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Identification and characterization of alkaline serine protease from goat skin surface metagenome

Metagenomic DNA isolated from goat skin surface was used to construct plasmid DNA library in Escherichia coli DH10B. Recombinant clones were screened for functional protease activity on skim milk agar plates. Upon screening 70,000 clones, a clone carrying recombinant plasmid pSP1 exhibited protease...

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Detalles Bibliográficos
Autores principales:	Pushpam, Paul Lavanya, Rajesh, Thangamani, Gunasekaran, Paramasamy
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Springer 2011
Materias:	Original
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3159910/ https://www.ncbi.nlm.nih.gov/pubmed/21906326 http://dx.doi.org/10.1186/2191-0855-1-3

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