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Economics of membrane occupancy and respiro-fermentation
The simultaneous utilization of efficient respiration and inefficient fermentation even in the presence of abundant oxygen is a puzzling phenomenon commonly observed in bacteria, yeasts, and cancer cells. Despite extensive research, the biochemical basis for this phenomenon remains obscure. We hypot...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
European Molecular Biology Organization
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3159977/ https://www.ncbi.nlm.nih.gov/pubmed/21694717 http://dx.doi.org/10.1038/msb.2011.34 |
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author | Zhuang, Kai Vemuri, Goutham N Mahadevan, Radhakrishnan |
author_facet | Zhuang, Kai Vemuri, Goutham N Mahadevan, Radhakrishnan |
author_sort | Zhuang, Kai |
collection | PubMed |
description | The simultaneous utilization of efficient respiration and inefficient fermentation even in the presence of abundant oxygen is a puzzling phenomenon commonly observed in bacteria, yeasts, and cancer cells. Despite extensive research, the biochemical basis for this phenomenon remains obscure. We hypothesize that the outcome of a competition for membrane space between glucose transporters and respiratory chain (which we refer to as economics of membrane occupancy) proteins influences respiration and fermentation. By incorporating a sole constraint based on this concept in the genome-scale metabolic model of Escherichia coli, we were able to simulate respiro-fermentation. Further analysis of the impact of this constraint revealed differential utilization of the cytochromes and faster glucose uptake under anaerobic conditions than under aerobic conditions. Based on these simulations, we propose that bacterial cells manage the composition of their cytoplasmic membrane to maintain optimal ATP production by switching between oxidative and substrate-level phosphorylation. These results suggest that the membrane occupancy constraint may be a fundamental governing constraint of cellular metabolism and physiology, and establishes a direct link between cell morphology and physiology. |
format | Online Article Text |
id | pubmed-3159977 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | European Molecular Biology Organization |
record_format | MEDLINE/PubMed |
spelling | pubmed-31599772011-08-24 Economics of membrane occupancy and respiro-fermentation Zhuang, Kai Vemuri, Goutham N Mahadevan, Radhakrishnan Mol Syst Biol Article The simultaneous utilization of efficient respiration and inefficient fermentation even in the presence of abundant oxygen is a puzzling phenomenon commonly observed in bacteria, yeasts, and cancer cells. Despite extensive research, the biochemical basis for this phenomenon remains obscure. We hypothesize that the outcome of a competition for membrane space between glucose transporters and respiratory chain (which we refer to as economics of membrane occupancy) proteins influences respiration and fermentation. By incorporating a sole constraint based on this concept in the genome-scale metabolic model of Escherichia coli, we were able to simulate respiro-fermentation. Further analysis of the impact of this constraint revealed differential utilization of the cytochromes and faster glucose uptake under anaerobic conditions than under aerobic conditions. Based on these simulations, we propose that bacterial cells manage the composition of their cytoplasmic membrane to maintain optimal ATP production by switching between oxidative and substrate-level phosphorylation. These results suggest that the membrane occupancy constraint may be a fundamental governing constraint of cellular metabolism and physiology, and establishes a direct link between cell morphology and physiology. European Molecular Biology Organization 2011-06-21 /pmc/articles/PMC3159977/ /pubmed/21694717 http://dx.doi.org/10.1038/msb.2011.34 Text en Copyright © 2011, EMBO and Macmillan Publishers Limited https://creativecommons.org/licenses/by-nc-nd/3.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Noncommercial No Derivative Works 3.0 Unported License, which permits distribution and reproduction in any medium, provided the original author and source are credited. This license does not permit commercial exploitation or the creation of derivative works without specific permission. |
spellingShingle | Article Zhuang, Kai Vemuri, Goutham N Mahadevan, Radhakrishnan Economics of membrane occupancy and respiro-fermentation |
title | Economics of membrane occupancy and respiro-fermentation |
title_full | Economics of membrane occupancy and respiro-fermentation |
title_fullStr | Economics of membrane occupancy and respiro-fermentation |
title_full_unstemmed | Economics of membrane occupancy and respiro-fermentation |
title_short | Economics of membrane occupancy and respiro-fermentation |
title_sort | economics of membrane occupancy and respiro-fermentation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3159977/ https://www.ncbi.nlm.nih.gov/pubmed/21694717 http://dx.doi.org/10.1038/msb.2011.34 |
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