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Anillin promotes astral microtubule-directed cortical myosin polarization

Assembly of a cytokinetic contractile ring is a form of cell polarization in which the equatorial cell cortex becomes differentiated from the polar regions. Microtubules direct cytokinetic polarization via the central spindle and astral microtubules. The mechanism of central spindle–directed furrow...

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Autores principales: Tse, Yu Chung, Piekny, Alisa, Glotzer, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3164463/
https://www.ncbi.nlm.nih.gov/pubmed/21737681
http://dx.doi.org/10.1091/mbc.E11-05-0399
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author Tse, Yu Chung
Piekny, Alisa
Glotzer, Michael
author_facet Tse, Yu Chung
Piekny, Alisa
Glotzer, Michael
author_sort Tse, Yu Chung
collection PubMed
description Assembly of a cytokinetic contractile ring is a form of cell polarization in which the equatorial cell cortex becomes differentiated from the polar regions. Microtubules direct cytokinetic polarization via the central spindle and astral microtubules. The mechanism of central spindle–directed furrow formation is reasonably well understood, but the aster-directed pathway is not. In aster-directed furrowing, cytoskeletal factors accumulate to high levels at sites distal to the asters and at reduced levels at cortical sites near the asters. In this paper, we demonstrate that the cytoskeletal organizing protein anillin (ANI-1) promotes the formation of an aster-directed furrow in Caenorhabditis elegans embryos. Microtubule-directed nonmuscle myosin II polarization is aberrant in embryos depleted of ANI-1. In contrast, microtubule-directed polarized ANI-1 localization is largely unaffected by myosin II depletion. Consistent with a role in the induction of cortical asymmetry, ANI-1 also contributes to the polarization of arrested oocytes. Anillin has an evolutionarily conserved capacity to associate with microtubules, possibly providing an inhibitory mechanism to promote polarization of the cell cortex.
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spelling pubmed-31644632011-11-16 Anillin promotes astral microtubule-directed cortical myosin polarization Tse, Yu Chung Piekny, Alisa Glotzer, Michael Mol Biol Cell Articles Assembly of a cytokinetic contractile ring is a form of cell polarization in which the equatorial cell cortex becomes differentiated from the polar regions. Microtubules direct cytokinetic polarization via the central spindle and astral microtubules. The mechanism of central spindle–directed furrow formation is reasonably well understood, but the aster-directed pathway is not. In aster-directed furrowing, cytoskeletal factors accumulate to high levels at sites distal to the asters and at reduced levels at cortical sites near the asters. In this paper, we demonstrate that the cytoskeletal organizing protein anillin (ANI-1) promotes the formation of an aster-directed furrow in Caenorhabditis elegans embryos. Microtubule-directed nonmuscle myosin II polarization is aberrant in embryos depleted of ANI-1. In contrast, microtubule-directed polarized ANI-1 localization is largely unaffected by myosin II depletion. Consistent with a role in the induction of cortical asymmetry, ANI-1 also contributes to the polarization of arrested oocytes. Anillin has an evolutionarily conserved capacity to associate with microtubules, possibly providing an inhibitory mechanism to promote polarization of the cell cortex. The American Society for Cell Biology 2011-09-01 /pmc/articles/PMC3164463/ /pubmed/21737681 http://dx.doi.org/10.1091/mbc.E11-05-0399 Text en © 2011 Tse et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Tse, Yu Chung
Piekny, Alisa
Glotzer, Michael
Anillin promotes astral microtubule-directed cortical myosin polarization
title Anillin promotes astral microtubule-directed cortical myosin polarization
title_full Anillin promotes astral microtubule-directed cortical myosin polarization
title_fullStr Anillin promotes astral microtubule-directed cortical myosin polarization
title_full_unstemmed Anillin promotes astral microtubule-directed cortical myosin polarization
title_short Anillin promotes astral microtubule-directed cortical myosin polarization
title_sort anillin promotes astral microtubule-directed cortical myosin polarization
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3164463/
https://www.ncbi.nlm.nih.gov/pubmed/21737681
http://dx.doi.org/10.1091/mbc.E11-05-0399
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