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Chromosomal painting and ZW sex chromosomes differentiation in Characidium (Characiformes, Crenuchidae)
BACKGROUND: The Characidium (a Neotropical fish group) have a conserved diploid number (2n = 50), but show remarkable differences among species and populations in relation to sex chromosome systems and location of nucleolus organizer regions (NOR). In this study, we isolated a W-specific probe for t...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3160954/ https://www.ncbi.nlm.nih.gov/pubmed/21787398 http://dx.doi.org/10.1186/1471-2156-12-65 |
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author | Machado, Tatiana C Pansonato-Alves, José C Pucci, Marcela B Nogaroto, Viviane Almeida, Mara C Oliveira, Claudio Foresti, Fausto Bertollo, Luiz AC Moreira-Filho, Orlando Artoni, Roberto F Vicari, Marcelo R |
author_facet | Machado, Tatiana C Pansonato-Alves, José C Pucci, Marcela B Nogaroto, Viviane Almeida, Mara C Oliveira, Claudio Foresti, Fausto Bertollo, Luiz AC Moreira-Filho, Orlando Artoni, Roberto F Vicari, Marcelo R |
author_sort | Machado, Tatiana C |
collection | PubMed |
description | BACKGROUND: The Characidium (a Neotropical fish group) have a conserved diploid number (2n = 50), but show remarkable differences among species and populations in relation to sex chromosome systems and location of nucleolus organizer regions (NOR). In this study, we isolated a W-specific probe for the Characidium and characterized six Characidium species/populations using cytogenetic procedures. We analyzed the origin and differentiation of sex and NOR-bearing chromosomes by chromosome painting in populations of Characidium to reveal their evolution, phylogeny, and biogeography. RESULTS: A W-specific probe for efficient chromosome painting was isolated by microdissection and degenerate oligonucleotide primed-polymerase chain reaction (DOP-PCR) amplification of W chromosomes from C. gomesi. The W probe generated weak signals dispersed on the proto sex chromosomes in C. zebra, dispersed signals in both W and Z chromosomes in C. lauroi and, in C. gomesi populations revealed a proximal site on the long arms of the Z chromosome and the entire W chromosome. All populations showed small terminal W probe sites in some autosomes. The 18S rDNA revealed distinctive patterns for each analyzed species/population with regard to proto sex chromosome, sex chromosome pair, and autosome location. CONCLUSIONS: The results from dual-color fluorescence in situ hybridization (dual-color FISH) using W and 18S rDNA probes allowed us to infer the putative evolutionary pathways for the differentiation of sex chromosomes and NORs, from structural rearrangements in a sex proto-chromosome, followed by gene erosion and heterochromatin amplification, morphological differentiation of the sex chromosomal pair, and NOR transposition, giving rise to the distinctive patterns observed among species/populations of Characidium. Biogeographic isolation and differentiation of sex chromosomes seem to have played a major role in the speciation process in this group of fish. |
format | Online Article Text |
id | pubmed-3160954 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-31609542011-08-25 Chromosomal painting and ZW sex chromosomes differentiation in Characidium (Characiformes, Crenuchidae) Machado, Tatiana C Pansonato-Alves, José C Pucci, Marcela B Nogaroto, Viviane Almeida, Mara C Oliveira, Claudio Foresti, Fausto Bertollo, Luiz AC Moreira-Filho, Orlando Artoni, Roberto F Vicari, Marcelo R BMC Genet Research Article BACKGROUND: The Characidium (a Neotropical fish group) have a conserved diploid number (2n = 50), but show remarkable differences among species and populations in relation to sex chromosome systems and location of nucleolus organizer regions (NOR). In this study, we isolated a W-specific probe for the Characidium and characterized six Characidium species/populations using cytogenetic procedures. We analyzed the origin and differentiation of sex and NOR-bearing chromosomes by chromosome painting in populations of Characidium to reveal their evolution, phylogeny, and biogeography. RESULTS: A W-specific probe for efficient chromosome painting was isolated by microdissection and degenerate oligonucleotide primed-polymerase chain reaction (DOP-PCR) amplification of W chromosomes from C. gomesi. The W probe generated weak signals dispersed on the proto sex chromosomes in C. zebra, dispersed signals in both W and Z chromosomes in C. lauroi and, in C. gomesi populations revealed a proximal site on the long arms of the Z chromosome and the entire W chromosome. All populations showed small terminal W probe sites in some autosomes. The 18S rDNA revealed distinctive patterns for each analyzed species/population with regard to proto sex chromosome, sex chromosome pair, and autosome location. CONCLUSIONS: The results from dual-color fluorescence in situ hybridization (dual-color FISH) using W and 18S rDNA probes allowed us to infer the putative evolutionary pathways for the differentiation of sex chromosomes and NORs, from structural rearrangements in a sex proto-chromosome, followed by gene erosion and heterochromatin amplification, morphological differentiation of the sex chromosomal pair, and NOR transposition, giving rise to the distinctive patterns observed among species/populations of Characidium. Biogeographic isolation and differentiation of sex chromosomes seem to have played a major role in the speciation process in this group of fish. BioMed Central 2011-07-25 /pmc/articles/PMC3160954/ /pubmed/21787398 http://dx.doi.org/10.1186/1471-2156-12-65 Text en Copyright ©2011 Machado et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Machado, Tatiana C Pansonato-Alves, José C Pucci, Marcela B Nogaroto, Viviane Almeida, Mara C Oliveira, Claudio Foresti, Fausto Bertollo, Luiz AC Moreira-Filho, Orlando Artoni, Roberto F Vicari, Marcelo R Chromosomal painting and ZW sex chromosomes differentiation in Characidium (Characiformes, Crenuchidae) |
title | Chromosomal painting and ZW sex chromosomes differentiation in Characidium (Characiformes, Crenuchidae) |
title_full | Chromosomal painting and ZW sex chromosomes differentiation in Characidium (Characiformes, Crenuchidae) |
title_fullStr | Chromosomal painting and ZW sex chromosomes differentiation in Characidium (Characiformes, Crenuchidae) |
title_full_unstemmed | Chromosomal painting and ZW sex chromosomes differentiation in Characidium (Characiformes, Crenuchidae) |
title_short | Chromosomal painting and ZW sex chromosomes differentiation in Characidium (Characiformes, Crenuchidae) |
title_sort | chromosomal painting and zw sex chromosomes differentiation in characidium (characiformes, crenuchidae) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3160954/ https://www.ncbi.nlm.nih.gov/pubmed/21787398 http://dx.doi.org/10.1186/1471-2156-12-65 |
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