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Histo-Blood Group Antigens Act as Attachment Factors of Rabbit Hemorrhagic Disease Virus Infection in a Virus Strain-Dependent Manner

Rabbit Hemorrhagic disease virus (RHDV), a calicivirus of the Lagovirus genus, and responsible for rabbit hemorrhagic disease (RHD), kills rabbits between 48 to 72 hours post infection with mortality rates as high as 50–90%. Caliciviruses, including noroviruses and RHDV, have been shown to bind hist...

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Autores principales: Nyström, Kristina, Le Gall-Reculé, Ghislaine, Grassi, Paola, Abrantes, Joana, Ruvoën-Clouet, Nathalie, Le Moullac-Vaidye, Beatrice, Lopes, Ana M., Esteves, Pedro J., Strive, Tanja, Marchandeau, Stéphane, Dell, Anne, Haslam, Stuart M., Le Pendu, Jacques
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3161982/
https://www.ncbi.nlm.nih.gov/pubmed/21901093
http://dx.doi.org/10.1371/journal.ppat.1002188
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author Nyström, Kristina
Le Gall-Reculé, Ghislaine
Grassi, Paola
Abrantes, Joana
Ruvoën-Clouet, Nathalie
Le Moullac-Vaidye, Beatrice
Lopes, Ana M.
Esteves, Pedro J.
Strive, Tanja
Marchandeau, Stéphane
Dell, Anne
Haslam, Stuart M.
Le Pendu, Jacques
author_facet Nyström, Kristina
Le Gall-Reculé, Ghislaine
Grassi, Paola
Abrantes, Joana
Ruvoën-Clouet, Nathalie
Le Moullac-Vaidye, Beatrice
Lopes, Ana M.
Esteves, Pedro J.
Strive, Tanja
Marchandeau, Stéphane
Dell, Anne
Haslam, Stuart M.
Le Pendu, Jacques
author_sort Nyström, Kristina
collection PubMed
description Rabbit Hemorrhagic disease virus (RHDV), a calicivirus of the Lagovirus genus, and responsible for rabbit hemorrhagic disease (RHD), kills rabbits between 48 to 72 hours post infection with mortality rates as high as 50–90%. Caliciviruses, including noroviruses and RHDV, have been shown to bind histo-blood group antigens (HBGA) and human non-secretor individuals lacking ABH antigens in epithelia have been found to be resistant to norovirus infection. RHDV virus-like particles have previously been shown to bind the H type 2 and A antigens. In this study we present a comprehensive assessment of the strain-specific binding patterns of different RHDV isolates to HBGAs. We characterized the HBGA expression in the duodenum of wild and domestic rabbits by mass spectrometry and relative quantification of A, B and H type 2 expression. A detailed binding analysis of a range of RHDV strains, to synthetic sugars and human red blood cells, as well as to rabbit duodenum, a likely gastrointestinal site for viral entrance was performed. Enzymatic cleavage of HBGA epitopes confirmed binding specificity. Binding was observed to blood group B, A and H type 2 epitopes in a strain-dependent manner with slight differences in specificity for A, B or H epitopes allowing RHDV strains to preferentially recognize different subgroups of animals. Strains related to the earliest described RHDV outbreak were not able to bind A, whereas all other genotypes have acquired A binding. In an experimental infection study, rabbits lacking the correct HBGA ligands were resistant to lethal RHDV infection at low challenge doses. Similarly, survivors of outbreaks in wild populations showed increased frequency of weak binding phenotypes, indicating selection for host resistance depending on the strain circulating in the population. HBGAs thus act as attachment factors facilitating infection, while their polymorphism of expression could contribute to generate genetic resistance to RHDV at the population level.
