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Seamless Gene Tagging by Endonuclease-Driven Homologous Recombination
Gene tagging facilitates systematic genomic and proteomic analyses but chromosomal tagging typically disrupts gene regulatory sequences. Here we describe a seamless gene tagging approach that preserves endogenous gene regulation and is potentially applicable in any species with efficient DNA double-...
Autores principales: | Khmelinskii, Anton, Meurer, Matthias, Duishoev, Nurlanbek, Delhomme, Nicolas, Knop, Michael |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3161820/ https://www.ncbi.nlm.nih.gov/pubmed/21915245 http://dx.doi.org/10.1371/journal.pone.0023794 |
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