Cargando…
Chopper Is Prodeath Regardless of the Effect of p75ICD on Sensitivity to Oxidative Stress
Background. The intracellular domain (ICD) of the neurotrophin receptor, p75NTR, exhibits variably pro- and antiapoptotic activity and has been implicated in neurodegenerative and neurodestructive disease. The molecular determinants of these cellular effects are not completely understood. The “Chopp...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3166776/ https://www.ncbi.nlm.nih.gov/pubmed/21904642 http://dx.doi.org/10.1155/2011/391659 |
_version_ | 1782211185345560576 |
---|---|
author | Qazi, Alliya Halterman, Marc W. Mi, Zhiping Zhang, Tong Schor, Nina F. |
author_facet | Qazi, Alliya Halterman, Marc W. Mi, Zhiping Zhang, Tong Schor, Nina F. |
author_sort | Qazi, Alliya |
collection | PubMed |
description | Background. The intracellular domain (ICD) of the neurotrophin receptor, p75NTR, exhibits variably pro- and antiapoptotic activity and has been implicated in neurodegenerative and neurodestructive disease. The molecular determinants of these cellular effects are not completely understood. The “Chopper” domain of p75ICD has been shown to be proapoptotic in in vitro systems in which p75ICD is proapoptotic. The effects of Chopper in systems in which p75ICD is antiapoptotic and, therefore, whether or not Chopper accounts for the variability of the cellular effects of p75ICD are not known. We therefore examined the effects of deletion of Chopper on the effects of p75ICD on in vitro cell culture systems in which p75ICD is pro- or antiapoptotic, respectively. Results. In HN33.11 murine neuroblastoma-hippocampal neuron hybrid cells, p75ICD is antiapoptotic. In NIH 3T3 cells, p75ICD is proapoptotic. In both cell lines deletion of the Chopper domain from p75ICD decreases the incidence of apoptosis resulting from oxidative stress. Thus, irrespective of the nature of the effects of p75ICD on the cell, its Chopper domain is proapoptotic. Conclusions. Expression of p75ICD can enhance or attenuate oxidative induction of apoptosis. Variability of the effects of p75ICD is not related to variability of the effects of its Chopper domain. |
format | Online Article Text |
id | pubmed-3166776 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-31667762011-09-08 Chopper Is Prodeath Regardless of the Effect of p75ICD on Sensitivity to Oxidative Stress Qazi, Alliya Halterman, Marc W. Mi, Zhiping Zhang, Tong Schor, Nina F. Oxid Med Cell Longev Research Article Background. The intracellular domain (ICD) of the neurotrophin receptor, p75NTR, exhibits variably pro- and antiapoptotic activity and has been implicated in neurodegenerative and neurodestructive disease. The molecular determinants of these cellular effects are not completely understood. The “Chopper” domain of p75ICD has been shown to be proapoptotic in in vitro systems in which p75ICD is proapoptotic. The effects of Chopper in systems in which p75ICD is antiapoptotic and, therefore, whether or not Chopper accounts for the variability of the cellular effects of p75ICD are not known. We therefore examined the effects of deletion of Chopper on the effects of p75ICD on in vitro cell culture systems in which p75ICD is pro- or antiapoptotic, respectively. Results. In HN33.11 murine neuroblastoma-hippocampal neuron hybrid cells, p75ICD is antiapoptotic. In NIH 3T3 cells, p75ICD is proapoptotic. In both cell lines deletion of the Chopper domain from p75ICD decreases the incidence of apoptosis resulting from oxidative stress. Thus, irrespective of the nature of the effects of p75ICD on the cell, its Chopper domain is proapoptotic. Conclusions. Expression of p75ICD can enhance or attenuate oxidative induction of apoptosis. Variability of the effects of p75ICD is not related to variability of the effects of its Chopper domain. Hindawi Publishing Corporation 2011 2011-09-05 /pmc/articles/PMC3166776/ /pubmed/21904642 http://dx.doi.org/10.1155/2011/391659 Text en Copyright © 2011 Alliya Qazi et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Qazi, Alliya Halterman, Marc W. Mi, Zhiping Zhang, Tong Schor, Nina F. Chopper Is Prodeath Regardless of the Effect of p75ICD on Sensitivity to Oxidative Stress |
title | Chopper Is Prodeath Regardless of the Effect of p75ICD on Sensitivity to Oxidative Stress |
title_full | Chopper Is Prodeath Regardless of the Effect of p75ICD on Sensitivity to Oxidative Stress |
title_fullStr | Chopper Is Prodeath Regardless of the Effect of p75ICD on Sensitivity to Oxidative Stress |
title_full_unstemmed | Chopper Is Prodeath Regardless of the Effect of p75ICD on Sensitivity to Oxidative Stress |
title_short | Chopper Is Prodeath Regardless of the Effect of p75ICD on Sensitivity to Oxidative Stress |
title_sort | chopper is prodeath regardless of the effect of p75icd on sensitivity to oxidative stress |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3166776/ https://www.ncbi.nlm.nih.gov/pubmed/21904642 http://dx.doi.org/10.1155/2011/391659 |
work_keys_str_mv | AT qazialliya chopperisprodeathregardlessoftheeffectofp75icdonsensitivitytooxidativestress AT haltermanmarcw chopperisprodeathregardlessoftheeffectofp75icdonsensitivitytooxidativestress AT mizhiping chopperisprodeathregardlessoftheeffectofp75icdonsensitivitytooxidativestress AT zhangtong chopperisprodeathregardlessoftheeffectofp75icdonsensitivitytooxidativestress AT schorninaf chopperisprodeathregardlessoftheeffectofp75icdonsensitivitytooxidativestress |