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spelling pubmed-31619822011-09-07 Histo-Blood Group Antigens Act as Attachment Factors of Rabbit Hemorrhagic Disease Virus Infection in a Virus Strain-Dependent Manner Nyström, Kristina Le Gall-Reculé, Ghislaine Grassi, Paola Abrantes, Joana Ruvoën-Clouet, Nathalie Le Moullac-Vaidye, Beatrice Lopes, Ana M. Esteves, Pedro J. Strive, Tanja Marchandeau, Stéphane Dell, Anne Haslam, Stuart M. Le Pendu, Jacques PLoS Pathog Research Article Rabbit Hemorrhagic disease virus (RHDV), a calicivirus of the Lagovirus genus, and responsible for rabbit hemorrhagic disease (RHD), kills rabbits between 48 to 72 hours post infection with mortality rates as high as 50–90%. Caliciviruses, including noroviruses and RHDV, have been shown to bind histo-blood group antigens (HBGA) and human non-secretor individuals lacking ABH antigens in epithelia have been found to be resistant to norovirus infection. RHDV virus-like particles have previously been shown to bind the H type 2 and A antigens. In this study we present a comprehensive assessment of the strain-specific binding patterns of different RHDV isolates to HBGAs. We characterized the HBGA expression in the duodenum of wild and domestic rabbits by mass spectrometry and relative quantification of A, B and H type 2 expression. A detailed binding analysis of a range of RHDV strains, to synthetic sugars and human red blood cells, as well as to rabbit duodenum, a likely gastrointestinal site for viral entrance was performed. Enzymatic cleavage of HBGA epitopes confirmed binding specificity. Binding was observed to blood group B, A and H type 2 epitopes in a strain-dependent manner with slight differences in specificity for A, B or H epitopes allowing RHDV strains to preferentially recognize different subgroups of animals. Strains related to the earliest described RHDV outbreak were not able to bind A, whereas all other genotypes have acquired A binding. In an experimental infection study, rabbits lacking the correct HBGA ligands were resistant to lethal RHDV infection at low challenge doses. Similarly, survivors of outbreaks in wild populations showed increased frequency of weak binding phenotypes, indicating selection for host resistance depending on the strain circulating in the population. HBGAs thus act as attachment factors facilitating infection, while their polymorphism of expression could contribute to generate genetic resistance to RHDV at the population level. Public Library of Science 2011-08-25 /pmc/articles/PMC3161982/ /pubmed/21901093 http://dx.doi.org/10.1371/journal.ppat.1002188 Text en Nyström et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Nyström, Kristina
Le Gall-Reculé, Ghislaine
Grassi, Paola
Abrantes, Joana
Ruvoën-Clouet, Nathalie
Le Moullac-Vaidye, Beatrice
Lopes, Ana M.
Esteves, Pedro J.
Strive, Tanja
Marchandeau, Stéphane
Dell, Anne
Haslam, Stuart M.
Le Pendu, Jacques
Histo-Blood Group Antigens Act as Attachment Factors of Rabbit Hemorrhagic Disease Virus Infection in a Virus Strain-Dependent Manner
title Histo-Blood Group Antigens Act as Attachment Factors of Rabbit Hemorrhagic Disease Virus Infection in a Virus Strain-Dependent Manner
title_full Histo-Blood Group Antigens Act as Attachment Factors of Rabbit Hemorrhagic Disease Virus Infection in a Virus Strain-Dependent Manner
title_fullStr Histo-Blood Group Antigens Act as Attachment Factors of Rabbit Hemorrhagic Disease Virus Infection in a Virus Strain-Dependent Manner
title_full_unstemmed Histo-Blood Group Antigens Act as Attachment Factors of Rabbit Hemorrhagic Disease Virus Infection in a Virus Strain-Dependent Manner
title_short Histo-Blood Group Antigens Act as Attachment Factors of Rabbit Hemorrhagic Disease Virus Infection in a Virus Strain-Dependent Manner
title_sort histo-blood group antigens act as attachment factors of rabbit hemorrhagic disease virus infection in a virus strain-dependent manner
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3161982/
https://www.ncbi.nlm.nih.gov/pubmed/21901093
http://dx.doi.org/10.1371/journal.ppat.1002188
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