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Analysis of regulation of pentose utilisation in Aspergillus niger reveals evolutionary adaptations in Eurotiales
Aspergilli are commonly found in soil and on decaying plant material. D-xylose and L-arabinose are highly abundant components of plant biomass. They are released from polysaccharides by fungi using a set of extracellular enzymes and subsequently converted intracellularly through the pentose cataboli...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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CBS Fungal Biodiversity Centre
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3161754/ https://www.ncbi.nlm.nih.gov/pubmed/21892241 http://dx.doi.org/10.3114/sim.2011.69.03 |
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author | Battaglia, E. Visser, L. Nijssen, A. van Veluw, G.J. Wösten, H.A.B. de Vries, R.P. |
author_facet | Battaglia, E. Visser, L. Nijssen, A. van Veluw, G.J. Wösten, H.A.B. de Vries, R.P. |
author_sort | Battaglia, E. |
collection | PubMed |
description | Aspergilli are commonly found in soil and on decaying plant material. D-xylose and L-arabinose are highly abundant components of plant biomass. They are released from polysaccharides by fungi using a set of extracellular enzymes and subsequently converted intracellularly through the pentose catabolic pathway (PCP). In this study, the L-arabinose responsive transcriptional activator (AraR) is identified in Aspergillus niger and was shown to control the L-arabinose catabolic pathway as well as expression of genes encoding extracellular L-arabinose releasing enzymes. AraR interacts with the D-xylose-responsive transcriptional activator XlnR in the regulation of the pentose catabolic pathway, but not with respect to release of L-arabinose and D-xylose. AraR was only identified in the Eurotiales, more specifically in the family Trichocomaceae and appears to have originated from a gene duplication event (from XlnR) after this order or family split from the other filamentous ascomycetes. XlnR is present in all filamentous ascomycetes with the exception of members of the Onygenales. Since the Onygenales and Eurotiales are both part of the subclass Eurotiomycetidae, this indicates that strong adaptation of the regulation of pentose utilisation has occurred at this evolutionary node. In Eurotiales a unique two-component regulatory system for pentose release and metabolism has evolved, while the regulatory system was lost in the Onygenales. The observed evolutionary changes (in Eurotiomycetidae) mainly affect the regulatory system as in contrast, homologues for most genes of the L-arabinose/D-xylose catabolic pathway are present in all the filamentous fungi, irrespective of the presence of XlnR and/or AraR. |
format | Online Article Text |
id | pubmed-3161754 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | CBS Fungal Biodiversity Centre |
record_format | MEDLINE/PubMed |
spelling | pubmed-31617542011-09-02 Analysis of regulation of pentose utilisation in Aspergillus niger reveals evolutionary adaptations in Eurotiales Battaglia, E. Visser, L. Nijssen, A. van Veluw, G.J. Wösten, H.A.B. de Vries, R.P. Stud Mycol Articles Aspergilli are commonly found in soil and on decaying plant material. D-xylose and L-arabinose are highly abundant components of plant biomass. They are released from polysaccharides by fungi using a set of extracellular enzymes and subsequently converted intracellularly through the pentose catabolic pathway (PCP). In this study, the L-arabinose responsive transcriptional activator (AraR) is identified in Aspergillus niger and was shown to control the L-arabinose catabolic pathway as well as expression of genes encoding extracellular L-arabinose releasing enzymes. AraR interacts with the D-xylose-responsive transcriptional activator XlnR in the regulation of the pentose catabolic pathway, but not with respect to release of L-arabinose and D-xylose. AraR was only identified in the Eurotiales, more specifically in the family Trichocomaceae and appears to have originated from a gene duplication event (from XlnR) after this order or family split from the other filamentous ascomycetes. XlnR is present in all filamentous ascomycetes with the exception of members of the Onygenales. Since the Onygenales and Eurotiales are both part of the subclass Eurotiomycetidae, this indicates that strong adaptation of the regulation of pentose utilisation has occurred at this evolutionary node. In Eurotiales a unique two-component regulatory system for pentose release and metabolism has evolved, while the regulatory system was lost in the Onygenales. The observed evolutionary changes (in Eurotiomycetidae) mainly affect the regulatory system as in contrast, homologues for most genes of the L-arabinose/D-xylose catabolic pathway are present in all the filamentous fungi, irrespective of the presence of XlnR and/or AraR. CBS Fungal Biodiversity Centre 2011-06-30 /pmc/articles/PMC3161754/ /pubmed/21892241 http://dx.doi.org/10.3114/sim.2011.69.03 Text en Copyright 2011 CBS-KNAW Fungal Biodiversity Centre You are free to share - to copy, distribute and transmit the work, under the following conditions: Attribution: You must attribute the work in the manner specified by the author or licensor (but not in any way that suggests that they endorse you or your use of the work). Non-commercial: You may not use this work for commercial purposes. No derivative works: You may not alter, transform, or build upon this work. For any reuse or distribution, you must make clear to others the license terms of this work, which can be found at http://creativecommons.org/licenses/by-nc-nd/3.0/legalcode. Any of the above conditions can be waived if you get permission from the copyright holder. Nothing in this license impairs or restricts the author's moral rights. |
spellingShingle | Articles Battaglia, E. Visser, L. Nijssen, A. van Veluw, G.J. Wösten, H.A.B. de Vries, R.P. Analysis of regulation of pentose utilisation in Aspergillus niger reveals evolutionary adaptations in Eurotiales |
title | Analysis of regulation of pentose utilisation in Aspergillus niger reveals evolutionary adaptations in Eurotiales |
title_full | Analysis of regulation of pentose utilisation in Aspergillus niger reveals evolutionary adaptations in Eurotiales |
title_fullStr | Analysis of regulation of pentose utilisation in Aspergillus niger reveals evolutionary adaptations in Eurotiales |
title_full_unstemmed | Analysis of regulation of pentose utilisation in Aspergillus niger reveals evolutionary adaptations in Eurotiales |
title_short | Analysis of regulation of pentose utilisation in Aspergillus niger reveals evolutionary adaptations in Eurotiales |
title_sort | analysis of regulation of pentose utilisation in aspergillus niger reveals evolutionary adaptations in eurotiales |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3161754/ https://www.ncbi.nlm.nih.gov/pubmed/21892241 http://dx.doi.org/10.3114/sim.2011.69.03 |
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