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Regulation of cyclin-dependent kinase 4 translation through CUG-binding protein 1 and microRNA-222 by polyamines

The amino acid–derived polyamines are organic cations that are essential for growth in all mammalian cells, but their exact roles at the molecular level remain largely unknown. Here we provide evidence that polyamines promote the translation of cyclin-dependent kinase 4 (CDK4) by the action of CUG-b...

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Autores principales: Xiao, Lan, Cui, Yu-Hong, Rao, Jaladanki N., Zou, Tongtong, Liu, Lan, Smith, Alexis, Turner, Douglas J., Gorospe, Myriam, Wang, Jian-Ying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3164454/
https://www.ncbi.nlm.nih.gov/pubmed/21737690
http://dx.doi.org/10.1091/mbc.E11-01-0069
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author Xiao, Lan
Cui, Yu-Hong
Rao, Jaladanki N.
Zou, Tongtong
Liu, Lan
Smith, Alexis
Turner, Douglas J.
Gorospe, Myriam
Wang, Jian-Ying
author_facet Xiao, Lan
Cui, Yu-Hong
Rao, Jaladanki N.
Zou, Tongtong
Liu, Lan
Smith, Alexis
Turner, Douglas J.
Gorospe, Myriam
Wang, Jian-Ying
author_sort Xiao, Lan
collection PubMed
description The amino acid–derived polyamines are organic cations that are essential for growth in all mammalian cells, but their exact roles at the molecular level remain largely unknown. Here we provide evidence that polyamines promote the translation of cyclin-dependent kinase 4 (CDK4) by the action of CUG-binding protein 1 (CUGBP1) and microRNA-222 (miR-222) in intestinal epithelial cells. Both CUGBP1 and miR-222 were found to bind the CDK4 mRNA coding region and 3′-untranslated region and repressed CDK4 translation synergistically. Depletion of cellular polyamines increased cytoplasmic CUGBP1 abundance and miR-222 levels, induced their associations with the CDK4 mRNA, and inhibited CDK4 translation, whereas increasing the levels of cellular polyamines decreased CDK4 mRNA interaction with CUGBP1 and miR-222, in turn inducing CDK4 expression. Polyamine-deficient cells exhibited an increased colocalization of tagged CDK4 mRNA with processing bodies; this colocalization was abolished by silencing CUGBP1 and miR-222. Together, our findings indicate that polyamine-regulated CUGBP1 and miR-222 modulate CDK4 translation at least in part by altering the recruitment of CDK4 mRNA to processing bodies.
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spelling pubmed-31644542011-11-16 Regulation of cyclin-dependent kinase 4 translation through CUG-binding protein 1 and microRNA-222 by polyamines Xiao, Lan Cui, Yu-Hong Rao, Jaladanki N. Zou, Tongtong Liu, Lan Smith, Alexis Turner, Douglas J. Gorospe, Myriam Wang, Jian-Ying Mol Biol Cell Articles The amino acid–derived polyamines are organic cations that are essential for growth in all mammalian cells, but their exact roles at the molecular level remain largely unknown. Here we provide evidence that polyamines promote the translation of cyclin-dependent kinase 4 (CDK4) by the action of CUG-binding protein 1 (CUGBP1) and microRNA-222 (miR-222) in intestinal epithelial cells. Both CUGBP1 and miR-222 were found to bind the CDK4 mRNA coding region and 3′-untranslated region and repressed CDK4 translation synergistically. Depletion of cellular polyamines increased cytoplasmic CUGBP1 abundance and miR-222 levels, induced their associations with the CDK4 mRNA, and inhibited CDK4 translation, whereas increasing the levels of cellular polyamines decreased CDK4 mRNA interaction with CUGBP1 and miR-222, in turn inducing CDK4 expression. Polyamine-deficient cells exhibited an increased colocalization of tagged CDK4 mRNA with processing bodies; this colocalization was abolished by silencing CUGBP1 and miR-222. Together, our findings indicate that polyamine-regulated CUGBP1 and miR-222 modulate CDK4 translation at least in part by altering the recruitment of CDK4 mRNA to processing bodies. The American Society for Cell Biology 2011-09-01 /pmc/articles/PMC3164454/ /pubmed/21737690 http://dx.doi.org/10.1091/mbc.E11-01-0069 Text en © 2011 Xiao et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Xiao, Lan
Cui, Yu-Hong
Rao, Jaladanki N.
Zou, Tongtong
Liu, Lan
Smith, Alexis
Turner, Douglas J.
Gorospe, Myriam
Wang, Jian-Ying
Regulation of cyclin-dependent kinase 4 translation through CUG-binding protein 1 and microRNA-222 by polyamines
title Regulation of cyclin-dependent kinase 4 translation through CUG-binding protein 1 and microRNA-222 by polyamines
title_full Regulation of cyclin-dependent kinase 4 translation through CUG-binding protein 1 and microRNA-222 by polyamines
title_fullStr Regulation of cyclin-dependent kinase 4 translation through CUG-binding protein 1 and microRNA-222 by polyamines
title_full_unstemmed Regulation of cyclin-dependent kinase 4 translation through CUG-binding protein 1 and microRNA-222 by polyamines
title_short Regulation of cyclin-dependent kinase 4 translation through CUG-binding protein 1 and microRNA-222 by polyamines
title_sort regulation of cyclin-dependent kinase 4 translation through cug-binding protein 1 and microrna-222 by polyamines
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3164454/
https://www.ncbi.nlm.nih.gov/pubmed/21737690
http://dx.doi.org/10.1091/mbc.E11-01-0069
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