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Mediator Subunit 12 Is Required for Neutrophil Development in Zebrafish
Hematopoiesis requires the spatiotemporal organization of regulatory factors to successfully orchestrate diverse lineage specificity from stem and progenitor cells. Med12 is a regulatory component of the large Mediator complex that enables contact between the general RNA polymerase II transcriptiona...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3162013/ https://www.ncbi.nlm.nih.gov/pubmed/21901140 http://dx.doi.org/10.1371/journal.pone.0023845 |
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author | Keightley, Maria-Cristina Layton, Judith E. Hayman, John W. Heath, Joan K. Lieschke, Graham J. |
author_facet | Keightley, Maria-Cristina Layton, Judith E. Hayman, John W. Heath, Joan K. Lieschke, Graham J. |
author_sort | Keightley, Maria-Cristina |
collection | PubMed |
description | Hematopoiesis requires the spatiotemporal organization of regulatory factors to successfully orchestrate diverse lineage specificity from stem and progenitor cells. Med12 is a regulatory component of the large Mediator complex that enables contact between the general RNA polymerase II transcriptional machinery and enhancer bound regulatory factors. We have identified a new zebrafish med12 allele, syr, with a single missense mutation causing a valine to aspartic acid change at position 1046. Syr shows defects in hematopoiesis, which predominantly affect the myeloid lineage. Syr has identified a hematopoietic cell-specific requirement for Med12, suggesting a new role for this transcriptional regulator. |
format | Online Article Text |
id | pubmed-3162013 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-31620132011-09-07 Mediator Subunit 12 Is Required for Neutrophil Development in Zebrafish Keightley, Maria-Cristina Layton, Judith E. Hayman, John W. Heath, Joan K. Lieschke, Graham J. PLoS One Research Article Hematopoiesis requires the spatiotemporal organization of regulatory factors to successfully orchestrate diverse lineage specificity from stem and progenitor cells. Med12 is a regulatory component of the large Mediator complex that enables contact between the general RNA polymerase II transcriptional machinery and enhancer bound regulatory factors. We have identified a new zebrafish med12 allele, syr, with a single missense mutation causing a valine to aspartic acid change at position 1046. Syr shows defects in hematopoiesis, which predominantly affect the myeloid lineage. Syr has identified a hematopoietic cell-specific requirement for Med12, suggesting a new role for this transcriptional regulator. Public Library of Science 2011-08-25 /pmc/articles/PMC3162013/ /pubmed/21901140 http://dx.doi.org/10.1371/journal.pone.0023845 Text en Keightley et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Keightley, Maria-Cristina Layton, Judith E. Hayman, John W. Heath, Joan K. Lieschke, Graham J. Mediator Subunit 12 Is Required for Neutrophil Development in Zebrafish |
title | Mediator Subunit 12 Is Required for Neutrophil Development in Zebrafish |
title_full | Mediator Subunit 12 Is Required for Neutrophil Development in Zebrafish |
title_fullStr | Mediator Subunit 12 Is Required for Neutrophil Development in Zebrafish |
title_full_unstemmed | Mediator Subunit 12 Is Required for Neutrophil Development in Zebrafish |
title_short | Mediator Subunit 12 Is Required for Neutrophil Development in Zebrafish |
title_sort | mediator subunit 12 is required for neutrophil development in zebrafish |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3162013/ https://www.ncbi.nlm.nih.gov/pubmed/21901140 http://dx.doi.org/10.1371/journal.pone.0023845 |
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