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MrkH, a Novel c-di-GMP-Dependent Transcriptional Activator, Controls Klebsiella pneumoniae Biofilm Formation by Regulating Type 3 Fimbriae Expression

Klebsiella pneumoniae causes significant morbidity and mortality worldwide, particularly amongst hospitalized individuals. The principle mechanism for pathogenesis in hospital environments involves the formation of biofilms, primarily on implanted medical devices. In this study, we constructed a tra...

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Autores principales: Wilksch, Jonathan J., Yang, Ji, Clements, Abigail, Gabbe, Jacinta L., Short, Kirsty R., Cao, Hanwei, Cavaliere, Rosalia, James, Catherine E., Whitchurch, Cynthia B., Schembri, Mark A., Chuah, Mary L. C., Liang, Zhao-Xun, Wijburg, Odilia L., Jenney, Adam W., Lithgow, Trevor, Strugnell, Richard A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3161979/
https://www.ncbi.nlm.nih.gov/pubmed/21901098
http://dx.doi.org/10.1371/journal.ppat.1002204
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author Wilksch, Jonathan J.
Yang, Ji
Clements, Abigail
Gabbe, Jacinta L.
Short, Kirsty R.
Cao, Hanwei
Cavaliere, Rosalia
James, Catherine E.
Whitchurch, Cynthia B.
Schembri, Mark A.
Chuah, Mary L. C.
Liang, Zhao-Xun
Wijburg, Odilia L.
Jenney, Adam W.
Lithgow, Trevor
Strugnell, Richard A.
author_facet Wilksch, Jonathan J.
Yang, Ji
Clements, Abigail
Gabbe, Jacinta L.
Short, Kirsty R.
Cao, Hanwei
Cavaliere, Rosalia
James, Catherine E.
Whitchurch, Cynthia B.
Schembri, Mark A.
Chuah, Mary L. C.
Liang, Zhao-Xun
Wijburg, Odilia L.
Jenney, Adam W.
Lithgow, Trevor
Strugnell, Richard A.
author_sort Wilksch, Jonathan J.
collection PubMed
description Klebsiella pneumoniae causes significant morbidity and mortality worldwide, particularly amongst hospitalized individuals. The principle mechanism for pathogenesis in hospital environments involves the formation of biofilms, primarily on implanted medical devices. In this study, we constructed a transposon mutant library in a clinical isolate, K. pneumoniae AJ218, to identify the genes and pathways implicated in biofilm formation. Three mutants severely defective in biofilm formation contained insertions within the mrkABCDF genes encoding the main structural subunit and assembly machinery for type 3 fimbriae. Two other mutants carried insertions within the yfiN and mrkJ genes, which encode GGDEF domain- and EAL domain-containing c-di-GMP turnover enzymes, respectively. The remaining two isolates contained insertions that inactivated the mrkH and mrkI genes, which encode for novel proteins with a c-di-GMP-binding PilZ domain and a LuxR-type transcriptional regulator, respectively. Biochemical and functional assays indicated that the effects of these factors on biofilm formation accompany concomitant changes in type 3 fimbriae expression. We mapped the transcriptional start site of mrkA, demonstrated that MrkH directly activates transcription of the mrkA promoter and showed that MrkH binds strongly to the mrkA regulatory region only in the presence of c-di-GMP. Furthermore, a point mutation in the putative c-di-GMP-binding domain of MrkH completely abolished its function as a transcriptional activator. In vivo analysis of the yfiN and mrkJ genes strongly indicated their c-di-GMP-specific function as diguanylate cyclase and phosphodiesterase, respectively. In addition, in vitro assays showed that purified MrkJ protein has strong c-di-GMP phosphodiesterase activity. These results demonstrate for the first time that c-di-GMP can function as an effector to stimulate the activity of a transcriptional activator, and explain how type 3 fimbriae expression is coordinated with other gene expression programs in K. pneumoniae to promote biofilm formation to implanted medical devices.
