Cargando…

A One-Step Real-Time Multiplex PCR for Screening Y-Chromosomal Microdeletions without Downstream Amplicon Size Analysis

BACKGOUND: Y-chromosomal microdeletions (YCMD) are one of the major genetic causes for non-obstructive azoospermia. Genetic testing for YCMD by multiplex polymerase chain reaction (PCR) is an established method for quick and robust screening of deletions in the AZF regions of the Y-chromosome. Multi...

Descripción completa

Detalles Bibliográficos
Autores principales:	Kozina, Viviana, Cappallo-Obermann, Heike, Gromoll, Jörg, Spiess, Andrej-Nikolai
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2011
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3161745/ https://www.ncbi.nlm.nih.gov/pubmed/21887237 http://dx.doi.org/10.1371/journal.pone.0023174

Ejemplares similares

A biopsy sample reduction approach to identify significant alterations of the testicular transcriptome in the presence of Y-chromosomal microdeletions that are independent of germ cell composition
por: Cappallo-Obermann, Heike, et al.
Publicado: (2010)

Multiplex PCR Screening of Y-chromosome microdeletions in azoospermic ICSI candidate men
por: Sheikhha, Mohammad Hasan, et al.
Publicado: (2013)

An Economical and Flexible Dual Barcoding, Two-Step PCR Approach for Highly Multiplexed Amplicon Sequencing
por: Pjevac, Petra, et al.
Publicado: (2021)

Multiplex PCR Targeted Amplicon Sequencing (MTA-Seq): Simple, Flexible, and Versatile SNP Genotyping by Highly Multiplexed PCR Amplicon Sequencing
por: Onda, Yoshihiko, et al.
Publicado: (2018)

Targeted RNA-Sequencing with Competitive Multiplex-PCR Amplicon Libraries
por: Blomquist, Thomas M., et al.
Publicado: (2013)

Multiplex PCR based screening for microdeletions in azoospermia factor region of Y chromosome in azoospermic and severe oligozoospermic south Indian men
por: Suganthi, Ramaswamy, et al.
Publicado: (2013)

Correction: Targeted RNA-Sequencing with Competitive Multiplex-PCR Amplicon Libraries
por: Blomquist, Thomas M., et al.
Publicado: (2013)

Radiation Rescue: Mesenchymal Stromal Cells Protect from Lethal Irradiation
por: Lange, Claudia, et al.
Publicado: (2011)

Simulate_PCR for amplicon prediction and annotation from multiplex, degenerate primers and probes
por: Gardner, Shea N, et al.
Publicado: (2014)

qPCR data analysis: Better results through iconoclasm
por: Tellinghuisen, Joel, et al.
Publicado: (2019)

A multiplex PCR amplicon sequencing assay to screen genetic hearing loss variants in newborns
por: Yang, Haiyan, et al.
Publicado: (2021)

Examination of Y-Chromosomal Microdeletions and Partial Microdeletions in Idiopathic Infertility in East Hungarian Patients
por: Mokánszki, Attila, et al.
Publicado: (2018)

Prediction of smoking by multiplex bisulfite PCR with long amplicons considering allele-specific effects on DNA methylation
por: Kondratyev, Nikolay, et al.
Publicado: (2018)

Chromosome 15q24 microdeletion syndrome
por: Magoulas, Pilar L, et al.
Publicado: (2012)

Chromosomal abnormalities and Y chromosome microdeletions in infertile men from Morocco
por: Naasse, Yassine, et al.
Publicado: (2015)

Correction to: Prediction of smoking by multiplex bisulfite PCR with long amplicons considering allele-specific effects on DNA methylation
por: Kondratyev, Nikolay, et al.
Publicado: (2018)

SESAME (SEquence Sorter & AMplicon Explorer): genotyping based on high-throughput multiplex amplicon sequencing
por: Meglécz, Emese, et al.
Publicado: (2011)

Highly accurate sigmoidal fitting of real-time PCR data by introducing a parameter for asymmetry
por: Spiess, Andrej-Nikolai, et al.
Publicado: (2008)

Discrepancy in the results of Y chromosome microdeletions in an Iranian population
por: Saliminejad, Kioomars, et al.
Publicado: (2011)

Screening of ‘Y’ chromosome microdeletions in Iranian infertile males
por: Malekasgar, Ali Mohammad, et al.
Publicado: (2008)

The prevalence of Y chromosome microdeletions in Pakistani infertile men
por: Tabassum Siddiqui, Rubina, et al.
Publicado: (2013)

Y-chromosome microdeletions in nonobstructive azoospermia and severe oligozoospermia
por: Gonçalves, Carolina, et al.
Publicado: (2017)

Accurate multiplexing and filtering for high-throughput amplicon-sequencing
por: Esling, Philippe, et al.
Publicado: (2015)

Direct Metatranscriptome RNA-seq and Multiplex RT-PCR Amplicon Sequencing on Nanopore MinION – Promising Strategies for Multiplex Identification of Viable Pathogens in Food
por: Yang, Manyun, et al.
Publicado: (2020)

Multiplexed ddPCR-amplicon sequencing reveals isolated Plasmodium falciparum populations amenable to local elimination in Zanzibar, Tanzania
por: Holzschuh, Aurel, et al.
Publicado: (2023)

Using Cy5-dUTP labelling of RPA-amplicons with downstream microarray analysis for the detection of antibiotic resistance genes
por: Warmt, Christian, et al.
Publicado: (2021)

Y chromosome microdeletions in infertile men with idiopathic oligo- or azoospermia
por: Hellani, Ali, et al.
Publicado: (2006)

The Association between Y Chromosome Microdeletion and Recurrent Pregnancy Loss
por: Ghorbian, S, et al.
Publicado: (2012)

High Prevalence of Y Chromosome Partial Microdeletions in Overweight Men
por: Biabangard zak, Atefeh, et al.
Publicado: (2015)

Microdeletion of Y chromosome as a cause of recurrent pregnancy loss
por: Agarwal, Shubhra, et al.
Publicado: (2015)

Y chromosome microdeletions frequency in idiopathic azoospermia, oligoasthenozoospermia, and oligospermia
por: Gholami, Delnya, et al.
Publicado: (2017)

Histology and sperm retrieval among men with Y chromosome microdeletions
por: Yuen, Wallace, et al.
Publicado: (2021)

Prevalence of Y chromosome microdeletions among infertile Mongolian men
por: Damdinsuren, Erdenesuvd, et al.
Publicado: (2022)

Prenatal Diagnosis of Chromosome 16p11.2 Microdeletion
por: Wang, You, et al.
Publicado: (2022)

Microdeletions and vertical transmission of the Y-chromosome azoospermia factor region
por: Deng, Chen-Yao, et al.
Publicado: (2022)

Simultaneous multiplexed amplicon sequencing and transcriptome profiling in single cells
por: Saikia, Mridusmita, et al.
Publicado: (2018)

Chromosomal abnormalities and Y chromosome microdeletions in infertile men with azoospermia and oligozoospermia in Eastern China
por: Sha, Jing, et al.
Publicado: (2019)

Chromosome 22q11.2 microdeletion in monozygotic twins with discordant phenotype and deletion size
por: Halder, Ashutosh, et al.
Publicado: (2012)

Using Multiplex Amplicon PCR Technology to Efficiently and Timely Generate Rift Valley Fever Virus Sequence Data for Genomic Surveillance
por: Juma, John, et al.
Publicado: (2023)

SMAP design: a multiplex PCR amplicon and gRNA design tool to screen for natural and CRISPR-induced genetic variation
por: Develtere, Ward, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_u5evuv6d34830beoakrkfvldgk