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The effect of subconjunctival bevacizumab on corneal neovascularization, inflammation and re-epithelization in a rabbit model

PURPOSE: To evaluate the use of subconjunctival bevacizumab on corneal neovascularization in an experimental rabbit model for its effect on vessel extension, inflammation, and corneal epithelialization. METHODS: In this prospective, randomized, blinded, experimental study, 20 rabbits were submitted...

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Autores principales: Mello, Glauco Reggiani, Pizzolatti, Marcos Longo, Wasilewski, Daniel, Santhiago, Marcony R., Budel, Vinícius, Moreira, Hamilton
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3161226/
https://www.ncbi.nlm.nih.gov/pubmed/21915498
http://dx.doi.org/10.1590/S1807-59322011000800023
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author Mello, Glauco Reggiani
Pizzolatti, Marcos Longo
Wasilewski, Daniel
Santhiago, Marcony R.
Budel, Vinícius
Moreira, Hamilton
author_facet Mello, Glauco Reggiani
Pizzolatti, Marcos Longo
Wasilewski, Daniel
Santhiago, Marcony R.
Budel, Vinícius
Moreira, Hamilton
author_sort Mello, Glauco Reggiani
collection PubMed
description PURPOSE: To evaluate the use of subconjunctival bevacizumab on corneal neovascularization in an experimental rabbit model for its effect on vessel extension, inflammation, and corneal epithelialization. METHODS: In this prospective, randomized, blinded, experimental study, 20 rabbits were submitted to a chemical trauma with sodium hydroxide and subsequently divided into two groups. The experimental group received a subconjunctival injection of bevacizumab (0.15 m; 3.75 mg), and the control group received an injection of 0.15 ml saline solution. After 14 days, two blinded digital photograph analyses were conducted to evaluate the inflammation/diameter of the vessels according to pre-established criteria. A histopathological analysis of the cornea evaluated the state of the epithelium and the number of polymorphonuclear cells. RESULTS: A concordance analysis using Kappa's statistic showed a satisfactory level of agreement between the two blinded digital photography analyses. The neovascular vessel length was greater in the control group (p<0.01) than in the study group. However, the histopathological examination revealed no statistically significant differences between the groups in terms of the state of the epithelium and the number of polymorphonuclear cells. CONCLUSIONS: Subconjunctival bevacizumab inhibited neovascularization in the rabbit cornea. However, this drug was not effective at reducing inflammation. The drug did not induce persistent corneal epithelial defects.
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spelling pubmed-31612262011-08-29 The effect of subconjunctival bevacizumab on corneal neovascularization, inflammation and re-epithelization in a rabbit model Mello, Glauco Reggiani Pizzolatti, Marcos Longo Wasilewski, Daniel Santhiago, Marcony R. Budel, Vinícius Moreira, Hamilton Clinics (Sao Paulo) Basic Research PURPOSE: To evaluate the use of subconjunctival bevacizumab on corneal neovascularization in an experimental rabbit model for its effect on vessel extension, inflammation, and corneal epithelialization. METHODS: In this prospective, randomized, blinded, experimental study, 20 rabbits were submitted to a chemical trauma with sodium hydroxide and subsequently divided into two groups. The experimental group received a subconjunctival injection of bevacizumab (0.15 m; 3.75 mg), and the control group received an injection of 0.15 ml saline solution. After 14 days, two blinded digital photograph analyses were conducted to evaluate the inflammation/diameter of the vessels according to pre-established criteria. A histopathological analysis of the cornea evaluated the state of the epithelium and the number of polymorphonuclear cells. RESULTS: A concordance analysis using Kappa's statistic showed a satisfactory level of agreement between the two blinded digital photography analyses. The neovascular vessel length was greater in the control group (p<0.01) than in the study group. However, the histopathological examination revealed no statistically significant differences between the groups in terms of the state of the epithelium and the number of polymorphonuclear cells. CONCLUSIONS: Subconjunctival bevacizumab inhibited neovascularization in the rabbit cornea. However, this drug was not effective at reducing inflammation. The drug did not induce persistent corneal epithelial defects. Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo 2011-08 /pmc/articles/PMC3161226/ /pubmed/21915498 http://dx.doi.org/10.1590/S1807-59322011000800023 Text en Copyright © 2011 Hospital das Clínicas da FMUSP http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Basic Research
Mello, Glauco Reggiani
Pizzolatti, Marcos Longo
Wasilewski, Daniel
Santhiago, Marcony R.
Budel, Vinícius
Moreira, Hamilton
The effect of subconjunctival bevacizumab on corneal neovascularization, inflammation and re-epithelization in a rabbit model
title The effect of subconjunctival bevacizumab on corneal neovascularization, inflammation and re-epithelization in a rabbit model
title_full The effect of subconjunctival bevacizumab on corneal neovascularization, inflammation and re-epithelization in a rabbit model
title_fullStr The effect of subconjunctival bevacizumab on corneal neovascularization, inflammation and re-epithelization in a rabbit model
title_full_unstemmed The effect of subconjunctival bevacizumab on corneal neovascularization, inflammation and re-epithelization in a rabbit model
title_short The effect of subconjunctival bevacizumab on corneal neovascularization, inflammation and re-epithelization in a rabbit model
title_sort effect of subconjunctival bevacizumab on corneal neovascularization, inflammation and re-epithelization in a rabbit model
topic Basic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3161226/
https://www.ncbi.nlm.nih.gov/pubmed/21915498
http://dx.doi.org/10.1590/S1807-59322011000800023
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