Cargando…

Fine-tuning of the Msn2/4–mediated yeast stress responses as revealed by systematic deletion of Msn2/4 partners

The Msn2 and Msn4 transcription factors play major roles in the yeast general stress response by mediating the transcription of hundreds of genes. Despite extensive information on Msn2/4–mediated gene expression profiles, much less is known regarding the network of proteins that regulate its activit...

Descripción completa

Detalles Bibliográficos
Autores principales: Sadeh, Amit, Movshovich, Natalia, Volokh, Misha, Gheber, Larisa, Aharoni, Amir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3164460/
https://www.ncbi.nlm.nih.gov/pubmed/21757539
http://dx.doi.org/10.1091/mbc.E10-12-1007
_version_ 1782211042329231360
author Sadeh, Amit
Movshovich, Natalia
Volokh, Misha
Gheber, Larisa
Aharoni, Amir
author_facet Sadeh, Amit
Movshovich, Natalia
Volokh, Misha
Gheber, Larisa
Aharoni, Amir
author_sort Sadeh, Amit
collection PubMed
description The Msn2 and Msn4 transcription factors play major roles in the yeast general stress response by mediating the transcription of hundreds of genes. Despite extensive information on Msn2/4–mediated gene expression profiles, much less is known regarding the network of proteins that regulate its activity. Here we describe a systematic approach designed to examine the roles of 35 Msn2/4 partners in regulating Msn2/4 transcriptional activity in the face of four different environmental conditions. Our analysis indicates that single deletions of 26 Msn2/4 partners significantly affect Msn2/4 transcription activity under four different conditions. The low functional redundancy of the Msn2 regulatory network indicates that Msn2/4 activity is finely tuned by many of Msn2/4 partners to provide an optimized stress response through differential activation, nuclear localization, degradation, and chromatin remodeling. Our specific analysis of Msn2 activity showed that a relatively large number of partners act to suppress Msn2 activity under nonstress conditions through independent mechanisms, including cytoplasmic retention, proteosome-mediated Msn2 degradation, and chromatin remodeling. Such negative regulation is crucial to minimize the cost of uncontrolled stress response gene expression and ensures a high growth rate in the absence of stress.
format Online
Article
Text
id pubmed-3164460
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher The American Society for Cell Biology
record_format MEDLINE/PubMed
spelling pubmed-31644602011-11-16 Fine-tuning of the Msn2/4–mediated yeast stress responses as revealed by systematic deletion of Msn2/4 partners Sadeh, Amit Movshovich, Natalia Volokh, Misha Gheber, Larisa Aharoni, Amir Mol Biol Cell Articles The Msn2 and Msn4 transcription factors play major roles in the yeast general stress response by mediating the transcription of hundreds of genes. Despite extensive information on Msn2/4–mediated gene expression profiles, much less is known regarding the network of proteins that regulate its activity. Here we describe a systematic approach designed to examine the roles of 35 Msn2/4 partners in regulating Msn2/4 transcriptional activity in the face of four different environmental conditions. Our analysis indicates that single deletions of 26 Msn2/4 partners significantly affect Msn2/4 transcription activity under four different conditions. The low functional redundancy of the Msn2 regulatory network indicates that Msn2/4 activity is finely tuned by many of Msn2/4 partners to provide an optimized stress response through differential activation, nuclear localization, degradation, and chromatin remodeling. Our specific analysis of Msn2 activity showed that a relatively large number of partners act to suppress Msn2 activity under nonstress conditions through independent mechanisms, including cytoplasmic retention, proteosome-mediated Msn2 degradation, and chromatin remodeling. Such negative regulation is crucial to minimize the cost of uncontrolled stress response gene expression and ensures a high growth rate in the absence of stress. The American Society for Cell Biology 2011-09-01 /pmc/articles/PMC3164460/ /pubmed/21757539 http://dx.doi.org/10.1091/mbc.E10-12-1007 Text en © 2011 Sadeh et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Sadeh, Amit
Movshovich, Natalia
Volokh, Misha
Gheber, Larisa
Aharoni, Amir
Fine-tuning of the Msn2/4–mediated yeast stress responses as revealed by systematic deletion of Msn2/4 partners
title Fine-tuning of the Msn2/4–mediated yeast stress responses as revealed by systematic deletion of Msn2/4 partners
title_full Fine-tuning of the Msn2/4–mediated yeast stress responses as revealed by systematic deletion of Msn2/4 partners
title_fullStr Fine-tuning of the Msn2/4–mediated yeast stress responses as revealed by systematic deletion of Msn2/4 partners
title_full_unstemmed Fine-tuning of the Msn2/4–mediated yeast stress responses as revealed by systematic deletion of Msn2/4 partners
title_short Fine-tuning of the Msn2/4–mediated yeast stress responses as revealed by systematic deletion of Msn2/4 partners
title_sort fine-tuning of the msn2/4–mediated yeast stress responses as revealed by systematic deletion of msn2/4 partners
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3164460/
https://www.ncbi.nlm.nih.gov/pubmed/21757539
http://dx.doi.org/10.1091/mbc.E10-12-1007
work_keys_str_mv AT sadehamit finetuningofthemsn24mediatedyeaststressresponsesasrevealedbysystematicdeletionofmsn24partners
AT movshovichnatalia finetuningofthemsn24mediatedyeaststressresponsesasrevealedbysystematicdeletionofmsn24partners
AT volokhmisha finetuningofthemsn24mediatedyeaststressresponsesasrevealedbysystematicdeletionofmsn24partners
AT gheberlarisa finetuningofthemsn24mediatedyeaststressresponsesasrevealedbysystematicdeletionofmsn24partners
AT aharoniamir finetuningofthemsn24mediatedyeaststressresponsesasrevealedbysystematicdeletionofmsn24partners