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Plasma proteomic profile of sulfur mustard exposed lung diseases patients using 2-dimensional gel electrophoresis

INTRODUCTION: Sulfur mustard "bis (2-chlroethyl) sulphide" (SM) is a chemical warfare agent that remains a threat to human health. The aim of this study was to identify protein expression signature or biomarkers that reflect chronic lung damages induced by SM exposure. METHODS: Prior to an...

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Detalles Bibliográficos
Autores principales: Mehrani, Hossein, Ghanei, Mostafa, Aslani, Jafar, Tabatabaei, Zahra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3167199/
https://www.ncbi.nlm.nih.gov/pubmed/21906349
http://dx.doi.org/10.1186/1559-0275-8-2
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author Mehrani, Hossein
Ghanei, Mostafa
Aslani, Jafar
Tabatabaei, Zahra
author_facet Mehrani, Hossein
Ghanei, Mostafa
Aslani, Jafar
Tabatabaei, Zahra
author_sort Mehrani, Hossein
collection PubMed
description INTRODUCTION: Sulfur mustard "bis (2-chlroethyl) sulphide" (SM) is a chemical warfare agent that remains a threat to human health. The aim of this study was to identify protein expression signature or biomarkers that reflect chronic lung damages induced by SM exposure. METHODS: Prior to analysis, plasma was fractionated using ethanol precipitation. Using two dimensional SDS-PAGE; fractionated protein profiles of 20 healthy and 20 exposed patients with lung diseases were established. Selected protein spots were successfully identified with MALDI TOF MS/MS. RESULTS: The results show that α1 haptoglobin isoforms were detected in plasma of the all lung disease patients but none of the healthy controls. Amyloid A1 isoforms was also detected in plasma of the lung disease patients but none of the healthy controls. Moreover, low molecular weight proteins were enriched in ethanol supernatant compared to ethanol precipitate. CONCLUSION: Our present results and previous studies suggest that ongoing tissue remodeling is involved in SM exposed lung damage patients. These finding might improve patient care and suitable therapies.
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spelling pubmed-31671992011-09-06 Plasma proteomic profile of sulfur mustard exposed lung diseases patients using 2-dimensional gel electrophoresis Mehrani, Hossein Ghanei, Mostafa Aslani, Jafar Tabatabaei, Zahra Clin Proteomics Research INTRODUCTION: Sulfur mustard "bis (2-chlroethyl) sulphide" (SM) is a chemical warfare agent that remains a threat to human health. The aim of this study was to identify protein expression signature or biomarkers that reflect chronic lung damages induced by SM exposure. METHODS: Prior to analysis, plasma was fractionated using ethanol precipitation. Using two dimensional SDS-PAGE; fractionated protein profiles of 20 healthy and 20 exposed patients with lung diseases were established. Selected protein spots were successfully identified with MALDI TOF MS/MS. RESULTS: The results show that α1 haptoglobin isoforms were detected in plasma of the all lung disease patients but none of the healthy controls. Amyloid A1 isoforms was also detected in plasma of the lung disease patients but none of the healthy controls. Moreover, low molecular weight proteins were enriched in ethanol supernatant compared to ethanol precipitate. CONCLUSION: Our present results and previous studies suggest that ongoing tissue remodeling is involved in SM exposed lung damage patients. These finding might improve patient care and suitable therapies. Springer 2011-01-07 /pmc/articles/PMC3167199/ /pubmed/21906349 http://dx.doi.org/10.1186/1559-0275-8-2 Text en Copyright ©2011 Mehrani et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Mehrani, Hossein
Ghanei, Mostafa
Aslani, Jafar
Tabatabaei, Zahra
Plasma proteomic profile of sulfur mustard exposed lung diseases patients using 2-dimensional gel electrophoresis
title Plasma proteomic profile of sulfur mustard exposed lung diseases patients using 2-dimensional gel electrophoresis
title_full Plasma proteomic profile of sulfur mustard exposed lung diseases patients using 2-dimensional gel electrophoresis
title_fullStr Plasma proteomic profile of sulfur mustard exposed lung diseases patients using 2-dimensional gel electrophoresis
title_full_unstemmed Plasma proteomic profile of sulfur mustard exposed lung diseases patients using 2-dimensional gel electrophoresis
title_short Plasma proteomic profile of sulfur mustard exposed lung diseases patients using 2-dimensional gel electrophoresis
title_sort plasma proteomic profile of sulfur mustard exposed lung diseases patients using 2-dimensional gel electrophoresis
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3167199/
https://www.ncbi.nlm.nih.gov/pubmed/21906349
http://dx.doi.org/10.1186/1559-0275-8-2
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