)

Comparison of real-time SYBR green dengue assay with real-time taqman RT-PCR dengue assay and the conventional nested PCR for diagnosis of primary and secondary dengue infection
por: Paudel, Damodar, et al.
Publicado: (2011)

Development of a reverse transcription droplet digital PCR (RT-ddPCR) assay for sensitive detection of simian immunodeficiency virus (SIV)
por: Long, Samuel, et al.
Publicado: (2021)

Real-time RT-PCR assay to detect Granada virus and the related Massilia and Arrabida phleboviruses
por: Davó, Laura, et al.
Publicado: (2020)

Diagnosis of Barmah Forest Virus Infection by a Nested Real-Time SYBR Green RT-PCR Assay
por: Hueston, Linda, et al.
Publicado: (2013)

Development and application of a SYBR green RT-PCR for first line screening and quantification of porcine sapovirus infection
por: Mauroy, Axel, et al.
Publicado: (2012)

Identification of reference genes in blood before and after entering the plateau for SYBR green RT-qPCR studies
por: Xiao, Jun, et al.
Publicado: (2017)

Development of a One-Step SYBR Green I Real-Time RT-PCR Assay for the Detection and Quantitation of Araraquara and Rio Mamore Hantavirus
por: Machado, Alex Martins, et al.
Publicado: (2013)

Cannot write session to /tmp/vufind_sessions/sess_9nld4m8jmm9dh248bipo14je5r