Cargando…
Increased CD4+/CD8+ Double-Positive T Cells in Chronic Chagasic Patients
BACKGROUND: CD4+/CD8+ double positive (DP) T cells have been described in healthy individuals as well as in patients with autoimmune and chronic infectious diseases. In chronic viral infections, this cell subset has effector memory phenotype and displays antigen specificity. No previous studies of d...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3160296/ https://www.ncbi.nlm.nih.gov/pubmed/21886854 http://dx.doi.org/10.1371/journal.pntd.0001294 |
_version_ | 1782210528876167168 |
---|---|
author | Giraldo, Nicolas A. Bolaños, Natalia I. Cuellar, Adriana Guzman, Fanny Uribe, Ana Maria Bedoya, Astrid Olaya, Natalia Cucunubá, Zulma M. Roa, Nubia Rosas, Fernando Velasco, Víctor Puerta, Concepción J. González, John M. |
author_facet | Giraldo, Nicolas A. Bolaños, Natalia I. Cuellar, Adriana Guzman, Fanny Uribe, Ana Maria Bedoya, Astrid Olaya, Natalia Cucunubá, Zulma M. Roa, Nubia Rosas, Fernando Velasco, Víctor Puerta, Concepción J. González, John M. |
author_sort | Giraldo, Nicolas A. |
collection | PubMed |
description | BACKGROUND: CD4+/CD8+ double positive (DP) T cells have been described in healthy individuals as well as in patients with autoimmune and chronic infectious diseases. In chronic viral infections, this cell subset has effector memory phenotype and displays antigen specificity. No previous studies of double positive T cells in parasite infections have been carried out. METHODOLOGY/PRINCIPAL FINDINGS: Seventeen chronic chagasic patients (7 asymptomatic and 10 symptomatic) and 24 non-infected donors, including 12 healthy and 12 with non-chagasic cardiomyopathy donors were analyzed. Peripheral blood was stained for CD3, CD4, CD8, HLA-DR and CD38, and lymphocytes for intracellular perforin. Antigen specificity was assessed using HLA*A2 tetramers loaded with T. cruzi K1 or influenza virus epitopes. Surface expression of CD107 and intracellular IFN-γ production were determined in K1-specific DP T cells from 11 chagasic donors. Heart tissue from a chronic chagasic patient was stained for both CD8 and CD4 by immunochemistry. Chagasic patients showed higher frequencies of DP T cells (2.1%±0.9) compared with healthy (1.1%±0.5) and non-chagasic cardiomyopathy (1.2%±0.4) donors. DP T cells from Chagasic patients also expressed more HLA-DR, CD38 and perforin and had higher frequencies of T. cruzi K1-specific cells. IFN-γ production in K1-specific cells was higher in asymptomatic patients after polyclonal stimulation, while these cells tended to degranulate more in symptomatic donors. Immunochemistry revealed that double positive T cells infiltrate the cardiac tissue of a chagasic donor. CONCLUSIONS: Chagasic patients have higher percentages of circulating double positive T cells expressing activation markers, potential effector molecules and greater class I antigenic specificity against T. cruzi. Although K1 tetramer positive DP T cell produced little IFN-γ, they displayed degranulation activity that was increased in symptomatic patients. Moreover, K1-specific DP T cells can migrate to the heart tissue. |
format | Online Article Text |
id | pubmed-3160296 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-31602962011-08-30 Increased CD4+/CD8+ Double-Positive T Cells in Chronic Chagasic Patients Giraldo, Nicolas A. Bolaños, Natalia I. Cuellar, Adriana Guzman, Fanny Uribe, Ana Maria Bedoya, Astrid Olaya, Natalia Cucunubá, Zulma M. Roa, Nubia Rosas, Fernando Velasco, Víctor Puerta, Concepción J. González, John M. PLoS Negl Trop Dis Research Article BACKGROUND: CD4+/CD8+ double positive (DP) T cells have been described in healthy individuals as well as in patients with autoimmune and chronic infectious diseases. In chronic viral infections, this cell subset has effector memory phenotype and displays antigen specificity. No previous studies of double positive T cells