Cargando…

Global Chromosomal Structural Instability in a Subpopulation of Starving Escherichia coli Cells

Copy-number variations (CNVs) constitute very common differences between individual humans and possibly all genomes and may therefore be important fuel for evolution, yet how they form remains elusive. In starving Escherichia coli, gene amplification is induced by stress, controlled by the general s...

Descripción completa

Detalles Bibliográficos
Autores principales: Lin, Dongxu, Gibson, Ian B., Moore, Jessica M., Thornton, P. C., Leal, Suzanne M., Hastings, P. J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3161906/
https://www.ncbi.nlm.nih.gov/pubmed/21901104
http://dx.doi.org/10.1371/journal.pgen.1002223
_version_ 1782210754317910016
author Lin, Dongxu
Gibson, Ian B.
Moore, Jessica M.
Thornton, P. C.
Leal, Suzanne M.
Hastings, P. J.
author_facet Lin, Dongxu
Gibson, Ian B.
Moore, Jessica M.
Thornton, P. C.
Leal, Suzanne M.
Hastings, P. J.
author_sort Lin, Dongxu
collection PubMed
description Copy-number variations (CNVs) constitute very common differences between individual humans and possibly all genomes and may therefore be important fuel for evolution, yet how they form remains elusive. In starving Escherichia coli, gene amplification is induced by stress, controlled by the general stress response. Amplification has been detected only encompassing genes that confer a growth advantage when amplified. We studied the structure of stress-induced gene amplification in starving cells in the Lac assay in Escherichia coli by array comparative genomic hybridization (aCGH), with polymerase chain reaction (pcr) and DNA sequencing to establish the structures generated. About 10% of 300 amplified isolates carried other chromosomal structural change in addition to amplification. Most of these were inversions and duplications associated with the amplification event. This complexity supports a mechanism similar to that seen in human non-recurrent copy number variants. We interpret these complex events in terms of repeated template switching during DNA replication. Importantly, we found a significant occurrence (6 out of 300) of chromosomal structural changes that were apparently not involved in the amplification event. These secondary changes were absent from 240 samples derived from starved cells not carrying amplification, suggesting that amplification happens in a differentiated subpopulation of stressed cells licensed for global chromosomal structural change and genomic instability. These data imply that chromosomal structural changes occur in bursts or showers of instability that may have the potential to drive rapid evolution.
format Online
Article
Text
id pubmed-3161906
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-31619062011-09-07 Global Chromosomal Structural Instability in a Subpopulation of Starving Escherichia coli Cells Lin, Dongxu Gibson, Ian B. Moore, Jessica M. Thornton, P. C. Leal, Suzanne M. Hastings, P. J. PLoS Genet Research Article Copy-number variations (CNVs) constitute very common differences between individual humans and possibly all genomes and may therefore be important fuel for evolution, yet how they form remains elusive. In starving Escherichia coli, gene amplification is induced by stress, controlled by the general stress response. Amplification has been detected only encompassing genes that confer a growth advantage when amplified. We studied the structure of stress-induced gene amplification in starving cells in the Lac assay in Escherichia coli by array comparative genomic hybridization (aCGH), with polymerase chain reaction (pcr) and DNA sequencing to establish the structures generated. About 10% of 300 amplified isolates carried other chromosomal structural change in addition to amplification. Most of these were inversions and duplications associated with the amplification event. This complexity supports a mechanism similar to that seen in human non-recurrent copy number variants. We interpret these complex events in terms of repeated template switching during DNA replication. Importantly, we found a significant occurrence (6 out of 300) of chromosomal structural changes that were apparently not involved in the amplification event. These secondary changes were absent from 240 samples derived from starved cells not carrying amplification, suggesting that amplification happens in a differentiated subpopulation of stressed cells licensed for global chromosomal structural change and genomic instability. These data imply that chromosomal structural changes occur in bursts or showers of instability that may have the potential to drive rapid evolution. Public Library of Science 2011-08-25 /pmc/articles/PMC3161906/ /pubmed/21901104 http://dx.doi.org/10.1371/journal.pgen.1002223 Text en Lin et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Lin, Dongxu
Gibson, Ian B.
Moore, Jessica M.
Thornton, P. C.
Leal, Suzanne M.
Hastings, P. J.
Global Chromosomal Structural Instability in a Subpopulation of Starving Escherichia coli Cells
title Global Chromosomal Structural Instability in a Subpopulation of Starving Escherichia coli Cells
title_full Global Chromosomal Structural Instability in a Subpopulation of Starving Escherichia coli Cells
title_fullStr Global Chromosomal Structural Instability in a Subpopulation of Starving Escherichia coli Cells
title_full_unstemmed Global Chromosomal Structural Instability in a Subpopulation of Starving Escherichia coli Cells
title_short Global Chromosomal Structural Instability in a Subpopulation of Starving Escherichia coli Cells
title_sort global chromosomal structural instability in a subpopulation of starving escherichia coli cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3161906/
https://www.ncbi.nlm.nih.gov/pubmed/21901104
http://dx.doi.org/10.1371/journal.pgen.1002223
work_keys_str_mv AT lindongxu globalchromosomalstructuralinstabilityinasubpopulationofstarvingescherichiacolicells
AT gibsonianb globalchromosomalstructuralinstabilityinasubpopulationofstarvingescherichiacolicells
AT moorejessicam globalchromosomalstructuralinstabilityinasubpopulationofstarvingescherichiacolicells
AT thorntonpc globalchromosomalstructuralinstabilityinasubpopulationofstarvingescherichiacolicells
AT lealsuzannem globalchromosomalstructuralinstabilityinasubpopulationofstarvingescherichiacolicells
AT hastingspj globalchromosomalstructuralinstabilityinasubpopulationofstarvingescherichiacolicells