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Effect of estradiol on proliferation and differentiation of side population stem/progenitor cells from murine endometrium

BACKGROUND: In our previous study, endometrium side population cells (SP cells) were isolated from postpartum murine uterus, and characterized by a heterogeneous population of stem/progenitor cells. In this study, we investigated the effect of estrogen on the proliferation and differentiation of SP...

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Autores principales: Xu, Jing, Hu, Fei-Fei, Cui, Yu-Gui, Luo, Jian, Jiang, Chun-Yan, Gao, Li, Qian, Xiao-Qiao, Mao, Yun-Dong, Liu, Jia-Yin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3164598/
https://www.ncbi.nlm.nih.gov/pubmed/21801373
http://dx.doi.org/10.1186/1477-7827-9-103
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author Xu, Jing
Hu, Fei-Fei
Cui, Yu-Gui
Luo, Jian
Jiang, Chun-Yan
Gao, Li
Qian, Xiao-Qiao
Mao, Yun-Dong
Liu, Jia-Yin
author_facet Xu, Jing
Hu, Fei-Fei
Cui, Yu-Gui
Luo, Jian
Jiang, Chun-Yan
Gao, Li
Qian, Xiao-Qiao
Mao, Yun-Dong
Liu, Jia-Yin
author_sort Xu, Jing
collection PubMed
description BACKGROUND: In our previous study, endometrium side population cells (SP cells) were isolated from postpartum murine uterus, and characterized by a heterogeneous population of stem/progenitor cells. In this study, we investigated the effect of estrogen on the proliferation and differentiation of SP cells. METHODS: SP and non-SP cells of postpartum murine endometrium were isolated by DNA dye Hoechst 33342. The expression of estrogen receptor 1 (ESR1) was measured by reverse transcription polymerase chain reaction (RT-PCR), Real-time PCR, Western blot, immunofluorescence and immunohistochemistry. The proliferation and differentiation of SP cells treated with different concentrations [10(-8) M-10(-6) M] of estradiol (E2) and E2+ ICI182780 (Faslodex, inhibitor of ESR1) were measured by 3-(4, 5-dimethylthiazoly1-2)-2,5-diphenyltetrazolium bromide(MTT) and clonogenic assays. RESULTS: (1) SP cells expressed ESR1 at a higher level than non-SP cells. (2) The level of E2 in the serum and the expression of ESR1 in the uterus of postpartum murine changed in the same manner with the ratio of SP cells to total uterus cells at a different postpartum time point. ESR1, as ABCG2 is also predominantly located in the stroma and the glandular epithelium of the uterus. (3) 10(-6) M E2 notably promoted the proliferation of SP cells after treatment for 24 h. This effect could be inhibited by ICI182780. E2 at the concentration of 10(-7) M or 10(-8) M was sent to impair the large cloning efficiency (CE) of SP cells. CONCLUSIONS: The effect of estrogen on the proliferation and differentiation of endometrium SP cells via ESR1 was observed and it was in a concentration dependent fashion. Clearly, more work is needed to understand the in vivo effect of E2 at the physiological concentration on the differentiation of SP cells.
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spelling pubmed-31645982011-09-02 Effect of estradiol on proliferation and differentiation of side population stem/progenitor cells from murine endometrium Xu, Jing Hu, Fei-Fei Cui, Yu-Gui Luo, Jian Jiang, Chun-Yan Gao, Li Qian, Xiao-Qiao Mao, Yun-Dong Liu, Jia-Yin Reprod Biol Endocrinol Research BACKGROUND: In our previous study, endometrium side population cells (SP cells) were isolated from postpartum murine uterus, and characterized by a heterogeneous population of stem/progenitor cells. In this study, we investigated the effect of estrogen on the proliferation and differentiation of SP cells. METHODS: SP and non-SP cells of postpartum murine endometrium were isolated by DNA dye Hoechst 33342. The expression of estrogen receptor 1 (ESR1) was measured by reverse transcription polymerase chain reaction (RT-PCR), Real-time PCR, Western blot, immunofluorescence and immunohistochemistry. The proliferation and differentiation of SP cells treated with different concentrations [10(-8) M-10(-6) M] of estradiol (E2) and E2+ ICI182780 (Faslodex, inhibitor of ESR1) were measured by 3-(4, 5-dimethylthiazoly1-2)-2,5-diphenyltetrazolium bromide(MTT) and clonogenic assays. RESULTS: (1) SP cells expressed ESR1 at a higher level than non-SP cells. (2) The level of E2 in the serum and the expression of ESR1 in the uterus of postpartum murine changed in the same manner with the ratio of SP cells to total uterus cells at a different postpartum time point. ESR1, as ABCG2 is also predominantly located in the stroma and the glandular epithelium of the uterus. (3) 10(-6) M E2 notably promoted the proliferation of SP cells after treatment for 24 h. This effect could be inhibited by ICI182780. E2 at the concentration of 10(-7) M or 10(-8) M was sent to impair the large cloning efficiency (CE) of SP cells. CONCLUSIONS: The effect of estrogen on the proliferation and differentiation of endometrium SP cells via ESR1 was observed and it was in a concentration dependent fashion. Clearly, more work is needed to understand the in vivo effect of E2 at the physiological concentration on the differentiation of SP cells. BioMed Central 2011-07-29 /pmc/articles/PMC3164598/ /pubmed/21801373 http://dx.doi.org/10.1186/1477-7827-9-103 Text en Copyright ©2011 Xu et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Xu, Jing
Hu, Fei-Fei
Cui, Yu-Gui
Luo, Jian
Jiang, Chun-Yan
Gao, Li
Qian, Xiao-Qiao
Mao, Yun-Dong
Liu, Jia-Yin
Effect of estradiol on proliferation and differentiation of side population stem/progenitor cells from murine endometrium
title Effect of estradiol on proliferation and differentiation of side population stem/progenitor cells from murine endometrium
title_full Effect of estradiol on proliferation and differentiation of side population stem/progenitor cells from murine endometrium
title_fullStr Effect of estradiol on proliferation and differentiation of side population stem/progenitor cells from murine endometrium
title_full_unstemmed Effect of estradiol on proliferation and differentiation of side population stem/progenitor cells from murine endometrium
title_short Effect of estradiol on proliferation and differentiation of side population stem/progenitor cells from murine endometrium
title_sort effect of estradiol on proliferation and differentiation of side population stem/progenitor cells from murine endometrium
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3164598/
https://www.ncbi.nlm.nih.gov/pubmed/21801373
http://dx.doi.org/10.1186/1477-7827-9-103
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