in parasite infections have been carried out. METHODOLOGY/PRINCIPAL FINDINGS: Seventeen chronic chagasic patients (7 asymptomatic and 10 symptomatic) and 24 non-infected donors, including 12 healthy and 12 with non-chagasic cardiomyopathy donors were analyzed. Peripheral blood was stained for CD3, CD4, CD8, HLA-DR and CD38, and lymphocytes for intracellular perforin. Antigen specificity was assessed using HLA*A2 tetramers loaded with T. cruzi K1 or influenza virus epitopes. Surface expression of CD107 and intracellular IFN-γ production were determined in K1-specific DP T cells from 11 chagasic donors. Heart tissue from a chronic chagasic patient was stained for both CD8 and CD4 by immunochemistry. Chagasic patients showed higher frequencies of DP T cells (2.1%±0.9) compared with healthy (1.1%±0.5) and non-chagasic cardiomyopathy (1.2%±0.4) donors. DP T cells from Chagasic patients also expressed more HLA-DR, CD38 and perforin and had higher frequencies of T. cruzi K1-specific cells. IFN-γ production in K1-specific cells was higher in asymptomatic patients after polyclonal stimulation, while these cells tended to degranulate more in symptomatic donors. Immunochemistry revealed that double positive T cells infiltrate the cardiac tissue of a chagasic donor. CONCLUSIONS: Chagasic patients have higher percentages of circulating double positive T cells expressing activation markers, potential effector molecules and greater class I antigenic specificity against T. cruzi. Although K1 tetramer positive DP T cell produced little IFN-γ, they displayed degranulation activity that was increased in symptomatic patients. Moreover, K1-specific DP T cells can migrate to the heart tissue. Public Library of Science 2011-08-23 /pmc/articles/PMC3160296/ /pubmed/21886854 http://dx.doi.org/10.1371/journal.pntd.0001294 Text en Giraldo et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Giraldo, Nicolas A. Bolaños, Natalia I. Cuellar, Adriana Guzman, Fanny Uribe, Ana Maria Bedoya, Astrid Olaya, Natalia Cucunubá, Zulma M. Roa, Nubia Rosas, Fernando Velasco, Víctor Puerta, Concepción J. González, John M. Increased CD4+/CD8+ Double-Positive T Cells in Chronic Chagasic Patients |
title | Increased CD4+/CD8+ Double-Positive T Cells in Chronic Chagasic Patients |
title_full | Increased CD4+/CD8+ Double-Positive T Cells in Chronic Chagasic Patients |
title_fullStr | Increased CD4+/CD8+ Double-Positive T Cells in Chronic Chagasic Patients |
title_full_unstemmed | Increased CD4+/CD8+ Double-Positive T Cells in Chronic Chagasic Patients |
title_short | Increased CD4+/CD8+ Double-Positive T Cells in Chronic Chagasic Patients |
title_sort | increased cd4+/cd8+ double-positive t cells in chronic chagasic patients |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3160296/ https://www.ncbi.nlm.nih.gov/pubmed/21886854 http://dx.doi.org/10.1371/journal.pntd.0001294 |
work_keys_str_mv | AT giraldonicolasa increasedcd4cd8doublepositivetcellsinchronicchagasicpatients AT bolanosnataliai increasedcd4cd8doublepositivetcellsinchronicchagasicpatients AT cuellaradriana increasedcd4cd8doublepositivetcellsinchronicchagasicpatients AT guzmanfanny increasedcd4cd8doublepositivetcellsinchronicchagasicpatients AT uribeanamaria increasedcd4cd8doublepositivetcellsinchronicchagasicpatients AT bedoyaastrid increasedcd4cd8doublepositivetcellsinchronicchagasicpatients AT olayanatalia increasedcd4cd8doublepositivetcellsinchronicchagasicpatients AT cucunubazulmam increasedcd4cd8doublepositivetcellsinchronicchagasicpatients AT roanubia increasedcd4cd8doublepositivetcellsinchronicchagasicpatients AT rosasfernando increasedcd4cd8doublepositivetcellsinchronicchagasicpatients AT velascovictor increasedcd4cd8doublepositivetcellsinchronicchagasicpatients AT puertaconcepcionj increasedcd4cd8doublepositivetcellsinchronicchagasicpatients AT gonzalezjohnm increasedcd4cd8doublepositivetcellsinchronicchagasicpatients |