Cargando…

Evidence for Time-of-Day Dependent Effect of Neurotoxic Dorsomedial Hypothalamic Lesions on Food Anticipatory Circadian Rhythms in Rats

The dorsomedial hypothalamus (DMH) is a site of circadian clock gene and immediate early gene expression inducible by daytime restricted feeding schedules that entrain food anticipatory circadian rhythms in rats and mice. The role of the DMH in the expression of anticipatory rhythms has been evaluat...

Descripción completa

Detalles Bibliográficos
Autores principales: Landry, Glenn J., Kent, Brianne A., Patton, Danica F., Jaholkowski, Mark, Marchant, Elliott G., Mistlberger, Ralph E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3166290/
https://www.ncbi.nlm.nih.gov/pubmed/21912674
http://dx.doi.org/10.1371/journal.pone.0024187
_version_ 1782211143311294464
author Landry, Glenn J.
Kent, Brianne A.
Patton, Danica F.
Jaholkowski, Mark
Marchant, Elliott G.
Mistlberger, Ralph E.
author_facet Landry, Glenn J.
Kent, Brianne A.
Patton, Danica F.
Jaholkowski, Mark
Marchant, Elliott G.
Mistlberger, Ralph E.
author_sort Landry, Glenn J.
collection PubMed
description The dorsomedial hypothalamus (DMH) is a site of circadian clock gene and immediate early gene expression inducible by daytime restricted feeding schedules that entrain food anticipatory circadian rhythms in rats and mice. The role of the DMH in the expression of anticipatory rhythms has been evaluated using different lesion methods. Partial lesions created with the neurotoxin ibotenic acid (IBO) have been reported to attenuate food anticipatory rhythms, while complete lesions made with radiofrequency current leave anticipatory rhythms largely intact. We tested a hypothesis that the DMH and fibers of passage spared by IBO lesions play a time-of-day dependent role in the expression of food anticipatory rhythms. Rats received intra-DMH microinjections of IBO and activity and body temperature (T (b)) rhythms were recorded by telemetry during ad-lib food access, total food deprivation and scheduled feeding, with food provided for 4-h/day for 20 days in the middle of the light period and then for 20 days late in the dark period. During ad-lib food access, rats with DMH lesions exhibited a lower amplitude and mean level of light-dark entrained activity and T (b) rhythms. During the daytime feeding schedule, all rats exhibited food anticipatory activity and T (b) rhythms that persisted during 2 days without food in constant dark. In some rats with partial or total DMH ablation, the magnitude of the anticipatory rhythm was weak relative to most intact rats. When mealtime was shifted to the late night, the magnitude of the food anticipatory activity rhythms in these cases was restored to levels characteristic of intact rats. These results confirm that rats can anticipate scheduled daytime or nighttime meals without the DMH. Improved anticipation at night suggests a modulatory role for the DMH in the expression of food anticipatory activity rhythms during the daily light period, when nocturnal rodents normally sleep.
format Online
Article
Text
id pubmed-3166290
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-31662902011-09-12 Evidence for Time-of-Day Dependent Effect of Neurotoxic Dorsomedial Hypothalamic Lesions on Food Anticipatory Circadian Rhythms in Rats Landry, Glenn J. Kent, Brianne A. Patton, Danica F. Jaholkowski, Mark Marchant, Elliott G. Mistlberger, Ralph E. PLoS One Research Article The dorsomedial hypothalamus (DMH) is a site of circadian clock gene and immediate early gene expression inducible by daytime restricted feeding schedules that entrain food anticipatory circadian rhythms in rats and mice. The role of the DMH in the expression of anticipatory rhythms has been evaluated using different lesion methods. Partial lesions created with the neurotoxin ibotenic acid (IBO) have