Macromolecular Fingerprinting of Sulfolobus Species in Biofilm: A Transcriptomic and Proteomic Approach Combined with Spectroscopic Analysis

[Image: see text] Microorganisms in nature often live in surface-associated sessile communities, encased in a self-produced matrix, referred to as biofilms. Biofilms have been well studied in bacteria but in a limited way for archaea. We have recently characterized biofilm formation in three closely...

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Autores principales: Koerdt, Andrea, Orell, Alvaro, Pham, Trong Khoa, Mukherjee, Joy, Wlodkowski, Alexander, Karunakaran, Esther, Biggs, Catherine A., Wright, Phillip C., Albers, Sonja-Verena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2011
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3166137/
https://www.ncbi.nlm.nih.gov/pubmed/21761944
http://dx.doi.org/10.1021/pr2003006
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author Koerdt, Andrea
Orell, Alvaro
Pham, Trong Khoa
Mukherjee, Joy
Wlodkowski, Alexander
Karunakaran, Esther
Biggs, Catherine A.
Wright, Phillip C.
Albers, Sonja-Verena
author_facet Koerdt, Andrea
Orell, Alvaro
Pham, Trong Khoa
Mukherjee, Joy
Wlodkowski, Alexander
Karunakaran, Esther
Biggs, Catherine A.
Wright, Phillip C.
Albers, Sonja-Verena
author_sort Koerdt, Andrea
collection PubMed
description [Image: see text] Microorganisms in nature often live in surface-associated sessile communities, encased in a self-produced matrix, referred to as biofilms. Biofilms have been well studied in bacteria but in a limited way for archaea. We have recently characterized biofilm formation in three closely related hyperthermophilic crenarchaeotes: Sulfolobus acidocaldarius, S. solfataricus, and S. tokodaii. These strains form different communities ranging from simple carpet structures in S. solfataricus to high density tower-like structures in S. acidocaldarius under static condition. Here, we combine spectroscopic, proteomic, and transcriptomic analyses to describe physiological and regulatory features associated with biofilms. Spectroscopic analysis reveals that in comparison to planktonic life-style, biofilm life-style has distinctive influence on the physiology of each Sulfolobus spp. Proteomic and transcriptomic data show that biofilm-forming life-style is strain specific (eg ca. 15% of the S. acidocaldarius genes were differently expressed, S. solfataricus and S. tokodaii had ∼3.4 and ∼1%, respectively). The -omic data showed that regulated ORFs were widely distributed in basic cellular functions, including surface modifications. Several regulated genes are common to biofilm-forming cells in all three species. One of the most striking common response genes include putative Lrs14-like transcriptional regulators, indicating their possible roles as a key regulatory factor in biofilm development.
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spelling pubmed-31661372011-09-06 Macromolecular Fingerprinting of Sulfolobus Species in Biofilm: A Transcriptomic and Proteomic Approach Combined with Spectroscopic Analysis Koerdt, Andrea Orell, Alvaro Pham, Trong Khoa Mukherjee, Joy Wlodkowski, Alexander Karunakaran, Esther Biggs, Catherine A. Wright, Phillip C. Albers, Sonja-Verena J Proteome Res [Image: see text] Microorganisms in nature often live in surface-associated sessile communities, encased in a self-produced matrix, referred to as biofilms. Biofilms have been well studied in bacteria but in a limited way for archaea. We have recently characterized biofilm formation in three closely related hyperthermophilic crenarchaeotes: Sulfolobus acidocaldarius, S. solfataricus, and S. tokodaii. These strains form different communities ranging from simple carpet structures in S. solfataricus to high density tower-like structures in S. acidocaldarius under static condition. Here, we combine spectroscopic, proteomic, and transcriptomic analyses to describe physiological and regulatory features associated with biofilms. Spectroscopic analysis reveals that in comparison to planktonic life-style, biofilm life-style has distinctive influence on the physiology of each Sulfolobus spp. Proteomic and transcriptomic data show that biofilm-forming life-style is strain specific (eg ca. 15% of the S. acidocaldarius genes were differently expressed, S. solfataricus and S. tokodaii had ∼3.4 and ∼1%, respectively). The -omic data showed that regulated ORFs were widely distributed in basic cellular functions, including surface modifications. Several regulated genes are common to biofilm-forming cells in all three species. One of the most striking common response genes include putative Lrs14-like transcriptional regulators, indicating their possible roles as a key regulatory factor in biofilm development. American Chemical Society 2011-07-18 2011-09-02 /pmc/articles/PMC3166137/ /pubmed/21761944 http://dx.doi.org/10.1021/pr2003006 Text en Copyright © 2011 American Chemical Society http://pubs.acs.org This is an open-access article distributed under the ACS AuthorChoice Terms & Conditions. Any use of this article, must conform to the terms of that license which are available at http://pubs.acs.org.
spellingShingle Koerdt, Andrea
Orell, Alvaro
Pham, Trong Khoa
Mukherjee, Joy
Wlodkowski, Alexander
Karunakaran, Esther
Biggs, Catherine A.
Wright, Phillip C.
Albers, Sonja-Verena
Macromolecular Fingerprinting of Sulfolobus Species in Biofilm: A Transcriptomic and Proteomic Approach Combined with Spectroscopic Analysis
title Macromolecular Fingerprinting of Sulfolobus Species in Biofilm: A Transcriptomic and Proteomic Approach Combined with Spectroscopic Analysis
title_full Macromolecular Fingerprinting of Sulfolobus Species in Biofilm: A Transcriptomic and Proteomic Approach Combined with Spectroscopic Analysis
title_fullStr Macromolecular Fingerprinting of Sulfolobus Species in Biofilm: A Transcriptomic and Proteomic Approach Combined with Spectroscopic Analysis
title_full_unstemmed Macromolecular Fingerprinting of Sulfolobus Species in Biofilm: A Transcriptomic and Proteomic Approach Combined with Spectroscopic Analysis
title_short Macromolecular Fingerprinting of Sulfolobus Species in Biofilm: A Transcriptomic and Proteomic Approach Combined with Spectroscopic Analysis
title_sort macromolecular fingerprinting of sulfolobus species in biofilm: a transcriptomic and proteomic approach combined with spectroscopic analysis
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3166137/
https://www.ncbi.nlm.nih.gov/pubmed/21761944
http://dx.doi.org/10.1021/pr2003006
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