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The Source of Spontaneous Activity in the Main Olfactory Bulb of the Rat

INTRODUCTION: In vivo, most neurons in the main olfactory bulb exhibit robust spontaneous activity. This paper tests the hypothesis that spontaneous activity in olfactory receptor neurons drives much of the spontaneous activity in mitral and tufted cells via excitatory synapses. METHODS: Single unit...

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Autores principales: Stakic, Josif, Suchanek, Jessica M., Ziegler, Geoffrey P., Griff, Edwin R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3166066/
https://www.ncbi.nlm.nih.gov/pubmed/21912614
http://dx.doi.org/10.1371/journal.pone.0023990
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author Stakic, Josif
Suchanek, Jessica M.
Ziegler, Geoffrey P.
Griff, Edwin R.
author_facet Stakic, Josif
Suchanek, Jessica M.
Ziegler, Geoffrey P.
Griff, Edwin R.
author_sort Stakic, Josif
collection PubMed
description INTRODUCTION: In vivo, most neurons in the main olfactory bulb exhibit robust spontaneous activity. This paper tests the hypothesis that spontaneous activity in olfactory receptor neurons drives much of the spontaneous activity in mitral and tufted cells via excitatory synapses. METHODS: Single units were recorded in vivo from the main olfactory bulb of a rat before, during, and after application of lidocaine to the olfactory nerve. The effect of lidocaine on the conduction of action potentials from the olfactory epithelium to the olfactory bulb was assessed by electrically stimulating the olfactory nerve rostral to the application site and monitoring the field potential evoked in the bulb. RESULTS: Lidocaine caused a significant decrease in the amplitude of the olfactory nerve evoked field potential that was recorded in the olfactory bulb. By contrast, the lidocaine block did not significantly alter the spontaneous activity of single units in the bulb, nor did it alter the field potential evoked by electrical stimulation of the lateral olfactory tract. Lidocaine block also did not change the temporal patters of action potential or their synchronization with respiration. CONCLUSIONS: Spontaneous activity in neurons of the main olfactory bulb is not driven mainly by activity in olfactory receptor neurons despite the extensive convergence onto mitral and tufted cells. These results suggest that spontaneous activity of mitral and tufted is either an inherent property of these cells or is driven by centrifugal inputs to the bulb.
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spelling pubmed-31660662011-09-12 The Source of Spontaneous Activity in the Main Olfactory Bulb of the Rat Stakic, Josif Suchanek, Jessica M. Ziegler, Geoffrey P. Griff, Edwin R. PLoS One Research Article INTRODUCTION: In vivo, most neurons in the main olfactory bulb exhibit robust spontaneous activity. This paper tests the hypothesis that spontaneous activity in olfactory receptor neurons drives much of the spontaneous activity in mitral and tufted cells via excitatory synapses. METHODS: Single units were recorded in vivo from the main olfactory bulb of a rat before, during, and after application of lidocaine to the olfactory nerve. The effect of lidocaine on the conduction of action potentials from the olfactory epithelium to the olfactory bulb was assessed by electrically stimulating the olfactory nerve rostral to the application site and monitoring the field potential evoked in the bulb. RESULTS: Lidocaine caused a significant decrease in the amplitude of the olfactory nerve evoked field potential that was recorded in the olfactory bulb. By contrast, the lidocaine block did not significantly alter the spontaneous activity of single units in the bulb, nor did it alter the field potential evoked by electrical stimulation of the lateral olfactory tract. Lidocaine block also did not change the temporal patters of action potential or their synchronization with respiration. CONCLUSIONS: Spontaneous activity in neurons of the main olfactory bulb is not driven mainly by activity in olfactory receptor neurons despite the extensive convergence onto mitral and tufted cells. These results suggest that spontaneous activity of mitral and tufted is either an inherent property of these cells or is driven by centrifugal inputs to the bulb. Public Library of Science 2011-08-30 /pmc/articles/PMC3166066/ /pubmed/21912614 http://dx.doi.org/10.1371/journal.pone.0023990 Text en Stakic et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Stakic, Josif
Suchanek, Jessica M.
Ziegler, Geoffrey P.
Griff, Edwin R.
The Source of Spontaneous Activity in the Main Olfactory Bulb of the Rat
title The Source of Spontaneous Activity in the Main Olfactory Bulb of the Rat
title_full The Source of Spontaneous Activity in the Main Olfactory Bulb of the Rat
title_fullStr The Source of Spontaneous Activity in the Main Olfactory Bulb of the Rat
title_full_unstemmed The Source of Spontaneous Activity in the Main Olfactory Bulb of the Rat
title_short The Source of Spontaneous Activity in the Main Olfactory Bulb of the Rat
title_sort source of spontaneous activity in the main olfactory bulb of the rat
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3166066/
https://www.ncbi.nlm.nih.gov/pubmed/21912614
http://dx.doi.org/10.1371/journal.pone.0023990
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