Antibody cross-linking and target elution protocols used for immunoprecipitation significantly modulate signal-to noise ratio in downstream 2D-PAGE analysis

BACKGROUND: Immunoprecipitation and subsequent 2D-PAGE/mass spectrometry are powerful tools to study post-translational protein modifications. Often disregarded in this workflow is the impact of the chemical cross-linker upon antibody affinity, as well as incomplete elution of primary target protein...

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Detalles Bibliográficos
Autores principales: Sousa, Mirta ML, Steen, Kristian W, Hagen, Lars, Slupphaug, Geir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3162493/
https://www.ncbi.nlm.nih.gov/pubmed/21816076
http://dx.doi.org/10.1186/1477-5956-9-45

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