Revealing Off-Target Cleavage Specificities of Zinc Finger Nucleases by In Vitro Selection

Engineered zinc finger nucleases (ZFNs) are promising tools for genome manipulation and determining off-target cleavage sites of these enzymes is of great interest. We developed an in vitro selection method that interrogates 10(11) DNA sequences for cleavage by active, dimeric ZFNs. The method revea...

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Detalles Bibliográficos
Autores principales: Pattanayak, Vikram, Ramirez, Cherie L., Joung, J. Keith, Liu, David R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3164905/
https://www.ncbi.nlm.nih.gov/pubmed/21822273
http://dx.doi.org/10.1038/nmeth.1670

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3164905/
https://www.ncbi.nlm.nih.gov/pubmed/21822273
http://dx.doi.org/10.1038/nmeth.1670

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