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Identification of two linear B-cell epitopes from West Nile virus NS1 by screening a phage-displayed random peptide library

BACKGROUND: The West Nile virus (WNV) nonstructural protein 1 (NS1) is an important antigenic protein that elicits protective antibody responses in animals and can be used for the serological diagnosis of WNV infection. Although previous work has demonstrated the vital role of WNV NS1-specific antib...

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Autores principales: Sun, En-Cheng, Ma, Jian-Nan, Liu, Ni-Hong, Yang, Tao, Zhao, Jing, Geng, Hong-Wei, Wang, Ling-Feng, Qin, Yong-Li, Bu, Zhi-Gao, Yang, Yin-Hui, Lunt, Ross A, Wang, Lin-Fa, Wu, Dong-Lai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3158561/
https://www.ncbi.nlm.nih.gov/pubmed/21729328
http://dx.doi.org/10.1186/1471-2180-11-160
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author Sun, En-Cheng
Ma, Jian-Nan
Liu, Ni-Hong
Yang, Tao
Zhao, Jing
Geng, Hong-Wei
Wang, Ling-Feng
Qin, Yong-Li
Bu, Zhi-Gao
Yang, Yin-Hui
Lunt, Ross A
Wang, Lin-Fa
Wu, Dong-Lai
author_facet Sun, En-Cheng
Ma, Jian-Nan
Liu, Ni-Hong
Yang, Tao
Zhao, Jing
Geng, Hong-Wei
Wang, Ling-Feng
Qin, Yong-Li
Bu, Zhi-Gao
Yang, Yin-Hui
Lunt, Ross A
Wang, Lin-Fa
Wu, Dong-Lai
author_sort Sun, En-Cheng
collection PubMed
description BACKGROUND: The West Nile virus (WNV) nonstructural protein 1 (NS1) is an important antigenic protein that elicits protective antibody responses in animals and can be used for the serological diagnosis of WNV infection. Although previous work has demonstrated the vital role of WNV NS1-specific antibody responses, the specific epitopes in the NS1 have not been identified. RESULTS: The present study describes the identification of two linear B-cell epitopes in WNV NS1 through screening a phage-displayed random 12-mer peptide library with two monoclonal antibodies (mAbs) 3C7 and 4D1 that directed against the NS1. The mAbs 3C7 and 4D1 recognized phages displaying peptides with the consensus motifs LTATTEK and VVDGPETKEC, respectively. Exact sequences of both motifs were found in the NS1 ((895)LTATTEK(901 )and (925)VVDGPETKEC(934)). Further identification of the displayed B cell epitopes were conducted using a set of truncated peptides expressed as MBP fusion proteins. The data indicated that (896)TATTEK(901 )and(925)VVDGPETKEC(934 )are minimal determinants of the linear B cell epitopes recognized by the mAbs 3C7 and 4D1, respectively. Antibodies present in the serum of WNV-positive horses recognized the minimal linear epitopes in Western blot analysis, indicating that the two peptides are antigenic in horses during infection. Furthermore, we found that the epitope recognized by 3C7 is conserved only among WNV strains, whereas the epitope recognized by 4D1 is a common motif shared among WNV and other members of Japanese encephalitis virus (JEV) serocomplex. CONCLUSIONS: We identified TATTEK and VVDGPETKEC as NS1-specific linear B-cell epitopes recognized by the mAbs 3C7 and 4D1, respectively. The knowledge and reagents generated in this study may have potential applications in differential diagnosis and the development of epitope-based marker vaccines against WNV and other viruses of JEV serocomplex.
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spelling pubmed-31585612011-08-20 Identification of two linear B-cell epitopes from West Nile virus NS1 by screening a phage-displayed random peptide library Sun, En-Cheng Ma, Jian-Nan Liu, Ni-Hong Yang, Tao Zhao, Jing Geng, Hong-Wei Wang, Ling-Feng Qin, Yong-Li Bu, Zhi-Gao Yang, Yin-Hui Lunt, Ross A Wang, Lin-Fa Wu, Dong-Lai BMC Microbiol Research Article BACKGROUND: The West Nile virus (WNV) nonstructural protein 1 (NS1) is an important antigenic protein that elicits protective antibody responses in animals and can be used for the serological diagnosis of WNV infection. Although previous work has demonstrated the vital role of WNV NS1-specific antibody responses, the specific epitopes in the NS1 have not been identified. RESULTS: The present study describes the identification of two linear B-cell epitopes in WNV NS1 through screening a phage-displayed random 12-mer peptide library with two monoclonal antibodies (mAbs) 3C7 and 4D1 that directed against the NS1. The mAbs 3C7 and 4D1 recognized phages displaying peptides with the consensus motifs LTATTEK and VVDGPETKEC, respectively. Exact sequences of both motifs were found in the NS1 ((895)LTATTEK(901 )and (925)VVDGPETKEC(934)). Further identification of the displayed B cell epitopes were conducted using a set of truncated peptides expressed as MBP fusion proteins. The data indicated that (896)TATTEK(901 )and(925)VVDGPETKEC(934 )are minimal determinants of the linear B cell epitopes recognized by the mAbs 3C7 and 4D1, respectively. Antibodies present in the serum of WNV-positive horses recognized the minimal linear epitopes in Western blot analysis, indicating that the two peptides are antigenic in horses during infection. Furthermore, we found that the epitope recognized by 3C7 is conserved only among WNV strains, whereas the epitope recognized by 4D1 is a common motif shared among WNV and other members of Japanese encephalitis virus (JEV) serocomplex. CONCLUSIONS: We identified TATTEK and VVDGPETKEC as NS1-specific linear B-cell epitopes recognized by the mAbs 3C7 and 4D1, respectively. The knowledge and reagents generated in this study may have potential applications in differential diagnosis and the development of epitope-based marker vaccines against WNV and other viruses of JEV serocomplex. BioMed Central 2011-07-06 /pmc/articles/PMC3158561/ /pubmed/21729328 http://dx.doi.org/10.1186/1471-2180-11-160 Text en Copyright ©2011 Sun et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Sun, En-Cheng
Ma, Jian-Nan
Liu, Ni-Hong
Yang, Tao
Zhao, Jing
Geng, Hong-Wei
Wang, Ling-Feng
Qin, Yong-Li
Bu, Zhi-Gao
Yang, Yin-Hui
Lunt, Ross A
Wang, Lin-Fa
Wu, Dong-Lai
Identification of two linear B-cell epitopes from West Nile virus NS1 by screening a phage-displayed random peptide library
title Identification of two linear B-cell epitopes from West Nile virus NS1 by screening a phage-displayed random peptide library
title_full Identification of two linear B-cell epitopes from West Nile virus NS1 by screening a phage-displayed random peptide library
title_fullStr Identification of two linear B-cell epitopes from West Nile virus NS1 by screening a phage-displayed random peptide library
title_full_unstemmed Identification of two linear B-cell epitopes from West Nile virus NS1 by screening a phage-displayed random peptide library
title_short Identification of two linear B-cell epitopes from West Nile virus NS1 by screening a phage-displayed random peptide library
title_sort identification of two linear b-cell epitopes from west nile virus ns1 by screening a phage-displayed random peptide library
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3158561/
https://www.ncbi.nlm.nih.gov/pubmed/21729328
http://dx.doi.org/10.1186/1471-2180-11-160
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