been reported to attenuate food anticipatory rhythms, while complete lesions made with radiofrequency current leave anticipatory rhythms largely intact. We tested a hypothesis that the DMH and fibers of passage spared by IBO lesions play a time-of-day dependent role in the expression of food anticipatory rhythms. Rats received intra-DMH microinjections of IBO and activity and body temperature (T (b)) rhythms were recorded by telemetry during ad-lib food access, total food deprivation and scheduled feeding, with food provided for 4-h/day for 20 days in the middle of the light period and then for 20 days late in the dark period. During ad-lib food access, rats with DMH lesions exhibited a lower amplitude and mean level of light-dark entrained activity and T (b) rhythms. During the daytime feeding schedule, all rats exhibited food anticipatory activity and T (b) rhythms that persisted during 2 days without food in constant dark. In some rats with partial or total DMH ablation, the magnitude of the anticipatory rhythm was weak relative to most intact rats. When mealtime was shifted to the late night, the magnitude of the food anticipatory activity rhythms in these cases was restored to levels characteristic of intact rats. These results confirm that rats can anticipate scheduled daytime or nighttime meals without the DMH. Improved anticipation at night suggests a modulatory role for the DMH in the expression of food anticipatory activity rhythms during the daily light period, when nocturnal rodents normally sleep. Public Library of Science 2011-09-02 /pmc/articles/PMC3166290/ /pubmed/21912674 http://dx.doi.org/10.1371/journal.pone.0024187 Text en Landry et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Landry, Glenn J.
Kent, Brianne A.
Patton, Danica F.
Jaholkowski, Mark
Marchant, Elliott G.
Mistlberger, Ralph E.
Evidence for Time-of-Day Dependent Effect of Neurotoxic Dorsomedial Hypothalamic Lesions on Food Anticipatory Circadian Rhythms in Rats
title Evidence for Time-of-Day Dependent Effect of Neurotoxic Dorsomedial Hypothalamic Lesions on Food Anticipatory Circadian Rhythms in Rats
title_full Evidence for Time-of-Day Dependent Effect of Neurotoxic Dorsomedial Hypothalamic Lesions on Food Anticipatory Circadian Rhythms in Rats
title_fullStr Evidence for Time-of-Day Dependent Effect of Neurotoxic Dorsomedial Hypothalamic Lesions on Food Anticipatory Circadian Rhythms in Rats
title_full_unstemmed Evidence for Time-of-Day Dependent Effect of Neurotoxic Dorsomedial Hypothalamic Lesions on Food Anticipatory Circadian Rhythms in Rats
title_short Evidence for Time-of-Day Dependent Effect of Neurotoxic Dorsomedial Hypothalamic Lesions on Food Anticipatory Circadian Rhythms in Rats
title_sort evidence for time-of-day dependent effect of neurotoxic dorsomedial hypothalamic lesions on food anticipatory circadian rhythms in rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3166290/
https://www.ncbi.nlm.nih.gov/pubmed/21912674
http://dx.doi.org/10.1371/journal.pone.0024187
work_keys_str_mv AT landryglennj evidencefortimeofdaydependenteffectofneurotoxicdorsomedialhypothalamiclesionsonfoodanticipatorycircadianrhythmsinrats
AT kentbriannea evidencefortimeofdaydependenteffectofneurotoxicdorsomedialhypothalamiclesionsonfoodanticipatorycircadianrhythmsinrats
AT pattondanicaf evidencefortimeofdaydependenteffectofneurotoxicdorsomedialhypothalamiclesionsonfoodanticipatorycircadianrhythmsinrats
AT jaholkowskimark evidencefortimeofdaydependenteffectofneurotoxicdorsomedialhypothalamiclesionsonfoodanticipatorycircadianrhythmsinrats
AT marchantelliottg evidencefortimeofdaydependenteffectofneurotoxicdorsomedialhypothalamiclesionsonfoodanticipatorycircadianrhythmsinrats
AT mistlbergerralphe evidencefortimeofdaydependenteffectofneurotoxicdorsomedialhypothalamiclesionsonfoodanticipatorycircadianrhythmsinrats