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Endotoxin-free purification for the isolation of Bovine Viral Diarrhoea Virus E2 protein from insoluble inclusion body aggregates

BACKGROUND: Protein expression in Escherichia coli may result in the recombinant protein being expressed as insoluble inclusion bodies. In addition, proteins purified from E. coli contain endotoxins which need to be removed for in vivo applications. The structural protein, E2, from Bovine Viral Diar...

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Detalles Bibliográficos
Autores principales:	Cavallaro, Antonino S, Mahony, Donna, Commins, Margaret, Mahony, Timothy J, Mitter, Neena
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2011
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3160874/ https://www.ncbi.nlm.nih.gov/pubmed/21787435 http://dx.doi.org/10.1186/1475-2859-10-57

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