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spelling pubmed-31619792011-09-07 MrkH, a Novel c-di-GMP-Dependent Transcriptional Activator, Controls Klebsiella pneumoniae Biofilm Formation by Regulating Type 3 Fimbriae Expression Wilksch, Jonathan J. Yang, Ji Clements, Abigail Gabbe, Jacinta L. Short, Kirsty R. Cao, Hanwei Cavaliere, Rosalia James, Catherine E. Whitchurch, Cynthia B. Schembri, Mark A. Chuah, Mary L. C. Liang, Zhao-Xun Wijburg, Odilia L. Jenney, Adam W. Lithgow, Trevor Strugnell, Richard A. PLoS Pathog Research Article Klebsiella pneumoniae causes significant morbidity and mortality worldwide, particularly amongst hospitalized individuals. The principle mechanism for pathogenesis in hospital environments involves the formation of biofilms, primarily on implanted medical devices. In this study, we constructed a transposon mutant library in a clinical isolate, K. pneumoniae AJ218, to identify the genes and pathways implicated in biofilm formation. Three mutants severely defective in biofilm formation contained insertions within the mrkABCDF genes encoding the main structural subunit and assembly machinery for type 3 fimbriae. Two other mutants carried insertions within the yfiN and mrkJ genes, which encode GGDEF domain- and EAL domain-containing c-di-GMP turnover enzymes, respectively. The remaining two isolates contained insertions that inactivated the mrkH and mrkI genes, which encode for novel proteins with a c-di-GMP-binding PilZ domain and a LuxR-type transcriptional regulator, respectively. Biochemical and functional assays indicated that the effects of these factors on biofilm formation accompany concomitant changes in type 3 fimbriae expression. We mapped the transcriptional start site of mrkA, demonstrated that MrkH directly activates transcription of the mrkA promoter and showed that MrkH binds strongly to the mrkA regulatory region only in the presence of c-di-GMP. Furthermore, a point mutation in the putative c-di-GMP-binding domain of MrkH completely abolished its function as a transcriptional activator. In vivo analysis of the yfiN and mrkJ genes strongly indicated their c-di-GMP-specific function as diguanylate cyclase and phosphodiesterase, respectively. In addition, in vitro assays showed that purified MrkJ protein has strong c-di-GMP phosphodiesterase activity. These results demonstrate for the first time that c-di-GMP can function as an effector to stimulate the activity of a transcriptional activator, and explain how type 3 fimbriae expression is coordinated with other gene expression programs in K. pneumoniae to promote biofilm formation to implanted medical devices. Public Library of Science 2011-08-25 /pmc/articles/PMC3161979/ /pubmed/21901098 http://dx.doi.org/10.1371/journal.ppat.1002204 Text en Wilksch et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Wilksch, Jonathan J.
Yang, Ji
Clements, Abigail
Gabbe, Jacinta L.
Short, Kirsty R.
Cao, Hanwei
Cavaliere, Rosalia
James, Catherine E.
Whitchurch, Cynthia B.
Schembri, Mark A.
Chuah, Mary L. C.
Liang, Zhao-Xun
Wijburg, Odilia L.
Jenney, Adam W.
Lithgow, Trevor
Strugnell, Richard A.
MrkH, a Novel c-di-GMP-Dependent Transcriptional Activator, Controls Klebsiella pneumoniae Biofilm Formation by Regulating Type 3 Fimbriae Expression
title MrkH, a Novel c-di-GMP-Dependent Transcriptional Activator, Controls Klebsiella pneumoniae Biofilm Formation by Regulating Type 3 Fimbriae Expression
title_full MrkH, a Novel c-di-GMP-Dependent Transcriptional Activator, Controls Klebsiella pneumoniae Biofilm Formation by Regulating Type 3 Fimbriae Expression
title_fullStr MrkH, a Novel c-di-GMP-Dependent Transcriptional Activator, Controls Klebsiella pneumoniae Biofilm Formation by Regulating Type 3 Fimbriae Expression
title_full_unstemmed MrkH, a Novel c-di-GMP-Dependent Transcriptional Activator, Controls Klebsiella pneumoniae Biofilm Formation by Regulating Type 3 Fimbriae Expression
title_short MrkH, a Novel c-di-GMP-Dependent Transcriptional Activator, Controls Klebsiella pneumoniae Biofilm Formation by Regulating Type 3 Fimbriae Expression
title_sort mrkh, a novel c-di-gmp-dependent transcriptional activator, controls klebsiella pneumoniae biofilm formation by regulating type 3 fimbriae expression
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3161979/
https://www.ncbi.nlm.nih.gov/pubmed/21901098
http://dx.doi.org/10.1371/journal.ppat.1002204
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