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Mediator Subunit 12 Is Required for Neutrophil Development in Zebrafish

Hematopoiesis requires the spatiotemporal organization of regulatory factors to successfully orchestrate diverse lineage specificity from stem and progenitor cells. Med12 is a regulatory component of the large Mediator complex that enables contact between the general RNA polymerase II transcriptiona...

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Autores principales: Keightley, Maria-Cristina, Layton, Judith E., Hayman, John W., Heath, Joan K., Lieschke, Graham J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3162013/
https://www.ncbi.nlm.nih.gov/pubmed/21901140
http://dx.doi.org/10.1371/journal.pone.0023845
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author Keightley, Maria-Cristina
Layton, Judith E.
Hayman, John W.
Heath, Joan K.
Lieschke, Graham J.
author_facet Keightley, Maria-Cristina
Layton, Judith E.
Hayman, John W.
Heath, Joan K.
Lieschke, Graham J.
author_sort Keightley, Maria-Cristina
collection PubMed
description Hematopoiesis requires the spatiotemporal organization of regulatory factors to successfully orchestrate diverse lineage specificity from stem and progenitor cells. Med12 is a regulatory component of the large Mediator complex that enables contact between the general RNA polymerase II transcriptional machinery and enhancer bound regulatory factors. We have identified a new zebrafish med12 allele, syr, with a single missense mutation causing a valine to aspartic acid change at position 1046. Syr shows defects in hematopoiesis, which predominantly affect the myeloid lineage. Syr has identified a hematopoietic cell-specific requirement for Med12, suggesting a new role for this transcriptional regulator.
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spelling pubmed-31620132011-09-07 Mediator Subunit 12 Is Required for Neutrophil Development in Zebrafish Keightley, Maria-Cristina Layton, Judith E. Hayman, John W. Heath, Joan K. Lieschke, Graham J. PLoS One Research Article Hematopoiesis requires the spatiotemporal organization of regulatory factors to successfully orchestrate diverse lineage specificity from stem and progenitor cells. Med12 is a regulatory component of the large Mediator complex that enables contact between the general RNA polymerase II transcriptional machinery and enhancer bound regulatory factors. We have identified a new zebrafish med12 allele, syr, with a single missense mutation causing a valine to aspartic acid change at position 1046. Syr shows defects in hematopoiesis, which predominantly affect the myeloid lineage. Syr has identified a hematopoietic cell-specific requirement for Med12, suggesting a new role for this transcriptional regulator. Public Library of Science 2011-08-25 /pmc/articles/PMC3162013/ /pubmed/21901140 http://dx.doi.org/10.1371/journal.pone.0023845 Text en Keightley et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Keightley, Maria-Cristina
Layton, Judith E.
Hayman, John W.
Heath, Joan K.
Lieschke, Graham J.
Mediator Subunit 12 Is Required for Neutrophil Development in Zebrafish
title Mediator Subunit 12 Is Required for Neutrophil Development in Zebrafish
title_full Mediator Subunit 12 Is Required for Neutrophil Development in Zebrafish
title_fullStr Mediator Subunit 12 Is Required for Neutrophil Development in Zebrafish
title_full_unstemmed Mediator Subunit 12 Is Required for Neutrophil Development in Zebrafish
title_short Mediator Subunit 12 Is Required for Neutrophil Development in Zebrafish
title_sort mediator subunit 12 is required for neutrophil development in zebrafish
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3162013/
https://www.ncbi.nlm.nih.gov/pubmed/21901140
http://dx.doi.org/10.1371/journal.pone.0023845
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