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Plasma proteomic profile of sulfur mustard exposed lung diseases patients using 2-dimensional gel electrophoresis
INTRODUCTION: Sulfur mustard "bis (2-chlroethyl) sulphide" (SM) is a chemical warfare agent that remains a threat to human health. The aim of this study was to identify protein expression signature or biomarkers that reflect chronic lung damages induced by SM exposure. METHODS: Prior to an...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3167199/ https://www.ncbi.nlm.nih.gov/pubmed/21906349 http://dx.doi.org/10.1186/1559-0275-8-2 |
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author | Mehrani, Hossein Ghanei, Mostafa Aslani, Jafar Tabatabaei, Zahra |
author_facet | Mehrani, Hossein Ghanei, Mostafa Aslani, Jafar Tabatabaei, Zahra |
author_sort | Mehrani, Hossein |
collection | PubMed |
description | INTRODUCTION: Sulfur mustard "bis (2-chlroethyl) sulphide" (SM) is a chemical warfare agent that remains a threat to human health. The aim of this study was to identify protein expression signature or biomarkers that reflect chronic lung damages induced by SM exposure. METHODS: Prior to analysis, plasma was fractionated using ethanol precipitation. Using two dimensional SDS-PAGE; fractionated protein profiles of 20 healthy and 20 exposed patients with lung diseases were established. Selected protein spots were successfully identified with MALDI TOF MS/MS. RESULTS: The results show that α1 haptoglobin isoforms were detected in plasma of the all lung disease patients but none of the healthy controls. Amyloid A1 isoforms was also detected in plasma of the lung disease patients but none of the healthy controls. Moreover, low molecular weight proteins were enriched in ethanol supernatant compared to ethanol precipitate. CONCLUSION: Our present results and previous studies suggest that ongoing tissue remodeling is involved in SM exposed lung damage patients. These finding might improve patient care and suitable therapies. |
format | Online Article Text |
id | pubmed-3167199 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Springer |
record_format | MEDLINE/PubMed |
spelling | pubmed-31671992011-09-06 Plasma proteomic profile of sulfur mustard exposed lung diseases patients using 2-dimensional gel electrophoresis Mehrani, Hossein Ghanei, Mostafa Aslani, Jafar Tabatabaei, Zahra Clin Proteomics Research INTRODUCTION: Sulfur mustard "bis (2-chlroethyl) sulphide" (SM) is a chemical warfare agent that remains a threat to human health. The aim of this study was to identify protein expression signature or biomarkers that reflect chronic lung damages induced by SM exposure. METHODS: Prior to analysis, plasma was fractionated using ethanol precipitation. Using two dimensional SDS-PAGE; fractionated protein profiles of 20 healthy and 20 exposed patients with lung diseases were established. Selected protein spots were successfully identified with MALDI TOF MS/MS. RESULTS: The results show that α1 haptoglobin isoforms were detected in plasma of the all lung disease patients but none of the healthy controls. Amyloid A1 isoforms was also detected in plasma of the lung disease patients but none of the healthy controls. Moreover, low molecular weight proteins were enriched in ethanol supernatant compared to ethanol precipitate. CONCLUSION: Our present results and previous studies suggest that ongoing tissue remodeling is involved in SM exposed lung damage patients. These finding might improve patient care and suitable therapies. Springer 2011-01-07 /pmc/articles/PMC3167199/ /pubmed/21906349 http://dx.doi.org/10.1186/1559-0275-8-2 Text en Copyright ©2011 Mehrani et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Mehrani, Hossein Ghanei, Mostafa Aslani, Jafar Tabatabaei, Zahra Plasma proteomic profile of sulfur mustard exposed lung diseases patients using 2-dimensional gel electrophoresis |
title | Plasma proteomic profile of sulfur mustard exposed lung diseases patients using 2-dimensional gel electrophoresis |
title_full | Plasma proteomic profile of sulfur mustard exposed lung diseases patients using 2-dimensional gel electrophoresis |
title_fullStr | Plasma proteomic profile of sulfur mustard exposed lung diseases patients using 2-dimensional gel electrophoresis |
title_full_unstemmed | Plasma proteomic profile of sulfur mustard exposed lung diseases patients using 2-dimensional gel electrophoresis |
title_short | Plasma proteomic profile of sulfur mustard exposed lung diseases patients using 2-dimensional gel electrophoresis |
title_sort | plasma proteomic profile of sulfur mustard exposed lung diseases patients using 2-dimensional gel electrophoresis |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3167199/ https://www.ncbi.nlm.nih.gov/pubmed/21906349 http://dx.doi.org/10.1186/1559-0275-8-2 |
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