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Serum Messenger RNA as a Biomarker and its Clinical Usefulness in Malignancies

A number of biomarkers are used clinically and many protein-based assay methods are available. Improvements in the method to utilize specific antibodies have led to remarkable progress in clinical diagnosis using biomarkers. Proteomics studies to identify better biomarkers have been performed worldw...

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Detalles Bibliográficos
Autores principales: Miura, Norimasa, Hasegawa, Junichi, Shiota, Goshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Libertas Academica 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3161670/
https://www.ncbi.nlm.nih.gov/pubmed/21892326
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author Miura, Norimasa
Hasegawa, Junichi
Shiota, Goshi
author_facet Miura, Norimasa
Hasegawa, Junichi
Shiota, Goshi
author_sort Miura, Norimasa
collection PubMed
description A number of biomarkers are used clinically and many protein-based assay methods are available. Improvements in the method to utilize specific antibodies have led to remarkable progress in clinical diagnosis using biomarkers. Proteomics studies to identify better biomarkers have been performed worldwide by using a protein-based comprehensive method. The detection rate of conventional biomarkers can not improve further. Now is a time that a breakthrough is needed. We previously proposed mRNA, which is circulating in the body, as a novel material for biomarkers. mRNA is an unexpectedly useful molecule, not only because it can detect genes with a low expression level in protein, but also because it can detect the expression from non-coding RNA precursor genes or gene products with limited secretion from the cells. Circulating mRNA has been thought to be unstable in blood containing RNase. We confirm that mRNA remains at the same level for 24 hours after blood sampling. Unlike DNA, the RNA molecule can reflect events in the human body which occurred within a day, resulting in an early diagnosis of diseases. We report the possibility to detect and quantify cancer-derived mRNAs circulating in human vessels. We introduce the detection of serum mRNA as a useful biomarker of human malignancies.
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spelling pubmed-31616702011-09-02 Serum Messenger RNA as a Biomarker and its Clinical Usefulness in Malignancies Miura, Norimasa Hasegawa, Junichi Shiota, Goshi Clin Med Oncol Review A number of biomarkers are used clinically and many protein-based assay methods are available. Improvements in the method to utilize specific antibodies have led to remarkable progress in clinical diagnosis using biomarkers. Proteomics studies to identify better biomarkers have been performed worldwide by using a protein-based comprehensive method. The detection rate of conventional biomarkers can not improve further. Now is a time that a breakthrough is needed. We previously proposed mRNA, which is circulating in the body, as a novel material for biomarkers. mRNA is an unexpectedly useful molecule, not only because it can detect genes with a low expression level in protein, but also because it can detect the expression from non-coding RNA precursor genes or gene products with limited secretion from the cells. Circulating mRNA has been thought to be unstable in blood containing RNase. We confirm that mRNA remains at the same level for 24 hours after blood sampling. Unlike DNA, the RNA molecule can reflect events in the human body which occurred within a day, resulting in an early diagnosis of diseases. We report the possibility to detect and quantify cancer-derived mRNAs circulating in human vessels. We introduce the detection of serum mRNA as a useful biomarker of human malignancies. Libertas Academica 2008-09-22 /pmc/articles/PMC3161670/ /pubmed/21892326 Text en © 2008 the author(s), publisher and licensee Libertas Academica Ltd. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license http://creativecommons.org/licenses/by/3.0/).
spellingShingle Review
Miura, Norimasa
Hasegawa, Junichi
Shiota, Goshi
Serum Messenger RNA as a Biomarker and its Clinical Usefulness in Malignancies
title Serum Messenger RNA as a Biomarker and its Clinical Usefulness in Malignancies
title_full Serum Messenger RNA as a Biomarker and its Clinical Usefulness in Malignancies
title_fullStr Serum Messenger RNA as a Biomarker and its Clinical Usefulness in Malignancies
title_full_unstemmed Serum Messenger RNA as a Biomarker and its Clinical Usefulness in Malignancies
title_short Serum Messenger RNA as a Biomarker and its Clinical Usefulness in Malignancies
title_sort serum messenger rna as a biomarker and its clinical usefulness in malignancies
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3161670/
https://www.ncbi.nlm.nih.gov/pubmed/21892326
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