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Cytotoxicity of gamma-ray in rat immature hippocampal neurons
This in vitro study evaluated the detrimental effect of acute gamma (γ)-irradiation on rat immature hippocampal neurons. Rat immature hippocampal neurons (0.5 day in vitro) were irradiated with 0~4 Gy γ-rays. Cytotoxicity was analyzed using a lactate dehydrogenase release assay at 24 h after γ-irrad...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Society of Veterinary Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3165147/ https://www.ncbi.nlm.nih.gov/pubmed/21897091 http://dx.doi.org/10.4142/jvs.2011.12.3.203 |
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author | Yang, Miyoung Song, Myoung-Sub Kim, Sung-Ho Kim, Jong-Choon Kim, Joong-Sun Shin, Taekyun Moon, Changjong |
author_facet | Yang, Miyoung Song, Myoung-Sub Kim, Sung-Ho Kim, Jong-Choon Kim, Joong-Sun Shin, Taekyun Moon, Changjong |
author_sort | Yang, Miyoung |
collection | PubMed |
description | This in vitro study evaluated the detrimental effect of acute gamma (γ)-irradiation on rat immature hippocampal neurons. Rat immature hippocampal neurons (0.5 day in vitro) were irradiated with 0~4 Gy γ-rays. Cytotoxicity was analyzed using a lactate dehydrogenase release assay at 24 h after γ-irradiation. Radiation-induced cytotoxicity in immature hippocampal neurons increased in a dose-dependent manner. Pre-treatments of pro-apoptotic caspase inhibitors and anti-oxidative substances significantly blocked γ-irradiation-induced cytotoxicity in immature hippocampal neurons. The results suggest that the caspase-dependent cytotoxicity of γ-rays in immature hippocampal cultured neurons may be caused by oxidative stress. |
format | Online Article Text |
id | pubmed-3165147 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | The Korean Society of Veterinary Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-31651472011-09-12 Cytotoxicity of gamma-ray in rat immature hippocampal neurons Yang, Miyoung Song, Myoung-Sub Kim, Sung-Ho Kim, Jong-Choon Kim, Joong-Sun Shin, Taekyun Moon, Changjong J Vet Sci Original Article This in vitro study evaluated the detrimental effect of acute gamma (γ)-irradiation on rat immature hippocampal neurons. Rat immature hippocampal neurons (0.5 day in vitro) were irradiated with 0~4 Gy γ-rays. Cytotoxicity was analyzed using a lactate dehydrogenase release assay at 24 h after γ-irradiation. Radiation-induced cytotoxicity in immature hippocampal neurons increased in a dose-dependent manner. Pre-treatments of pro-apoptotic caspase inhibitors and anti-oxidative substances significantly blocked γ-irradiation-induced cytotoxicity in immature hippocampal neurons. The results suggest that the caspase-dependent cytotoxicity of γ-rays in immature hippocampal cultured neurons may be caused by oxidative stress. The Korean Society of Veterinary Science 2011-09 2011-08-23 /pmc/articles/PMC3165147/ /pubmed/21897091 http://dx.doi.org/10.4142/jvs.2011.12.3.203 Text en Copyright © 2011 The Korean Society of Veterinary Science https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Yang, Miyoung Song, Myoung-Sub Kim, Sung-Ho Kim, Jong-Choon Kim, Joong-Sun Shin, Taekyun Moon, Changjong Cytotoxicity of gamma-ray in rat immature hippocampal neurons |
title | Cytotoxicity of gamma-ray in rat immature hippocampal neurons |
title_full | Cytotoxicity of gamma-ray in rat immature hippocampal neurons |
title_fullStr | Cytotoxicity of gamma-ray in rat immature hippocampal neurons |
title_full_unstemmed | Cytotoxicity of gamma-ray in rat immature hippocampal neurons |
title_short | Cytotoxicity of gamma-ray in rat immature hippocampal neurons |
title_sort | cytotoxicity of gamma-ray in rat immature hippocampal neurons |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3165147/ https://www.ncbi.nlm.nih.gov/pubmed/21897091 http://dx.doi.org/10.4142/jvs.2011.12.3.203 |
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